ABSTRACT
The aim of this study was to investigate effects of different levels of Clove Methanol Extract (CME) on methane production in ruminant with practical diets. Two experimental diets (diet1: 100% forage and diet 2: 100% barley) were used to investigate effects of 3 levels of CME (0, 0.5 and 1 ml) on methane production at 2, 4, 12, 16, 24, 48, 72 and 96 h of incubation. Gas production was continuously measured by EX-TEC HS 680 GC. Cumulative gas production was recorded at 2, 4, 12, 16, 24, 48, 72 and 96 h of incubation periods. Data processed in excel and analyzed using SAS statistical analyze software. Result show that Clove methanol extract in initial time of incubation (2 and 4 h) not affects on the decreased of methane production, but increase incubation time CME significantly decreased methane gas volume in two diets and all of CME levels and could significantly decrease methane volume in the treatment. According to result we could suggest CME for improve methane production in the in vitro condition and need further research for application CME in field of ruminant nutrition.
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Ali Halimi Shabestari, Ramin Salamatdoustnobar, Naser Maheri-Sis, Abolfazl Gorbani, Kamal Mirzadeh Ahari, Ali Noshadi, Hossien Samadi and Jafar Salimi Nezhad, 2011. Evaluation Effects of Clove Methanol Extract on Methane Production in the in vitro Condition. Pakistan Journal of Nutrition, 10: 1154-1157.
DOI: 10.3923/pjn.2011.1154.1157
URL: https://scialert.net/abstract/?doi=pjn.2011.1154.1157
DOI: 10.3923/pjn.2011.1154.1157
URL: https://scialert.net/abstract/?doi=pjn.2011.1154.1157
REFERENCES
- Baker, S.K., 1999. Rumen methanogens and inhibition of methanogenesis. Aust. J. Agric. Res., 50: 1293-1298.
CrossRefDirect Link - Busquet, M., S. Calsamiglia, A. Ferret and C. Kamel, 2006. Plant extracts affect in vitro rumen microbial fermentation. J. Dairy Sci., 89: 761-771.
CrossRefDirect Link - Calsamiglia, S., M. Busquet, P.W. Cardozo, L. Castillejos and A. Ferret, 2007. Essential oils as modifiers of rumen microbial fermentation. J. Dairy Sci., 90: 2580-2595.
CrossRefPubMedDirect Link - Guo, Y.Q., W.L. Hu and J.X. Liu, 2005. Methanogens and manipulation of methane production in the rumen. Wei Sheng Wu Xue Bao., 45: 145-148.
PubMedDirect Link - Huang, Y., S.H. Ho, H.C. Lee and Y.L. Yap, 2002. Insecticidal properties of eugenol, isoeugenol and methyleugenol and their effects on nutrition of Sitophilus zeamais Motsch. (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). J. Stored Prod. Res., 38: 403-412.
CrossRefDirect Link - Johnson, K.A. and D.E. Johnson, 1995. Methane emissions from cattle. J. Anim. Sci., 73: 2483-2492.
PubMedDirect Link - Kumar, S., A. Puniya, M. Puniya, S. Dagar, S. Sirohi, K. Singh and G. Griffith, 2009. Factors affecting rumen methanogens and methane mitigation strategies. World J. Microbiol. Biotechnol., 25: 1557-1566.
Direct Link - Mastepanov, M., C. Sigsgaard and E. Dlugokencky, 2008. Large tundra methane burst during onset of freezing. Nat. Prod. Lett., 456: 628-631.
Direct Link - Menke, K.H. and H. Steingass, 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev., 28: 7-55.
Direct Link - Moss, A.R., J.P. Jouany and J. Newbold, 2000. Methane production by ruminants: Its contribution to global warming. Annales Zootechnie, 49: 231-253.
CrossRefDirect Link - Patra, A.K., D.N. Kamra and N. Agarwal, 2006. Effect of plant extracts on In vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo. Anim. Feed Sci. Technol., 128: 276-291.
CrossRefDirect Link - Patra, A.K., D.N. Kamra and N. Agarwal, 2006. Effect of spices on rumen fermentation, methanogenesis and protozoa counts in in vitro gas production test. Int. Cong. Ser., 1293: 176-179.
Direct Link - Patra, A.K. and J. Saxena, 2011. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. J. Sci. Food Agric., 91: 24-37.
CrossRefPubMedDirect Link - Sirohi, S.K., N. Pandey, N. Goel, B. Singh, M. Mohini, P. Pandey and P.P. Chaudhry, 2009. Microbial activity and ruminal methanogenesis as affected by plant secondary metabolites in different plant extracts. Int. J. Environ. Sci. Eng., 1: 52-58.
Direct Link - Teferedegne, B., 2000. New perspectives on the use of tropical plants to improve ruminant nutrition. Proc. Nutr. Soc., 59: 209-214.
CrossRefPubMedDirect Link - Ungerfeld, E.M., S.R. Rust and D.R. Boone, 2004. Effects of several inhibitors on pure cultures of ruminal methanogens. J. Appl. Microbiol., 97: 520-526.
PubMedDirect Link - Woese, C.R., O. Kandler and M.L. Wheelis, 1990. Towards a natural system of organisms: Proposal for the domains Archaea, Bacteria and Eucarya. Proc. Natl. Acad. Sci. USA., 87: 4576-4579.
Direct Link - Wright, A.D., G.A.J. Williams, B. Winder, C. Christophersen, S. Rodgers and K. Smith, 2004. Molecular diversity of rumen methanogens from sheep in Western Australia. Applied Environ. Microbiol., 70: 1263-1270.
CrossRef