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Nutrients Intake, Performance and Nitrogen Balance of West African Dwarf Sheep Fed Graded Levels of Toasted Enterolobium cyclocarpum Seeds as Supplement to Panicum maximum



O.J. Idowu, O.M. Arigbede, P.A. Dele, J.A. Olanite, O.O Adelusi, V.O.A. Ojo and A.S. Sunmola
 
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ABSTRACT

A study was conducted to assess the nutritive value of Enterolobium cyclocarpum seeds as supplementary feed for ruminant animals during the dry season when grasses are either not available or of low quality. Matured fruits of E. cyclocarpum were collected, toasted, peeled and then used for the trial. Thirty two West African Dwarf (WAD) sheep aged between 12±2 months with an average body weight of 10±2 kg were used in assessing the nutritive value of graded levels of toasted Enterolobium cyclocarpum seed in a concentrate diets as supplement to Panicum maximum basal diet. The percent compositions of the experimental diets were toasted E. cyclocarpum seeds at various levels of inclusion (0, 10, 20 and 30%) for diets 1, 2, 3 and 4. respectively. The diets (1-4) were consecutively fed to each animal at 50 g kg-1 b.wt. for 12 weeks in a completely randomized design. Parameters taken were weekly body weights, daily feed intake, nutrient utilization and nitrogen balance status for each animal. Diet 2 had the highest significant (p<0.05) nutrients intake being 871.88, 137.13, 147.59, 33.26 and 69.86 g day-1 for DM, CP, CF, EE and ASH respectively. The Dry Matter Digestibility (DMD) coefficients decreased significantly (p<0.05) with increased inclusion levels of toasted E. cyclocarpum seeds supplementation. Sheep fed diet 4 had the lowest feed conversion ratio (8.61) and the highest daily average gain of 58.93 g. However the animals fed Diet 2 had the highest nitrogen retension and converted their feed to flesh.

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  How to cite this article:

O.J. Idowu, O.M. Arigbede, P.A. Dele, J.A. Olanite, O.O Adelusi, V.O.A. Ojo and A.S. Sunmola, 2013. Nutrients Intake, Performance and Nitrogen Balance of West African Dwarf Sheep Fed Graded Levels of Toasted Enterolobium cyclocarpum Seeds as Supplement to Panicum maximum. Pakistan Journal of Biological Sciences, 16: 1806-1810.

DOI: 10.3923/pjbs.2013.1806.1810

URL: https://scialert.net/abstract/?doi=pjbs.2013.1806.1810
 
Received: January 15, 2013; Accepted: March 01, 2013; Published: May 16, 2013



INTRODUCTION

Globally, there are problems related to ruminant animal feeding in terms of matching the available feed resources with their nutrient requirements and this has been a major concern of researchers, part of their effort to finding solution to this problem is the use of tropical browse and shrub legumes plant. Tropical trees and shrubs have great potentials to serve as feed resources for ruminant animals managed by small holder livestock farmers. They are less susceptible to climatic fluctuations (Dzowela et al., 1995) and they provide green foliage of high digestibility and protein contents when most forage resources are low in nitrogen (Preston and Leng, 1987), but they have been found to contain antinutritional factors which tends to affect both their intake and digestibility (Babayemi et al., 2006). Enterolobium cyclocarpum is a legume tree which belongs to the family mimosadeae (Janzen, 1981). The legume is easily established and fast growing to maturity over a short period of time than the most common legume plants in Nigeria and can be used in intensive feed garden in some parts of Nigeria (Ezenwa, 1998). Its agronomic potential has been exploited. Also as a leguminous multipurpose plant, it has the potential of fixing atmospheric nitrogen into the soil and can also be exploited for feeding of ruminant animals. Navas-Camacho et al. (1993) also reported feeding the leaves of E. cyclocarpum to sheep but in the southwest of Nigeria, Enterolobium cyclocarpum foliage has not been accepted by sheep, goats and cattle possibly due to the presence of antinutritional factor. Most of the leguminous browse and shrubs produces seed and fruit which have been found containing higher crude protein and other nutrients. Studies have shown that the seeds produce higher volatile fatty acids on degradation by rumen microbial organisms which tend to be beneficial to ruminant animals (Babayemi et al., 2004a) showing the potential of browse tree seeds in livestock production. E. cyclocarpum (Ear pod tree) is a medium sized to a large tree growing to 25-30 m tall and with a trunk diameter up to 3.5 m. It is high in crude protein and other nutrients (Babayemi et al., 2004a) and the seeds contain an anti-nutritional factor. It should be noted that potential of these crop seeds has not been fully exploited for ruminant feeding. The aim of the present study therefore was to investigate the effect of feeding graded levels of toasted E. cyclocarpum seeds as a supplement to Panicum maximum on intake, weight gain and nitrogen balance in West African Dwarf (WAD) sheep.

