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Research Article
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Determination of Yield Stability in Advanced Potato Cultivars as Affected by Water Deficit and Potassium humate in Ardabil region, Iran |
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Davoud Hassanpanah,
Elshad Gurbanov,
Aladdin Gadimov
and
Reza Shahriari
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ABSTRACT
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This experiment was done on the three potato cultivars [Agria (susceptible),
Satina (semi-tolerant) and Ceaser (tolerant to water deficit)] and three
irrigation treatments (after 30 mm evaporation from basin class A, after
60 mm evaporation+spraying by Potassium Humate, and after 60 mm evaporation
from basin class A) for two locations in 2007. Experimental design was
Split Plot with three replications. Potassium Humate spraying (250 mL
ha-1) were done in three stages of emergence, before tuberization
and during tuberization period. Combined analysis of variance showed that
there were significant differences between locations, cultivars, irrigation
treatments and locationxcultivars interaction as effect on tuber yield.
Comparison of means for irrigation treatments showed that spraying by
Potassium Humate in stress condition induced increasing of tuber yield.
Spraying by Potassium Humate in water deficit condition increased tuber
yield up to 11.01 ton ha-1. Ceaser had the highest tuber yield.
It had higher tolerance to water deficit as well. Ceaser had a high potential
in control and severe stress. Decrease in yield of Ceaser after 60 mm
evaporation+spraying by Potassium Humate and after 60 mm evaporation from
basin class A, relative to control (after 30 mm evaporation from basin
class A) was 1.03 and 13.08 ton ha-1 but for Satina was 7.83
and 16.61 ton ha-1, respectively. Satina had the lowest Environmental
Variance, Environmental Variance Coefficient, Finlay and Wilkinson`s and
Eberhart and Russell`s model and was the most stable cultivar. Lin and
Binns parameter showed that Ceaser and Satina were the most stable cultivars.
Results of GMP, STI and MSTI were very considerable and Ceaser and Satina
had a high yield in water stress and control conditions.
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INTRODUCTION
Water stress is a common stress in potato production areas, which
lead to yield and tuber quality decreasing. Because of potato susceptibility
to drought (Foti et al., 1995), preparing sufficient water is very
important for increasing potato quality and quantity. It is very necessary
to study about tolerance of different potato cultivars against water deficit
stress and determination of potato water consumption in Ardabil. There
is a water deficit problem in this region. Climatic changes were occurred
in Ardabil region at the recent years. These changes caused differences
in precipitation dispersion, river flowing and wells water. Therefore,
we have to identify agronomic characters and water need of new potato
cultivars and more improvement of their quality and quantity.
Humates are widespread carbonic matters being formed in the processes
of biological and chemical decomposition of plant and animal residues.
Humates present the complex of high molecular polyfunctional nitrogenic
organic compounds with cyclic structure and specific physical, chemical
and biological characteristics (Lopez-Fernandez et al., 1992).
Humic acid causes to increase yield in watermelon and cabbage and potatoes
(Salman et al., 2005). Humic acid is used to remove or decrease
the negative effects of chemical fertilizers and some chemicals from the
soil. The major effect of humic acid on plant growth has long been reported
(Lee and Bartlette, 1976; Linchan, 1978; Pal and Sengupta, 1985; Hartwigson
and Evans, 2000).
Potassium Humate is an active hormone with natural origin that extracts
from plants and animals remains existed in the bottom of marshes. This
material are formed from N, P, K and microelements namely Mo, Cu, Zn,
B, Co, Mg (Gadimov et al., 2007). Potassium humate causes
increased accumulation of chlorophyll, sugar, amino acids and improves
the efficiency of nitrogen utilization, allowing for reduced fertilizer
rates, the plant`s ability to withstand the stresses of heat, drought,
cold, disease, insect and other types of environmental or cultural pressures
and also increases general plant productivity, in terms of yield, as well
as plant stem strength (Anonymous, 2008). Using of potassium Humate increased
root system, tuber yield, tuber number per plant in potato (Anonymous,
2007) and pea numbers from 14.4 to 52.6 and its weight from 12-36 g in
condition of saline stress with application of 250 mL ha-1
Potassium Humate at 3-6 weeks after planting as spraying and decreased
nitrate amounts in leaves and roots of pea (Gadimov et al., 2007)
and also decreased nitrate accumulation in potato tubers (Hassanpanah
et al., 2007).
To reduce the complications that the GxE interaction creates when selecting
superior genotypes, many attempts have been made to (i) understand the
environmental components causing the GxE interaction (Epinat-Le Signor
et al., 2001), (ii) to examine the GxE interaction biometrically
(Lin et al., 1986; Finlay and Wilkinson, 1963; Yan et al.,
2001) and (iii) to develop selection strategies that involve a stability
parameter (Magari and Kang, 1993).
