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Research Article
 

Antioxidant, Antimicrobial and Antitopoisomerase Screening of the Stem Bark Extracts of Ardisia compressa



Marco Vinicio Ramirez-Mares, Jorge Alberto Sanchez-Burgos and Beatriz Hernandez-Carlos
 
ABSTRACT

The in vitro antioxidant, antimicrobial and antitopoisomerase activities of crude Methanolic (Me) and Hexane (He) extracts of the stem bark of Ardisia compressa (AC) were investigated. Free radical-scavenging activity against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was evaluated, acid ascorbic was used as reference standard. Antimicrobial activity was determined using the agar diffusion method; the bacteria K. pneumoniae, E. coli, S. epidermidis and the fungus C. albicans. Controls employed were chloranphenicol (30 μg) and nystatin (100 units). Topoisomerase inhibition was determined by a clone-forming assay, which uses yeast (S. cerevisiae) strains as a model. Controls included dimethyl sulfoxide (1.66%); campothecin (50 μg/ml), etoposide (100 μg/ml) and sobuzoxane (150 μg/ml). Ascorbic acid was a substantially more powerful antioxidant than the extracts from the stem bark of AC. The Antiradical Efficiency (AE) of ascorbic acid was 249-fold that for He and 8.3-fold that for Me. Only the growth of Klebsiella pneumoniae was inhibited by Me (MIC = 1.875 mg/ml) and He (MIC = 0.9375 mg/ml), while the growth of the other strains were not inhibited. He extract not showed antitopoisomerase activity. Me extract showed antitopoisomerase activity (-15.3%). Open column chromatography of the methanolic extract was conducted and fourteen fractions were collected and tested. Fractions VI (-21.55%), X (-42.49%), XI (-97.68%), XII (-52.34%) and XIII (-35.97%) showed antitopoisomerase I activity and fraction XIV (-79%) antitopoisomerase II (poison) activity. These results suggest that Me extract of the stem bark of A. compressa could be promising in its potential usefulness for treatment of cancer and deserves further investigation.

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  How to cite this article:

Marco Vinicio Ramirez-Mares, Jorge Alberto Sanchez-Burgos and Beatriz Hernandez-Carlos, 2010. Antioxidant, Antimicrobial and Antitopoisomerase Screening of the Stem Bark Extracts of Ardisia compressa. Pakistan Journal of Nutrition, 9: 307-313.

DOI: 10.3923/pjn.2010.307.313

URL: https://scialert.net/abstract/?doi=pjn.2010.307.313

REFERENCES
Adhami, V.M. and H. Mukhtar, 2007. Anti-oxidants from green tea and pomegranate for chemoprevention of prostate cancer. Mol. Biotechnol., 37: 52-57.
CrossRef  |  

Brand-Williams, W., M.E. Cuvelier and C. Berset, 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol., 28: 25-30.
CrossRef  |  Direct Link  |  

Burgess, J.G., E.M. Jordan, M. Bregu, A. Mearns-Spragg and K.G. Boyd, 1999. Microbial antagonism: A neglected avenue of natural products research. J. Biotechnol., 70: 27-32.
CrossRef  |  

Chitra, M., E. Sukumar, V. Suja and C.S. Devi, 1994. Antitumor, antiinflammatory and analgesic property of Embelin, a plant product. Chemotherapy, 40: 109-113.
PubMed  |  Direct Link  |  

Cho, K.H., J.M. Pezzuto, J.L. Bolton, V.E. Steele, G.J. Kelloff, S.K. Lee and A. Constantinou, 2000. Selection of cancer chemopreventive agents based on inhibition of topoisomerase II activity. Eur. J. Cancer, 36: 2146-2156.
Direct Link  |  

De Mejia, E.G., M.V. Ramirez-Mares, E. Arce-Popoca, M. Wallig and S. Villa-Trevino, 2004. Inhibition of liver carcinogenesis in Wistar rats by consumption of an aqueous extract from leaves of Ardisia compressa. Food Chem. Toxicol., 42: 509-516.
CrossRef  |  

De Mejia, E.G., S. Chandra, M. Ramirez-Mares and W. Wang, 2006. Catalytic inhibition of human DNA topoisomerase by phenolic compounds in Ardisia compressa extracts and their effect on human colon cancer cells. Food Chem. Toxicol., 44: 1191-1203.
PubMed  |  Direct Link  |  

Gonzalez de Mejia, E.G., M.V. Ramirez-Mares and M.G. Nair, 2002. Topoisomerase I and II enzyme inhibitory aqueous extract of Ardisia compressa and ardisin protect against benomyl oxidation of hepatocytes. J. Agric. Food Chem., 50: 7714-7719.
PubMed  |  Direct Link  |  

Greenwood, D., 1989. Antibiotic Sensitivity Testing. In: Antimicrobial Chemotherapy, Greenwood, D. (Ed.). Oxford University Press, New York, pp: 91-100.