MATERIALS AND METHODS

Experimental site: The study was carried out at the Small Ruminant Unit of the College of Animal Science and Livestock Production (COLANIM) University of Agriculture, Abeokuta (UNAAB), Abeokuta. The site lies within the derived savannah zone on latitude 7°N and longitude 3.5°E. The mean annual rainfall is 1037 mm while the annual temperature range is between 22.50-30.72°C. Relative humidity ranges from 63% in January to 96% in August with yearly average of about 82%.

Fruit collection and Seed toasting: The matured fruits of Enterolobium cyclocarpum were handpicked from the ground. The seeds were manually separated from the pods and then toasted using stove top toasting method. The seeds were heated in a dry, heavy skillet over medium heat for 20-40 min until they’re dark brown in colour and they give off a rich, toasty fragrance. The seeds were stirred frequently for even toasting after which it was removed and allowed to cool; the toasting facilitated the peeling of the coat. The peeled seeds were then used for the trial.

Animals and their feeding: Four concentrate diets were compounded to contain toasted E. cyclocarpum seeds (0, 10, 20, 30% levels), soyabean meal, wheat offal, maize, brewer’s dried grain and salt in the percentages as shown in Table 1. Fresh Panicum maximum was allowed to wilt and then chopped to between 2-4 cm lengths. The concentrate diets were offered to the animals individually based on their body weight (50 g kg-1 b.wt.) at 8:00 h while the chopped P. maximum was offered at 16:00 h.

Thirty two WAD sheep of the same sex of between 10-12 months old weighing an average of 12 kg were used for the trial which lasted for 12 weeks.

Table 1: Composition of experimental diets

The sheep were randomly divided into 4 groups of 8 animals and allocated to the treatment diets in a completely randomized design to evaluate the effects of the inclusion levels of toasted ECS in the concentrate diets on the voluntary intake, weight gain and performance of sheep using Elephant grass as the basal diet. The animals were dewormed and purged of external parasites using Ferbendazole and Pfizona, respectively and housed in individual pens and offered liberal but known quantities of the experimental diets for 14 days preliminary period to adapt the animal to the diets and pen environment, fresh water was made available ad libitum. After 2 weeks adaptation period to the diets, the data collection started.

The sheep were fed according to treatment group. Feed offered to each experimental animal ensured at least a 5% remnant in which both feed offered and refusals were weighed and recorded daily to determine the voluntary feed intake for both the concentrate and basal diets. At the twelfth week of the trial, four animals per treatment were randomly selected and housed individually for digestibility study in a metabolic pen under laid with a wire mesh to aid faecal collection using a flat wooden net. Two days acclimatization period was observed. At 8.00 am total faecal output was collected, total urine output and feed intakes were recorded over seven days period.

Chemical analysis: Feed remnants collected from the animals were dried in the oven at 65°C for 3 days to determine their Dry Matter (DM) contents. The dried samples were then ground with laboratory hammer mill to pass through 1 mm sieve and then preserved for chemical analysis. The proximate composition was determined according to AOAC (2006) while the Fibre fractions were determined according to Van Soest et al. (1991) procedure.

Statistical analysis: The data obtained were subjected to one-way analysis of variance (SAS, 2002) and the levels of significance between treatment means were determined with probability difference of SAS.

RESULTS AND DISCUSSION

Experimental diets: The proximate composition of concentrate diets offered to the sheep is presented in Table 2. The Dry Matter (DM) contents of the diets differ significantly (p<0.05) which increases from Diet 1-4 as percentage of toasted ECS increased in diets. The Dry Matter (DM) content of the concentrate diets were 90.20, 90.23, 90.64 and 90.76%, for Diet 1 to 4, respectively and these were comparable to 90.97% for Guiera senegalensis earlier reported (Muhammad and Garba, 2008).