There are two major stability measures that can be ascribed to the static,
type I stability concept (Lin et al., 1986; Becker and Leon, 1988).
• |
The environmental variance (S2), i.e., the
variance of genotype yields recorded across test or selection environments
(i.e., individual trials). For the genotype i: |
Rij |
= |
Observed genotype yield response in the environment
j (the mij notation may also be appropriate, since values
are averaged across experiment replicates) |
mi |
= |
Genotype mean yield across environments and |
e |
= |
No. of environments |
Greatest stability is S2 = 0. Derived stability measures include
the square root value (S) and its coefficient of variation.
• |
The regression coefficient of genotype yield in individual
environments as a function of the environment mean yield (mj),
adopting Finlay and Wilkinson`s b coefficient (1963). |
The modeled genotype response:
where, ai = intercept value, is analogous to equation reported
for joint regression analysis of adaptation, but genotype responses to
environments (rather than to locations) are of concern here. Greatest
stability is b = 0.
Lin et al. (1986), while ascribing this measure to a type III
stability concept, interpreted it as an indicator of the goodness of fit
of the regression model for describing the stability response.
Lin and Binns (1988) type IV stability concept relates to stability only
in time (i.e., across test years or crop cycles), averaged across test
locations, rather than stability also in space (as implied by stability
analysis across environments).
Finally, Lin and Binns (1991) resulted that parameters type I (Environmental
variance; Environmental variance coefficient) and IV (Lin and Binns within
location variance) were heritable and proper for selection; but parameters
type II (Finlay and Wilkinson) and III (Eberhart and Russell) were not
heritable and proper for selection.
MATERIALS AND METHODS
This experiment was conducted on the three potato cultivars [Agria
(susceptible), Satina (semi-tolerant) and Ceaser (tolerant to water deficit)]
and three irrigation treatments (after 30 mm evaporation from basin class
A, after 60 mm evaporation+spraying by Potassium Humate and after 60 mm
evaporation from basin class A) for two locations in 2007. Experimental
design was Split Plot with three replications. Potassium Humate sprayed
(250 mL ha-1) in the three stages of emergence, before tuberization
and during tuberization period. In the growth period and after harvesting,
some of characters were measured such as main stem number, plant height,
tuber number and weight per plant, total tuber yield, marketable tuber
yield, dry matter percent and marketable tuber number and weight per plant.
Combined analysis of variances were done and comparison of means made
by LSD. Cultivars were evaluated by stability parameters as below:
• |
Environmental variance coefficient |
• |
Finlay and Wilkinson (1963) |
• |
Eberhart and Russell (1966) |
xij |
= |
Mean of genotype i, (i = 1,2,...,p) at environment j,
(j = 1,2,...,q) |
 |
= |
Mean of environment j |
 |
= |
Mean of genotype i in all of environments |
 |
= |
Total mean |
q |
= |
Number of environments |
p |
= |
Number of genotypes |
Si |
= |
Environmental variances |
• |
Lin and Binns within location variance (1988) |
• |
Within location variance coefficient |
Indices used for evaluation of advanced potato cultivars were Fischer
and Maurer stress index (SSI), Fernandez tolerance index (STI), Rosielle
and Hamblin tolerance index (TOL), Baron geometric index (GMP) and Modified
tolerance index (MSTI) as below (Fischer and Maurer, 1978; Frenandez,
1992; Rosielle and Hamblin, 1980; Naderi et al., 1999):
• |
Stress Susceptibility Index (SSI): |
Ysi |
= |
Yield of cultivar in stress condition |
Ypi |
= |
Yield of cultivar in normal condition |
Ys |
= |
Total yield mean in stress condition |
Yp |
= |
Total yield mean in normal condition |
• |
Stress Tolerance Index (STI): |
• |
Geometric Mean Index (GMP): |
• |
Mean Productivity (MP): |
• |
Modified Stress Tolerance Index (MSTI): |
RESULTS AND DISCUSSION
Combined analysis of variance showed that there were significant differences
between locations, cultivars, irrigation treatments and locationxcultivars
interaction on tuber yield. Comparison of means for irrigation treatments
showed that spraying Potassium Humate in stress condition induced increasing
of tuber yield. Spraying by Potassium Humate in water deficit condition
increased tuber yield up to 11.01 ton ha-1. Ceaser had the
highest tuber yield. It had as well as tolerance to water deficit. There
was a high potential in control and severe stress for Ceaser. Decreasing
yield of Ceaser after 60 mm evaporation+ spraying by Potassium Humate
and after 60 mm evaporation from basin class A, relative to control (after
30 mm evaporation from basin class A) was 1.03 and 13.08 ton ha-1
but for Satina was 7.83 and 16.61 ton ha-1, respectively (Table
1).
