Subscribe Now Subscribe Today
Research Article
 

Fractionation and Characterization of Seed Storage Proteins from Different Wheat Varieties Cultivated in Sindh on SDS-PAGE Electrophoresis



Shaista Khan, A.N. Memon, Allah Bux Ghanghro and Ibtessam Tahir
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

Wheat is most widely cultivated cereal and important source of food and protein worldwide. Wheat is a staple diet containing most of the ingredient, for wide range of food. The unique property of wheat flour is to form dough and this property is due to gluten required for making preparation of chapatti and bread. Fractionation and characterization of seed storage proteins of fourteen wheat varieties cultivated in sindh was carried out by Sodium Dodecyle Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Wheat grains of fourteen (14) varieties were collected from different region of sindh (Pakistan). Electrogram of each variety were calculated by Jaccard’s Similarity Index (JSI). Genetic diversity of different sindh wheat varieties was measured by constructing dendogram of High Molecular Weight (HMW) and Low Molecular Weight (LMW) gluten sub unit bands. The dendrogram profile data showed the variation in the number and position of bands from one variety to the other, while some bands are common.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Shaista Khan, A.N. Memon, Allah Bux Ghanghro and Ibtessam Tahir, 2011. Fractionation and Characterization of Seed Storage Proteins from Different Wheat Varieties Cultivated in Sindh on SDS-PAGE Electrophoresis. Pakistan Journal of Nutrition, 10: 139-142.

DOI: 10.3923/pjn.2011.139.142

URL: https://scialert.net/abstract/?doi=pjn.2011.139.142

REFERENCES
1:  Bietz, J.A. and D.G. Simpson, 1992. Electrophoresis and chromatography of wheat proteins: Available methods and procedures for statistical evaluation of the data. J. Chromatogr., 624: 53-80.
CrossRef  |  PubMed  |  Direct Link  |  

2:  Branlard, G., J.C. Aulran and P. Monneveux, 1989. High molecular weight glutenin subunit in durum wheat (T. durum). Theor. Applied Genet., 78: 353-358.
CrossRef  |  

3:  Cooke, R.J. and J.R. Law, 1998. Seed storage protein diversity in wheat varieties. Plant Varieties Seeds, 11: 159-167.

4:  Haridas Rao, P., K. Leelavathi and S.R. Shurpalekar, 1986. Objective measurements of the consistency of chapati dough using research water absorptions meter. J. Textural Stud., 17: 401-420.
CrossRef  |  Direct Link  |  

5:  Khan, M.F., E. Schumann and W.E. Weber, 2002. Characterisation of Pakistani wheat varieties for general cultivation in the mountaineous regions of Azad Kashmir. Asian J. Plant Sci., 1: 699-702.
CrossRef  |  Direct Link  |  

6:  Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
CrossRef  |  Direct Link  |  

7:  Lawrence, G.J. and K.W. Shepherd, 1980. Variation in glutenin protein subunits of wheat. Aust. J. Biol. Sci., 33: 221-233.
Direct Link  |  

8:  MacRitchie, F., 1994. Role of Polymeric Proteins in Flour Functionality in Wheat Kernel Proteins: Molecular and Functional Aspects. Universita Degli Studi Della Tuscia, Bitervo, Italy, pp: 145-150.

9:  Payne, P.I., L.M. Holt and C.N. Law, 1981. Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part I: Alleic variation in subunits amongst varieties of wheat (T. aestivum). Theor. Applied Genet., 60: 229-236.
CrossRef  |  Direct Link  |  

10:  Payne, P.I., C.N. Law and E.E. Mudd, 1980. Control by homoeologous group 1 chromosome of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor. Applied Genet., 58: 113-120.

11:  Woychik, H.J., J.A. Boundy and R.J. Dimler, 1961. Starch gel electrophoresis of wheat gluten proteins with concentrated urea. Arch. Biochem. Biophys., 94: 477-482.
CrossRef  |  

12:  Shuaib, M., A. Zeb, Z. Ali, W. Ali, T. Ahmad and I. Khan, 2007. Characterization of wheat varieties by seed storage protein electrophoresis. Afr. J. Biotechnol., 6: 497-500.
Direct Link  |  

©  2021 Science Alert. All Rights Reserved