Table 2: Proximate and fibre composition of experimental diets (%dm) fed to sheep

Table 3: Dry matter and nutrient intake of WAD sheep fed concentrate diets containing graded levels of E. cyclocarpum (g day-1)
Means along the same row with different superscript are different p<0.05, TDMI: Total dry matter intake, TCPI: Total crude protein intake, T.EEI: Total ether extract intake, T.CFI: Total crude fibre intake, T.ASHI: Total ash intake

Increasing levels of ECS in the experimental diets (1-4) significantly influence (p<0.05) dietary compositions of CP and CF. The Crude Fibre (CF) values was least in the control diet (9.37%) and highest in diet 4(14.67%) and it has been reported that maximum dietary CF digestion in the rumen occurs when dietary CP is between 12 and 16% (McAllan, 1991; Sowande, 2004). The CF values across the concentrate diets are quite low which supports the report of FAO (eg. FAO 1983) that concentrates are feeds that contain a high density of nutrients, usually low in crude fibre content (less than 18% of Dry Matter (DM) and high in total digestible nutrients. The EE values of the diets increased with increasing level of toasted ECS, this may be due to the contributory effect of the toasted seeds which tends to increase the lipid contents of the seeds. The ASH values followed the same pattern of CF with the supplementation of the toasted E. cyclocarpum seeds. The Ether Extract (EE), Nitrogen Free Extract (NFE) and Gross Energy (GE) values were however similar (p>0.05) for all diets.

Table 3 shows the DM and nutrient intake of WAD sheep fed the experimental concentrate diets as supplement to Panicum maximum. The Dry Matter Intake (DMI) differs significantly (p<0.05) across the diets. Sheep on the control diet (diet 1) consumed 910.75 g day-1 which was the highest DM intake while the trend reduced with increasing levels of toasted E. cyclocarpum in the other diets. The significantly higher DMI observed for sheep fed the control diet in this study does not agree with the findings of previous studies. Findings have observed that diets low in protein depress intake (Ifut, 1988) and that diets with low energy makes animals consume more feed in order to meet up with their energy requirement (Obioha, 1984).

Table 4: Performance and digestibility of WAD rams fed graded level of concentrate diets containing graded levels of E. Cyclocarpum (g day-1)
Means along the row with different superscripts are different (p<0.05)

Contrary to these facts, diet 1, which had the least CP and the highest energy content of all diets was consumed most Also, this could be attributed to the decreasing palatability of the diets with increasing levels of toasted E. cyclocarpum. The intake of crude protein and crude fibre increased with increasing levels of toasted E. cyclocarpum supplementation. This could be attributed to the fact that the toasted E. cyclocarpum seeds is rich in protein and also the inclusion levels tend to increase the fibre content and this subsequently decrease the dry matter intake, ECS has been reported to contain high Crude Protein (CP) and Crude Fibre (CF) (Babayemi et al., 2006). Increasing levels of ECS in diets 2-4 increased the CP content and CF content, this may explain why the ECS diets were consumed less in relation to the control in this study. Meanwhile, among the ECS diets, diet 2 may have yielded the best-synchronized release of nitrogen and carbohydrate (Silva and Orskov, 1985) in the rumen required for microbial protein synthesis. This may have influenced the observed superior DMI for sheep on diet 2 and the observed poor but relatively higher nitrogen utilization value for animals fed same diet (2). The significantly higher EEI observed in sheep fed the diet 4 may be due to the increased level of toasted ECS which tends to decrease the digestibility of nutrients at this level of supplementation.

Performance: The data on performance characteristics of West African Dwarf (WAD) sheep offered diets containing graded levels of toasted E. cyclocarpum seeds as supplement for Panicum maximum is shown in Table 4. It was observed that the weight gain values decreased with increasing level of toasted E .cyclocarpum seed inclusion in the diet as the highest daily weight gain value (58.93 g) occurred at 10% level of supplementation and the least daily weight gain value (47.62 g) observed at 30% level of supplementation.

