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Research Article
 

Total Antioxidant Capacity, Nutritional Composition and Inhibitory Activity of Unripe Plantain (Musa paradisiacae) on Oxidative Stress in Alloxan Induced Diabetic Rabbits



C.O. Eleazu, P.N. Okafor and Ikpeama Ahamefuna
 
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ABSTRACT

The antioxidant composition of unripe plantain and its free radical scavenging activity on alloxan induced diabetic rabbits and on DPPH radical was investigated. 10 male rabbits weighing between 1.58 and 1.88 kg were used for this study. Diabetes was induced in the experimental rabbits with alloxan (35 mg/kg body weight. ip). Group 1 rabbits served as the control groups and they received normal rabbit feeds. Group 2 rabbits were the animals of group 1 which were made diabetic by the injection of alloxan and they also received normal rabbit feeds while group 3 rabbits (test groups) were the animals of group 2 which were later fed with unripe plantain at a dosage of 25 kg/kg body weight/day for 4 weeks. The duration of the experiment was 7 weeks and the weights of the animals in each group were recorded daily throughout the experiment while the blood glucose levels, malonaldehyde, catalase and glutathione were recorded on a 2 weeks interval. The results show that the diabetic rabbits placed on unripe plantain diet had an increase in their body weights, glutathione and catalase levels but a decrease in malonaldehyde and blood glucose levels after 4 weeks of unripe plantain intake when compared with the control (p<0.05). Correlation analysis carried out revealed that glutathione correlated negatively with malonaldehyde and glucose (r = -0.77 and -0.89), but positively with catalase and body weight (r = 0.60 and 0.70). Malonaldehyde correlated negatively with catalase and body weight (r = -0.44 and -0.72) but positively with glucose (r = 0.86). The antioxidant composition of the methanolic extracts of the unripe plantain flour as determined by the quantities of peroxidase and quercetin present was 52±0.00% peroxidase and 5.32 ug/ml quercetin while its free radical scavenging activity on DPPH radical was 78.57±0.00%. Analysis of the proximate and phytochemical composition of the unripe plantain flour showed that it contained 3.16±0.04% protein, 0.21±0.003% lipid, 52±2.82% moisture, 5.5±0.42% ash, 1.58±0.04% tannin, 1.82±0.05% saponin, 1.37±0.05% alkaloid and 0.98±0.00% flavonoid. These findings suggest that raised blood glucose level in diabetics could deplete cells of their antioxidant status by decreasing the glutathione and red cell catalase levels leading to an increased malonaldehyde level which is a marker of oxidative stress. In addition, unripe plantain intake by a diabetic could exert a free radical scavenging activity by restoring the altered antioxidant status since itself could serve as a natural source of antioxidants.

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  How to cite this article:

C.O. Eleazu, P.N. Okafor and Ikpeama Ahamefuna, 2010. Total Antioxidant Capacity, Nutritional Composition and Inhibitory Activity of Unripe Plantain (Musa paradisiacae) on Oxidative Stress in Alloxan Induced Diabetic Rabbits. Pakistan Journal of Nutrition, 9: 1052-1057.

DOI: 10.3923/pjn.2010.1052.1057

URL: https://scialert.net/abstract/?doi=pjn.2010.1052.1057

REFERENCES
1:  Agunbiade, S.O., J.O. Olanlokun and O.A. Olaofe, 2006. Quality of chips produced from rehydrated dehydrated plantain and banana. Pak. J. Nutr., 5: 471-473.
CrossRef  |  Direct Link  |  

2:  Blois, M.S., 1985. Antioxidant determination by use of stable free radicals. Nature, 29: 1199-1200.

3:  Brakohiapa, L.A., I.K. Quaya, A.G. Amoah, E.K. Harrison, D.O. Kennedy, Y. Kido and E. Ofei, 2001. Noguchi Memorial Institute for Medical Research. University of Ghana, London, pp: 220-221.

4:  Ceriello, A., N. Bortolotti, E. Motz, A. Crescentini and S. Lizzio et al., 1998. Meal generated oxidative stress in type 2 diabetic patients. Diabetes Care, 21: 1529-1533.
CrossRef  |  

5:  Chhanda, M., M. Rajkumar and G. Debidas, 2006. Comparative study on the antihyperglycemic and antihyperlipidemic effects of separate and composite extracts of seed of Euglena jambolana and root of Musa paradisiaca in streptozotocine induced diabetic male albino rats. J. Pharmacol. Therapeut., 85: 27-33.

