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Research Article

Comparison of Antioxidant and Free Radical Scavenging Activities of the Essential Oils from Flowers and Fruits of Otostegia persica Boiss

Fariba Sharififar , Valiolah Mozaffarian and Shirin Moradkhani
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The aerial parts of the endemic plant of Otostegia persica Boiss. in two different stages of flowering and fruiting were hydro-distilled to extract oils in the yields of 0.3 and 0.15% (v/w), respectively. The oils were analyzed by GC and GC/MS. Twenty-eight and thirty-one components were identified, representing 97.59 and 94.61% of the oils, respectively. The main compounds of the essential oil flowers (EOFL) were alpha-pinene (17.21%), 1-octen,3-ol (13.44%) and cubenol (7.27%), whereas diisooctyl phthalate (45%) and hexadecanoic acid (11.07%) were the major constituents of the essential oil of the fruits (EOFR). The oils were screened for their possible antioxidant activities by two complementary test systems, namely DPPH free radical-scavenging and ammonium thiocyanate. In both tested systems, EOFL exerted greater antioxidant and radical scavenging activity. In the first case, EOFL exerted antioxidant activity with an IC50 19.8±1.8 μg mL-1 almost similar to BHA and ascorbic acid (15.2±1.1 and 17.4±1.3), respectively. In the ammonium thiocyanate system, the inhibition rate of oxidation of linoleic acid for EOFL was estimated 93.5±2.8. The higher activity of this oil in comparison to EOFR may be attributed to its high content of monoterpenes, especially oxygenated ones in the oil of the flowers.

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  How to cite this article:

Fariba Sharififar , Valiolah Mozaffarian and Shirin Moradkhani , 2007. Comparison of Antioxidant and Free Radical Scavenging Activities of the Essential Oils from Flowers and Fruits of Otostegia persica Boiss. Pakistan Journal of Biological Sciences, 10: 3895-3899.

DOI: 10.3923/pjbs.2007.3895.3899



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