Table 5: Nitrogen Utilization of WAD rams fed graded level of concentrate diets containing graded levels of E. cyclocarpum (g day-1)
Means along the row with different superscripts are different (p<0.05)

The reduced feed consumption, weights and feed/BWgain as the level of ECS in the diets increased, demonstrate the negative effect of the toasted seeds of E. cyclocarpum on the sheeps’ performance. Similar results have been reported by Iyayi et al. (2006) when they fed diets containing E. cyclocarpum and M. pruriens seed flour in place of soybean meals to broiler. The daily weight gain was not significantly (p>0.05) affected by the inclusion of toasted E. cyclocarpum seed in the diets, may be as a result of increased ether extract produced from the increasing level of supplementation of toasted E. cyclocarpum seeds.

The reduced digestibility of CP in the diets was implicated in the poor performance of animals on these diets. The higher digestibility of CP in the diet 2 is probably an indication that at low levels of replacement of toasted ECS by the sheep, ingested amounts of the major antinutrients in E. cyclocarpum e.g. Saponin, Phenols and steroids which tends to deter the activities of bacteria in the rumen and the extent to which the toasted seed will be fermented in the rumen are not enough to affect CP digestibility (Babayemi et al., 2004b).

Nitrogen utilization: The nitrogen utilization of sheep offered diets containing graded levels of toasted E. cyclocarpum seeds as supplement for Panicum maximum is indicated in Table 5. Nitrogen intake (g day-1) from diet (2) compared fairly well with the value derived for the control diet but differed significantly (p<0.05) from those of diets 3 and 4. The implication of this is that addition of toasted ECS in sheep diets at levels below 10% will not improve nitrogen intake. At higher levels however, nitrogen intake will improve but would be optimal at 20% ECS inclusion. ECS is a good source of nitrogen, which can be used to improve low protein-high energy feedstuffs.

Faecal nitrogen did not differ significantly (p>0.05) among the diets. The values obtained for sheep fed toasted ECS diets (2, 3, 4) were similar (p<0.05) and did not differ significantly (p>0.05) from those of sheep fed the control diet. This observation runs contrary to the findings of Black et al. (1973), who reported that faecal nitrogen was not affected by nitrogen intake.

Urinary nitrogen (g day-1) values were higher for sheep fed toasted ECS in which diet 2 had the highest value, the differences were however, significantly different (p<0.05), when compared with the control. The higher value recorded for diet 2 may be due to the fact it is at this level that the best-synchronized release of nitrogen and carbohydrate (Silva and Orskov, 1985) in the rumen required for microbial protein synthesis can be accomplished since Urinary nitrogen is a function of nitrogen ingested, the more the N intake, the more the quantity excreted in the urine (Ibeawuchi et al., 1993).

The sheep fed the graded levels of toasted ECS possessed a positive nitrogen balance required for body growth and maintenance with animals on diet 2 having the highest value (87.76%), followed by those on the control diet(86.37%) and diet 4 having the least value (85.37%). The positive nitrogen balance observed in all the animals suggested that nitrogen absorbed was well tolerated and utilized by the animals.

CONCLUSION

The results obtained from this study showed that toasted E. cyclocarpum seed at 10% level of supplementation is of good nutritional profile as a source of dry matter, crude protein, crude fibre, as well as nitrogen retention and feed/gain ratio when fed to WAD sheep without any deleterious effect on nutrient intake and digestion. It is therefore concluded that inclusion of toasted E. cyclocarpum seeds in small ruminant diets especially during feed scarcity will help to augment their nutrient intake and reduce the feed stress of the dry season therefore enhancing their performance and productivity.

REFERENCES
1:  AOAC., 2006. Official Methods of Analysis. 18th Edn., Association of Official Analytical Chemists Inc., Arlington, TX., USA.

2:  Babayemi, O.J., D. Demeyer and V. Fievez, 2004. Nutritive value and qualitative assessment of secondary compounds in seeds of eight tropical browse, shrub and pulse legumes. Commun. Agric. Applied Biol. Sci., 69: 103-110.
PubMed  |  

3:  Babayemi, O.J., D. Demeyer and V. Fievez, 2004. In vitro rumen fermentation of tropical browse seeds in relation to their content of secondary metabolites. J. Anim. Feed Sci., 1: 31-34.
Direct Link  |  

4:  Babayemi, O.J., F.T. Ajayi, A.A. Taiwo, M.A. Bamikole and A.K. Fajimi, 2006. Performance of West African dwarf goats fed Panicum maximum and concentrate diets supplemented with Lablab (Lablab purpureus), Leucaena (Leucaena leucocephala) and Gliricidia (Gliricidia sepium) foliage. Nig. J. Anim. Prod., 33: 102-111.

5:  Black, J.L., G.R. Pearce and D.E. Tribe, 1973. Protein supplement of growing lambs. Br. J. Nutr., 30: 45-60.

6:  Dzowela, B.H., L. Hove and P.L. Mafongoya, 1995. Effect of drying method on chemical composition and in vitro digestibility of multipurpose tree and shrub fodder. Tropical Grassland, 29: 263-269S.
Direct Link  |  

7:  Ezenwa, I., 1998. Preliminary evaluation of the suitability of Enterolobium cyclocarpum for use in intensive feed garden in Southwestern Nigeria. Agrofor. Syst., 44: 13-19.
CrossRef  |  

8:  FAO, 1983. Production Year Book. Food and Agricultural Organization, Rome, Italy.

9:  Muhammad, I.R. and Y. Garba, 2008. Sabara (Guiera senegalensis) as browse and a potential milk enhancer in ruminants in the semi-arid environment. Res. J. Anim. Sci., 2: 123-127.
Direct Link  |  

10:  Ibeawuchi, J.A., A. Danjuma and T. Oguntona, 1993. The value of dried poultry waste as protein supplement for growing bornu white goats. Discovery Innovat., 5: 63-68.

11:  Ifut, O.J., 1988. The Potential of Cassava Peels for Feeding Goats in Nigeira. In: Cassava as Livestock Feed in Africa, Hahn, S.K., L. Reynolds and G.N. Egbunike (Eds.). International Livestock Centre for Agriculture, Ibadan, Nigeria, pp: 72-81.

12:  Iyayi, E.A., H. Kluth and M. Rodehutscord, 2006. Precaecal crude protein digestibility, organs relative weight and performance in broilers fed diets containing Enterolobium cyclocarpum and Mucuna pruriens seed flour in place of soybean meal. Archiv Fur Geflugelkunde, 70: 161-167.
Direct Link  |  

13:  Janzen, D.H., 1981. Guanacaste tree seed-swallowing by Costa Rican Range horses. Ecology, 62: 587-592.
CrossRef  |  Direct Link  |  

14:  McAllan, A.B., 1991. Sources of Nitrogen Supplements to Maximum Cereal Crop Residue Digestion in the Rumen. In: Production and Utilization of Lignocellulosics, Galletti, G.C. (Ed.). Elsevier Science Publisher Ltd., UK., ISBN-13: 9781851666492, pp: 375-385.

15:  Navas-Camacho, A., M.A. Laredo, A. Cuesta, H. Anzola and J.C. Leon, 1993. Effect of supplementation with a tree legume forage on rumen function. Livest. Res. Rural Dev., Vol. 5.

16:  Obioha, F.C., 1984. Potential grain substitutes for monogastric animal feeding in tropics. Proceedings of the 3rd Tropaq Animal Nutrition Course, University of Edinburgh, Scotland.

17:  Preston, T.R. and R.A. Leng, 1987. Matching Ruminant Production Systems with Available Resources in the Tropics and Sub Tropics. International Colour Productions Stanthorpe, Penambul Books, Queensland, Austrailia, Pages: 956.

18:  SAS, 2002. Users Guide Statistics. SAS Institute, Cary, NC.

19:  Silva, A. and E.R. Orskov, 1985. Effect of unmolassed sugar beet pulp on rate of straw degradation in the rumen of sheep given barley straw. Proc. Nutr. Soc. UK., 44: 50-50.

20:  Sowande, O.J., 2004. Response of West African Dwarf sheep to dry season feeds, based on preserved elephant grass layer's dropping and cassava peels. Ph.D. Thesis, Department of Animal Production and Health, University of Agriculture, Abeokuta, Nigeria.

21:  Van Soest, P.J., J.B. Robertson and B.A. Lewis, 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597.
CrossRef  |  PubMed  |  Direct Link  |  

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