Subscribe Now Subscribe Today
Fulltext PDF
Research Article

Effect of Some Dietary Oils and Fats on Serum Lipid Profile, Calcium Absorption and Bone Mineralization in Mice

Amr A. Rezq, Fatma A. Labib and Abd Elrahman M. Attia

Amount and type of fats in the diet have an important effect on bone health and lipid profile. This study was conducted to investigate the effect of different types of dietary oils and fats on lipid profile, calcium absorption and bone mineralization in male mice. Mice weighing 25±5 g were divided into nine groups and fed on diets without oils or fats (control group) and containing soybean oil, corn oil, olive oil, palm oil, sunflower oil, butter, animal fat or margarine. Mice fed on diet containing soybean oil or olive oil had the lowest levels of TG, TC, LDL-c and HDL-c as compared to the other groups. Diets with palm oil, olive oil, sunflower oil, butter, animal fat or margarine caused significant decreases in the serum level of calcium as compared to the effect of diet without oils or fats. Mice fed diet containing olive oil, butter or animal fat had significant increase in bone density, while those fed diet containing soybean oil, corn oil, sunflower oil or margarine had significant decreases in femur bone density, compared to the control group. The apparent calcium absorption was significantly increased by feeding diets containing soybean oil, corn oil, palm oil, olive oil, sunflower oil, butter or animal fat. Dietary intake of vegetable oils improved lipid profile while butter, animal fat and margarine had the opposite effect. Butter and animal fats increased calcium and phosphorus deposition in femur bone more than vegetable oils.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Amr A. Rezq, Fatma A. Labib and Abd Elrahman M. Attia, 2010. Effect of Some Dietary Oils and Fats on Serum Lipid Profile, Calcium Absorption and Bone Mineralization in Mice. Pakistan Journal of Nutrition, 9: 643-650.

DOI: 10.3923/pjn.2010.643.650


AAPCN, 1998. Pediatric Nutrition. American Academy of Pediatrics, Committee on Nutrition, Village, IL, pp: 653-654.

Annemieke, M.B., A.M. Melanie, G.J. Engels, E.P. Boerma and M.P. Sabine, 1997. Changes in bone mineral density, body composition and lipid metabolism during Growth Hormone (GH) treatment in children with GH deficiency. J. Clin. Endocrinol. Metabol., 82: 2423-2428.
Direct Link  |  

Asadi, F., A. Shahriari, M. Pourkabir and R. Maclaren, 2008. Short and long-term effects of corn oil on serum lipid and lipoprotein and visceral abdominal in rats. J. Food Lipi., 15: 68-80.
CrossRef  |  Direct Link  |  

Beynen, A.C., J.J. Visser, J.A. Schouten and M.B. Katan, 1987. Cholesterol metabolism in rabbits fed diets containing either corn oil or olive oil. Nutr. Rep. Intern., 35: 111-116.

Brownbill, R.A., M. Petrosian and J.Z. Ilich, 2005. Association between dietary conjugated linoleic acid and bone mineral density in postmenopausal women. J. Am. Coll. Nutr., 24: 177-181.
Direct Link  |  

Chin, S.F., W. Liu and J.M. Storkson, 1992. Advances in conjugated linoleic acid research. J. Food Comp. Anal., 5: 185-185.

Choi, Y., C. Ahn, H. Rhee, M. Choe, C. Kim and J. Kim, 1993. Comparative effects of dietary palm oil, perilla oil and soybean oil on lipid profile in different aged rats fed on hypercholesterolemic diets. Bioscui. Biotech. Biochem., 57: 65-68.

Doyle, L. and K.D. Cashman, 2003. The effect of nutrient profiles of the dietary approaches to stop hypertension diets on blood pressure and bone metabolism and composition in normotensive and hypertensive rats. Br. J. Nutr., 89: 713-724.

