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Research Article
 

Influence of Auxins Combinations on Accumulation of Reserpine in the Callus of Rauvolfia tetraphylla L.



S. Anitha and B.D. Ranjitha Kumari
 
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ABSTRACT

Reserpine is a monoterpene indole alkaloid used to treat hypertension because of its hypotensive property and psychiatric disorders because of its tranquilizing effect. Protocol has been standardized to enhance the synthesis of reserpine in leaf derived calli of Rauvolfia tetraphylla L. by adjusting the auxins combinations in the medium consisting of MS nutrient salts and B5 vitamins. Auxins such as naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) were used in 1-5 µM concentration along with 9 µM concentration of 2,4 dichlorophenoxy acetic acid (2,4-D), which was found suitable for callus induction. The combination of (2,4-D) with NAA had been proved to accumulate maximum amount of reserpine followed by 2,4-D with IBA. The IAA with 2,4-D combination yielded very less amount of reserpine than the other combinations and 9 µM 2,4-D alone. The results suggest that there may be synergetic effect of NAA with 2,4-D and IBA with 2,4-D for increase in the biomass and reserpine accumulation and antagonistic effect of IAA with 2,4-D for the above said factors in the callus.

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  How to cite this article:

S. Anitha and B.D. Ranjitha Kumari , 2007. Influence of Auxins Combinations on Accumulation of Reserpine in the Callus of Rauvolfia tetraphylla L.. Pakistan Journal of Biological Sciences, 10: 3900-3904.

DOI: 10.3923/pjbs.2007.3900.3904

URL: https://scialert.net/abstract/?doi=pjbs.2007.3900.3904

REFERENCES
1:  Anitha, S. and B.D.R. Kumari, 2006. Stimulation of reserpine biosynthesis in the callus of Rauvolfia tetraphyla L. by precursor feeding. Afr. J. Biotechnol., 5: 659-661.
Direct Link  |  

2:  Anitha, S. and B.D.K. Ranjitha, 2006. Reserpine accumulation in NaCl treated calli of Rauvolfia tetraphylla L. Sci. Asia, 32: 1-3.
Direct Link  |  

3:  Anonymous, 1998. Indian Herbal Pharmacopoeia. In: A Joint Publication of Regional Research Laboratory, Handa, S.S., D. Mundkinajeddu and A.K. Mangal (Eds.), Jammu Tawi, Indian Drug Manufacturers Association, Vol. 1, Mumbai pp: 121-129.

4:  Rajan, G.B., 2001. Biotechnological Advances in Solanum eleagnifolium-night Shade Plant. In: Role of Biotechnology in Medicinal and Aromatic Plants, Irfan A. Khan and Atiya Khanum (Eds.), Vol. 4, Ukkaz Publications, Hyderabad, India, pp: 126-132.

5:  Charlwood, B.V., K.A. Charlwood and J. Molina-Torres, 1995. Accumulation of Secondary Compounds by Organised Plant Cultures. In: Secondary Products from Plant Tissue Culture, Charlwood, B.V. and M.J.C. Rhodes (Eds.), Clarendon Press, Oxford, pp: 167-200.

6:  Dhiman, A.K., 2006. Ayurvedic Drug Plants. Daya Publishing House, New Delhi, pp: 343-345.

7:  Fasil, M. and M. Anis, 2002. Rapid in vitro propagation of Rauvolfia tetraphylla L.-An endangered medicinal plant. Physiol. Mol. Biol. Plants, 8: 295-299.

8:  Holden, P.R., M.A. Holden and M.M. Yeoman, 1988. Variation in the Secondary Metabolism of Cultured Plant Cells. In: Manipulating Secondary Metabolism in Culture, Robins, R.J. and M.J.C. Rhodes (Eds.), Cambridge University Press, Cambridge, pp: 15-29.

9:  Iwase, A., H. Aoyagi, M. Ohme-Takagi and M. Tanaka, 2005. Development of a novel system for producing ajmalicine and serpentine using direct culture of leaves in Catharanthus roseus intact plant. J. BioSci. Bioeng., 99: 208-215.
Direct Link  |  

10:  Lindsey, K., 1988. Prospects for the Genetic Manipulation of Complete Metabolic Pathways. In: Manipulating Secondary Metabolism in Culture, Robins, R.J. and M.J.C. Rhodes (Eds.), Cambridge University Press, Cambridge, pp: 123-136.

11:  Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Planta., 15: 473-497.
CrossRef  |  Direct Link  |  

12:  Narasimhan, S. and G.M. Nair, 2004. Release of berberine and its crystallization in liquid medium of cell suspension cultures of Coscinium fenestratum (Gaertn.) Colebr. Curr. Sci., 86: 1369-1371.
Direct Link  |  

13:  Olivera, A.J.B., K.L.R.F.A. Machado and S.S.L. Kirszenzaft, 2001. Callus culture of Aspidosperma ramiflorum Muell. Arg.: Growth and alkaloid production. Marinza, 23: 609-612.

14:  Pullaiah, T., 2002. Medicinal Plants in India. Vol. 2, Regency Publications, New Delhi, India, pp: 441-443.

15:  Radwanski, E.R. and R.L. Last, 1995. Tryptophan biosynthesis and metabolism. Biochem. Mol. Gen. Plant Cell, 7: 921-934.
PubMed  |  Direct Link  |  

16:  Ralphs, M.H. and D.R. Gardner, 2003. Distribution of norditerpene alkaloids in tall larkspur plant parts through the growing season. J. Chem. Ecol., New York, 29: 2013-2021.
Direct Link  |  

17:  Rhodes, M.J.C., J. Hamill, A.J. Parr, R.J. Robins and N.J. Walton, 1988. Strain Improvement by Screening and Selection Techniques. In: Manipulating Secondary Metabolism in Culture, Robins, R.J. and M.J.C. Rhodes (Eds.), Cambridge University Press, Cambridge, pp: 83-93.

18:  Da Rocha, L.K., A.J.B. de Oliveira, C.A. Mangolin and M.D.F.P. da Silva Machado, 2005. [Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae)]. Acta Scientiarum-Biol. Sci., 27: 37-41, (In Portuguese).
CrossRef  |  Direct Link  |  

19:  Seth, R. and A.K. Mathur, 2005. Selection of 5-methyltryptophan resistant callus lines with improved metabolic flux towards terpenoid indole alkaloid synthesis in Catharanthus roseus. Curr. Sci., 89: 544-548.

20:  Sheludko, Y., I. Gerasimenko, M. Unger, I. Kostenyuk and J. Stockigt, 1999. Induction of alkaloid diversity in hybrid plant cell cultures. Plant Cell Rep., 18: 911-918.
CrossRef  |  

21:  Srividhya, N., B. Poorna Sridevi and P. Sathyanarayana, 1998. Azadirachtin and nimbin content in the in vitro shoots and roots of Azadirachta indica. J. Juss. Indian J. Plant Physiol., 3: 129-134.
Direct Link  |  

22:  Swarup, R. and J.R. Arora, 2000. Plant Tissue Culture from Research to Commercialization: A Decade of Support. Department of Biotechnology, Government of India, New Delhi, pp: 48-49.

23:  Yeh, F.T., W.W. Huang, C.C. Cheng, C. Na and H.S. Tsay, 1994. Tissue culture of Dioscorea doryophora Hance. II. Establishment of suspension culture and the measurement of diosgenin content. Chinese Agron. J., 4: 257-268.

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