Hsieh, C.Y and S.T. Chang, 2010. Antioxidant activities and xanthine oxidase inhibitory effects of phenolic phytochemicals from Acacia confusa twigs and branches. J. Agric. Food Chem., 58: 1578-1583.
CrossRef  |  Direct Link  |  

Jannatipour, M., Y.X. Liu and J.L. Nitiss, 1993. The top 2-5 mutant of yeast topoisomerase II encodes an enzyme resistant to etoposide and amsacrine. J. Biol. Chem., 268: 18586-18592.

Kang, Y.H., W.H. Kim, M.K. Park and B.H. Han, 2001. Antimetastatic and antitumor effects of benzoquinonoid AC7-1 from Ardisia crispa. Int. J. Cancer, 93: 736-740.
PubMed  |  Direct Link  |  

Kobayashi, H. and E. de Mejia, 2005. The genus Ardisia: A novel source of health-promoting compounds and phytopharmaceuticals. J. Ethnopharmacol., 96: 347-354.
CrossRef  |  

Mann, C.D., C.P. Neal, G. Garcea, M.M. Manson, A.R. Dennison and D.P. Berry, 2009. Phytochemicals as potential chemopreventive and chemotherapeutic agents in hepatocarcinogenesis. Eur. J. Cancer Prev., 18: 13-25.
PubMed  |  Direct Link  |  

Nikaido, H. and M. Vaara, 1985. Molecular basis of bacterial outer membrane permeability. Microbiol. Rev., 49: 1-32.
PubMed  |  

Nikolovska-Coleska, Z., L. Xu, Z. Hu, Y. Tomita and P. Li et al., 2004. Discovery of embelin as a cell-permeable, small molecular weight inhibitor of XIAP through structure-based computational screening of traditional herbal medicine three-dimensional structure database. J. Med. Chem., 47: 2430-2440.
PubMed  |  Direct Link  |  

Nitiss, J.L. and J.C. Wang, 1988. DNA topoisomerase-targeting antitumor drugs can be studied in yeast. Proc. Natl. Acad. Sci., 85: 7501-7505.
Direct Link  |  

Nitiss, J.L. and K.C. Nitiss, 2001. Yeast systems for demonstrating the targets of antitopoisomerase II agents. Methods Mol. Biol., 95: 315-327.
CrossRef  |  

Nostro, A., M.P. Germano, V. D'Angelo, A. Marino and M.A. Cannatelli, 2000. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Applied Microbial., 30: 379-384.
CrossRef  |  PubMed  |  Direct Link  |  

Ralph, S.J., S. Rodriguez-Enriquez, J. Neuzil, E. Saavedra and R. Moreno-Sanchez, 2010. The causes of cancer revisited: Mitochondrial malignancy and ROS-induced oncogenic transformation - Why mitochondria are targets for cancer therapy. Mol. Aspects Med., 31: 145-170.
PubMed  |  Direct Link  |  

Ramirez-Mares, M.V., S. Chandra and E.G. de Mejia, 2004. In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols. Mutat. Res., 554: 53-65.
PubMed  |  Direct Link  |  

Ramirez-Mares, M.V., S. Fatell, S. Villa-Trevino and E.G. de Mejia, 1999. Protection of extract from leaves of Ardisia compressa against benomyl-induced cytotoxicity and genotoxicity in cultured rat hepatocytes. Toxicol. In vitro, 13: 889-896.
CrossRef  |  

Ramirez-Marez, M.V. and E.G. de Mejia, 2003. Comparative study of the antioxidant effect of ardisin and epigalloca techin gallate in rat hepatocytes exposed to benomyl or nitropyrene. Food Chem. Toxicol., 41: 1527-1535.
PubMed  |  

Schweizer, F., 2009. Cationic amphiphilic peptides with cancer-selective toxicity. Eur. J. Pharmacol., 625: 190-194.
CrossRef  |  PubMed  |  Direct Link  |  

Shureiqi, I., P. Reddy and D.E. Brenner, 2000. Chemoprevention: General perspective. Crit. Rev. Oncol. Hematol., 33: 157-167.
Direct Link  |  

Siddhuraju, P. and K. Becker, 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J. Agric. Food Chem., 51: 2144-2155.
CrossRef  |  PubMed  |  Direct Link  |  

Siddiqui, I.A., M. Saleem, Y.M. Adhami, M. Asim and H. Mukhtar, 2007. Tea beverage in chemoprevention and chemotherapy of prostate cancer. Acta Pharmacol. Sin., 28: 1392-1408.
PubMed  |  

Sumino, M., T. Sekine, N. Ruangrungsi, K. Igarashi and F. Ikegami, 2002. Ardisiphenols and other antioxidant principles from the fruits of Ardisia colorata. Chem. Pharm. Bull., 50: 1484-1487.
PubMed  |  Direct Link  |  

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