Haag, M., O.N. Magada, N. Claassen, L.H. Bohmer and M.C. Kruger, 2003. Omega-3 fatty acids modulate ATPases involved in duodenal calcium absorption. Prostaglandins Leukot Essent Fatty Acids., 68: 423-429.
PubMed  |  

Hayes, K.C., A. Pronezuk, S. Lindsey and D. Diersen-Schade, 1991. Dietary saturated fatty acids (12:0, 14:0, 16:0) differ in their impact on plasma cholesterol and lipoproteins in nonhuman primates. Am. J. Clin. Nutr., 53: 491-498.
Direct Link  |  

Hoffman, R., L. Lawrence, D. Kronfeld, W. Cooper, D. Sklan and J. Dascanio, 1999. Dietary carbohydrates, and fat influence radiographic bone mineral content in growing foals. J. Anim. Sci., 77: 3330-3338.
Direct Link  |  

Ima-Nirwana, S., A. Kiftiah, T. Sariza, M.T. Gapor and B.A.K. Khalid, 1999. Palm vitamin E improves bone metabolism and survival rate in thyrotoxic rats. Gen. Pharm., 32: 621-626.
CrossRef  |  

Jie, M.Z., S. Shimanuki, A. Igarashi, Y. Kawasaki and M. Yamaguchi, 2000. Preventive effect of dietary soybean on bone Loss in ovariectomized rats: Enhancement with isoflavone and zinc supplementation. J. Health Sci., 46: 263-268.
Direct Link  |  

Kanis, J.A., 1999. The use of Ca in the management of osteoporosis. Bone, 24: 279-290.
PubMed  |  

Karaji-Bani, M., F. Montazeri and M. Hashemi, 2006. Effect of palm oil on serum lipid profile in rats. Pak. J. Nutr., 5: 234-236.
CrossRef  |  Direct Link  |  

Kris-Etheton, P.M. and S. Yu, 1997. Individual fatty acid, effect on plasma lipids and lipoproteins: Human studies. Am. J. Clin. Nutr., 65: 1628S-1644S.
Direct Link  |  

Lee, K.N., D. Kritchevsky and M.W. Pariza, 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis, 108: 19-25.
CrossRef  |  PubMed  |  Direct Link  |  

Lichtenstein, A.H., L.M. Ausman, W. Carrasco, J.L. Jenner, J.M. Ordovas and E.J. Schaefer, 1993. Hydrogenation impairs the hypolipidemic effect of corn oil in humans. Hydrogenation, trans fatty acids and plasma lipids. Arterioscler Thromb, 13: 154-161.
PubMed  |  

Liu, D., H.P. Veit, J.H. Wilson and D.M. Denbow, 2003. Long-term supplementation of various dietary lipids alter bone mineral content, mechanical properties and histological characteristics of Japanese quail. Poult. Sci., 82: 831-839.
PubMed  |  

Mensink, R.P. and M.B. Katan, 1990. Effect of dietary tram fatty acids on high-density and low-density lipoprotein cholesterol levels in healthy subjects. N. Engl. J. Med., 323: 439-445.
PubMed  |  

Mjyazawa, E.T. and Yoshida, 1991. Effect of dietary levels of phytate and inorganic phosphate on phytate breakdown and absorption of calcium and magnesium in rats. Nutr. Res., 11: 797-806.

Mollard, R.C. and H.A. Weiler, 2006. Dietary arachidonic acid and docosahexaenoic acid elevate femur calcium and reduce zinc content in piglets. Pediatr. Res., 60: 418-422.
PubMed  |  

Morello, J.R., M.J. Motilva, M.J. Tovar and M.P. Romero, 2007. Changes in commercial virgin olive oil during storage, with special emphasis on the phenolic fraction. J. Agric. Food Chem., 85: 357-364.