Because of locationxcultivars interaction significant differences for
tuber yield, the below mentioned parameters were used to estimate the
highest stable cultivars.
Table 1: |
Mean of tuber yield and decreasing yield for potato
cultivars under normal, stress and stress+spraying by Potassium Humate |
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Table 2: |
Estimates of stability parameters for potato cultivars
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Satina had the less variance among the environments and was the most
stable cultivar (Table 2).
Francis and Kannenberg`s (1978) variance of a genotype across environments
(Si2) and the coefficient of variability of each genotype:
These authors used both statistics as stability parameters and proposed
the classification of genotypes based on yield and environmental variance
coefficient. In our experiment Satina with the least environmental variance
coefficient and higher yield than total mean was stable cultivar (Table
2).
These authors had regression means yields of the ith genotype
at the jth environment (Xij) on the environmental
means. According to Finlay and Wilkinson (1963), cultivars with b-values
around 1 have average stability. When average stability is associated
with high yield, varieties are specifically adapted to high-fertile environments
while accompanied low yield, then these varieties were adapted to low-fertile
environments. b-values >1 implies high sensitivity (below average stability)
and adaptability to high-fertile environments. b-values <1 implies
greater resistance to environmental changes and adaptation to low-fertile
environments. From this view of point, Satina with bi = 1 was
the most stable cultivar (Table 2).
Eberhart and Russell (1966) had regression mean yields (Xij)
on environmental indices. They identified an ideal variety as one which
had high mean yield, bi=1 and σij=0. They and
other authors noted that varieties with bi<1 produced yields
which lower than the grand mean of the experiment, while other researchers
found no correlation between yield and b-values. Cultivars like Agria
with bi>1, had lower stability but Satina with bi<1,
had higher stability. Eberhart and Russell (1969) noted that genotypes
are suitable for large fields which increasing environment fertility lead
to yield increasing. Because this, Satina is suitable for Ardabil region
(Table 2).
Lin and Binns (1989) gave types IV of stability. According to them a
variety is stable where:
• |
Its among-environmental variance is small (SS of Years)
|
• |
It has average slope (~1) (SS of YearsxLocations) |
• |
Its standard deviations from regression mean square are small (SS
of YearsxCultivars) |
• |
It has small among-years mean square (SS of YearsxLocationsxYear). |
From this view of point, Ceaser and Satina were the most stable cultivars
(Table 2).
Analyzing of tolerance and sensitivity evaluation indices to environmental
stress conditions showed that efficiency of these indices modify with
genotypes yield variation and aims assessment.
Fischer and Maurer index classified genotypes as tolerant or sensitive.
This index can recognize genotypes as tolerant or sensitive, be regardless
to their yield and have a good efficiency for finding genotypes with resistance
genes. TOL has a conditional efficiency, but after classifying genotypes
to equal TOL, we can select resistant genotypes with MP. Finding equal
TOL in different groups is very hard. With regard to role of TOL and MP,
genotype with high MP may not be exists in the least TOL groups and selecting
superior genotypes may be difficult. Frenandez index uses stress and non-stress
yield and geometric mean. There is a problem and it is geometric equation
of coupling data that have natural difference. Environmental changes in
all of the Iran provinces are visible. MSTI index with calculating KSTI
for suitable and unsuitable conditions is useful for selecting superior
genotypes for each region. MSTI results are very notable.
Yield decreasing in mild drought (SI = 0.09) and severe drought (SI =
0.38) relative to control was 9 and 38%, respectively (Table
3, 4).
MP, GMP, STI and MSTI selected Ceaser; and TOL and SSI selected Agria
as better cultivar under mild stress condition (Table 3).
SSI, STI and MSTI selected Ceaser and Agria; and MP, GMP and TOL selected
Ceaser and Satina as stable cultivars under severe
Table 3: |
Estimates of water stress indices for potato cultivars
under mild stress |
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(SI = 0.09); Yp = Mean of tuber yield in normal condition;
Ys = Mean of tuber yield in stress condition |
Table 4: |
Estimates of water stress indices for potato cultivars
under severe stress |
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(SI = 0.38) |
stress condition (Table 4). Phenologic investigation
of Ceaser and Satina in susceptible periods can lead to understanding
strategic methods in agronomy and breeding practices.
CONCLUSION
Spraying by Potassium Humate in water deficit condition increased
tuber yield. Ceaser and Satina had the highest tuber yield and were tolerant
to water deficit. They were the stable cultivars in Ardabil condition.
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