6:  Cohen, G., D. Dembiec and J. Marcus, 1970. Measurement of catalase activity in tissue extracts. Ann. Biochem., 34: 30-38.
CrossRef  |  PubMed  |  Direct Link  |  

7:  Dohi, T., K. Kawamura, K. Morita, H. Okamola and A. Tsiyimoto, 1988. Alterations of the plasma selenium concentrations and the activities of tissue peroxide metabolism enzymes in streptozotocin-induced diabetic rats. Horn. Metab. Res., 20: 671-675.
CrossRef  |  PubMed  |  

8:  Dominquez, C., E. Ruiz, M. Gussinye and A.C. Carrascosa, 1998. Oxidative stress at onset and early stages of type1 diabetes in children and adolescents. Diabetes Care, 21: 1736-1742.

9:  Geetha, G.F., A.M. Ferraris, M. Rolfo, R. Mangerini, S. Arena and H.N. Kirkman, 1996. Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocyte blood. Am. J. Clin. Nutr., 27: 1026-1034.

10:  Gomathy, R., I. Vijayalekshmi and P.A.C. Kurup, 1990. Hypoglycemic action of the pectin present in the inflorescence stalk of plantain (Musa Sapentum)-Mechanism of action. J. Biosci., 15: 297-303.

11:  Harbone, J.B., 1973. Comparative Biochemistry of the Flavonoids. Academic Press, New York, pp: 221-222.

12:  Heath, R.L. and L. Packer, 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125: 189-198.
CrossRef  |  PubMed  |  Direct Link  |  

13:  Hsu, C.L., W. Chen, Y.M. Weng and C.Y. Tseng, 2003. Chemical composition, physical properties and antioxidant activities of yam flours as affected by different drying methods. Food Chem., 83: 85-92.
CrossRef  |  

14:  Ketiku, A.O., 1973. Chemical composition of unripe (green) and ripe plantain (Musa paradisiaca). J. Sci. Food Agric., 24: 703-707.
CrossRef  |  

15:  King, H., R.E. Aubert and W.H. Herman, 1998. Global burden of diabetes, 1995-2025: Prevalence, numerical estimates and projections. Diabetes Care, 21: 1414-1431.
CrossRef  |  PubMed  |  Direct Link  |  

16:  Kirakosyan, A., E. Seymour, O.B. Kaufman, S. Warber, S. Bolling and S.C. Chang, 2003. Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (Hawthorn) subjected to drought and cold stress. J. Agric. Food Chem., 51: 3973-3976.
Direct Link  |  

17:  Nourooz-Zadeh, J., A. Rahimi, J. Tajaddini-Sarmadi, H. Tritschler, P. Rosen, B. Halliwel and D.J. Betteridge, 1997. Relationships between plasma measures of oxidative stress and metabolic control in NIDDM. Diabetologica, 40: 647-653.
CrossRef  |  Direct Link  |  

18:  Piedrola, G., E. Novo, F. Escober and R. Garcia, 2001. White blood count and insulin resistance in patients with coronary artery disease. Ann. Endocrinol., 62: 7-10.
PubMed  |  

19:  Polidori, M.C., P. Mecocci, W. Stahl, B. Parente and P. Cecehelti et al., 2000. Plasma levels of lipophilic antioxidants in very old patients with type 2 diabetes. Diabet Metab. Res. Rev., 16: 15-19.

20:  Price, K.R., I.T. Johnson, G.R. Fenwick and M.R. Malinow, 1987. The chemistry and biological significance of saponins in foods and feedingstuffs. Crit. Rev. Food Sci. Nutr., 26: 27-135.
CrossRef  |  PubMed  |  Direct Link  |  

21:  Rao, B.K., P.R. Sudarshan, M.D. Rajasekhar, N. Nagaraju and C.A. Rao, 2003. Antidiabetic activity of Terminaliapallida fruit in alloxan induced diabetic rats. J. Ethnopharmacol., 85: 169-172.
CrossRef  |  Direct Link  |  

22:  Robbins, R.J., 2003. Phenolic acids in foods:  An overview of analytical methodology. J. Agric. Food Chem., 51: 2866-2887.
CrossRef  |  PubMed  |  Direct Link  |  

23:  Shin, S.H., 1998. Oxidative Stress and Diabetic Vascular Complications: Recent Advances in Pathogenesis and Management of Diabetes Mellitus. 1st Edn., Elsvier Science Co., Singapore, pp: 3-8.

24:  Tagami, S., T. Kondo, K. Yoshidfa, J. Hirokaw, Y. Ohsuka and Y. Kaweasmi, 1992. Effect of insulin on impaired antioxidant activities on aortic endothelial cells from diabetic rabbits. Metabolism, 41: 1053-1058.
PubMed  |  

25:  Udoh, A.E., I. Ntu, O. Essien and M. Ndon, 2007. Red cell catalase activity in diabetics. Pak. J. Nutr., 6: 511-515.
CrossRef  |  Direct Link  |  

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