Norazlina, M., S. Ima-Nirwana, M.T. Abd Gapor and B.A.K. Khalid, 2002. Tocotrienols are needed for normal bone calcification in growing female rats. Asia Pacific J. Clin. Nutr., 11: 194-199.
CrossRef  |  PubMed  |  

Oluba, O.M., O. Adeyemi, G.C. Ojieh, C.O. Aboluwoye and G.O. Eidangbe, 2008. Comparative effect of soybean oil and palm oil on serum lipids and some serum enzymes in cholesterol-fed rats. Eur. J. Sci. Res., 23: 559-566.
Direct Link  |  

Parhami, F., 2003. Possible role of oxidized lipids in osteoporosis: Could hyperlipidemia be a risk factor: Prostaglandins Leukot Essent. Fatty Acids, 68: 373-378.
PubMed  |  

Perez-Granados, A.M., M.P. Vaquero and M.P. Navrro, 2006. Calcium absorption in rats consuming olive oil or sunflower oil unused or used in frying. J. Food Sci., 65: 892-896.

Puela, C., A. Quintina, A. Agaliasa, J. Matheya, C. Obleda and A. Mazura, 2004. Olive oil and its main phenolic micronutrient (oleuropein) prevent inflammation-induced bone loss in the ovariectomised rat. Br. J. Nutr., 92: 119-127.
PubMed  |  

Ramadan, M.A., M.A. Amer and A.E. Awad, 2008. Coriander seed and soybean oils improve plasma lipid profile in rats fed a diet containing cholesterol. Eur. Food Res. Technol., 227: 1173-1182.

Reeves, P.G., F.H. Nielsen and G.C. Fahey Jr., 1993. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr., 123: 1939-1951.
CrossRef  |  PubMed  |  Direct Link  |  

Scholtz, S.C., M. Pieters, W. Oosthuizen, J.C. Jerling, M.J. Bosman and H.H. Vorster, 2004. The effect of red palm olein and refined palm olein on lipids and haemostatic factors in hyperfibrinogenaemic subjects. Throm. Res., 113: 13-25.
PubMed  |  

Shad, M.A., A.T. Iqbal and M. Tayyabe, 2002. Serum lipid profile: The effects of dietary pufa and mufa an experimental animal study. Prof. Med. J., 9: 116-120.
Direct Link  |  

Snedecor, G.R. and R.G. Cochran, 1980. Statistical Methods. 7th Edn., Iwoa State University Press, Ames, IA. USA., pp: 39-63.

Trichopoulou, A., A. Naska and T. Costacou, 2002. Dafen III group disparities in food habits across Europe. Proc. Nutr. Soc., 61: 553-558.

Wardlaw, G.M. and J.T. Snook, 1990. Effect of diets high in butter, corn oil, or high-oleic acid sunflower oil on serum lipids and apolipoproteins in men. Am. J. Clin. Nutr., 51: 815-821.
PubMed  |  

Watkins, B.A. and M.F. Seifert, 2000. Conjugated linoleic acid and bone biology. J. Am. Coll. Nutr., 19: 478-486.
Direct Link  |  

Watkins, B.A., C.L. Shen, J.P. McMurtry, H. Xu, S.D. Bain and K.G. Allen, 1997. Dietary lipids modulate bone prostaglandin E2 production, insulin-like growth factor-1 concentrations and formation rate in chicks. J. Nutr., 127: 1084-1091.
Direct Link  |  

Watkins, B.A., C.L. Shen, K.G. Allen and M.F. Seifert, 1996. Dietary (n-3) and (n-6) polyunsaturates and acetylsalicylic acid alter ex vivo PGE2 biosynthesis, tissue IGF-I levels and bone morphometry in chicks. J. Bone. Miner. Res., 11: 1321-1332.
PubMed  |  

Watkins, B.A., Y. Li, K.G. Allen, W.E. Hoffman and M.F. Seifert, 2000. Dietary ratio of (n-6), (n-3) polyunsaturated fatty acids alter the fatty acid composition of bone compartments and biomarkers of bone formation in rats. J. Nutr., 130: 2274-2284.
Direct Link  |  

Yang, L.C., J.B. Wu, G.H. Ho, S.C. Yang, Y.P. Huang and W.C. Lin, 2008. Effects of poly-γ-glutamic acid on calcium absorption in rats. Biosci. Biotechonol. Biochem., 72: 3084-3090.
CrossRef  |  PubMed  |  Direct Link  |  

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract