Subscribe Now Subscribe Today
Research Article

Phytotoxicity of Methyl tert-butyl Ether to Common Bean (Phaseolus vulgaris L.) Plants

Mohamed S. Beltagi
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

The current investigation was conducted to report on the phytotoxicity of methyl tert-butyl ether (MTBE) to common bean (Phaseolus vulgaris L. cv. Nebraska) plants. The two-week-old potted plants were subjected to four weekly soil applications of aqueous MTBE concentrations (0, 1, 10, 25 and 50 ml L-1). The root growth, flower and pod development were more sensitive to MTBE treatments; while, stem growth and photosynthetic pigments were more persistent to the toxicity of MTBE. The total number of protein bands/lane in SDS-PAGE protein profile was reduced by MTBE treatments. Two proteins of molecular weight 53.83 and 30.96 kDa were newly synthesized at the highest concentrations (25 and 50 ml L-1) of MTBE; while the syntheses of other proteins were completely inhibited with varying sensitivity to MTBE concentrations. The toxicity of MTBE concentrations caused progressive collapsing of epidermal and cortical tissues of the plant roots. MTBE is quite toxic to crop plants in contaminated soils of agricultural systems.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Mohamed S. Beltagi , 2007. Phytotoxicity of Methyl tert-butyl Ether to Common Bean (Phaseolus vulgaris L.) Plants. Pakistan Journal of Biological Sciences, 10: 3847-3852.

DOI: 10.3923/pjbs.2007.3847.3852


1:  Abdel-Salam, A.Z.E., H.Z. Hassan, F.M.I. Badaway and W.M. Abdel-Naby, 1996. The mutagenic potentialities of three pesticides on three biological systems. Egypt. J. Genet. Cytl., 22: 109-128.

2:  An, Y.J., D.H. Kampbell and G.W. Sewell, 2002. Water quality at five marinas in Lake Texoma as related to methyl tert-butyl ether (MTBE). Environ. Pollut., 118: 331-336.
CrossRef  |  Direct Link  |  

3:  Beltagi, M.S., R.I. Radi and M. Ismail, 2006. Physiological responses of wheat (Triticum aestivum L.) germinating seeds to methyl tert-butyl ether (MTBE). Assuit Univ. J. Bot., 35: 121-128.

4:  Benkinney, M.T., J.F. Barbieri, J.S. Gross and P.A. Naro, 1994. Acute toxicity of methyl tert-butyl ether to aquatic organisms. 15th Annual SETAC Meeting 30 October-3 November, Denver, Co, USA.

5:  Bentsson, B.E. and M. Tarkpea, 1994. The acute aquatic toxicity of some substances carried by ships. Mar. Pollut. Bull., 14: 213-214.
CrossRef  |  

6:  Cape, J.N., I.D. Leith, J. Binnie, J. Content and M. Donkin et al., 2003. Effect of VOCs on herbaceous plants in an open-top chamber experiment. Environ. Pollut., 124: 341-353.
CrossRef  |  Direct Link  |  

7:  Fayolle, F.J., P. Vandecasteele and F. Monot, 2001. Microbial degradation and fate in the environment of methyl tert-butyl ether and related fuel oxygenates. Applied Microbiol. Biotechnol., 56: 339-349.
CrossRef  |  Direct Link  |  

8:  Franklin, P.M., C.P. Koshland, D. Lucas and R.F. Sawyer, 2000. Clearing the air: Using scientific information to regulate reformulated fuels. Environ. Sci. Technol., 34: 3857-3863.
CrossRef  |  Direct Link  |  

9:  Johansen, D.A., 1940. Plant Microtecnique. MC Book Company, New York, USA.

10:  Johnson, R., J. Pankow, D. Bender, C. Price and J. Zogorski, 2001. To what extent will past releases contaminate community water suppliers. Environ. Sci. Technol., 34: 210-214.

11:  Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
CrossRef  |  Direct Link  |  

12:  Lichtenthaler, H.K. and R.R. Wellburn, 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans., 11: 591-592.
CrossRef  |  Direct Link  |  

13:  Nellessen, J.E. and J.S. Fletcher, 1993. Assessment of published literature pertaining to the uptake/accumulation, translocation, adhesion and biotransformation of organic chemicals by vascular plants. Environ. Toxicol. Chem., 12: 2045-2052.
CrossRef  |  Direct Link  |  

14:  Rausina, G.A., C.L. Wong, W.R. Arnold, E.R. Mancini and A.E. Steen, 2002. Toxicity of methyl tert-butyl ether to marine organisms: Ambient water quality criteria calculation. Chemosphere, 47: 525-534.
CrossRef  |  Direct Link  |  

15:  Rousch, M. and M.R. Sommerfeld, 1998. Liquid-gas partitioning of the gasoline oxygenate Methyl Tert-Butyl Ether (MTBE) under Laboratory conditions and its effect on growth of selected algae. Arch. Environ. Contam. Toxicol., 34: 6-11.
CrossRef  |  Direct Link  |  

16:  Shehata, M.M., A. Habib, N.S. Khalifa and M.S. Salama, 2000. Cytological and biochemical effects of 5-florouracil and colchicine on Vicia faba plants. Egypt. J. Biotechnol., 7: 218-233.

17:  Squillace, P.J., J.S. Zogorski, W.G. Wilber and C.V. Price, 1996. Preliminary assessment of the occurrence and possible sources of MTBE in groundwater in the United States, 1993-1994. Environ. Sci. Technol., 30: 1721-1730.
Direct Link  |  

18:  Steel, R.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2nd Edn., McGraw Hill Book Co., New York, USA., ISBN-13: 9780070609266, Pages: 633.

19:  Yu, X.Z. and J.D. Gu, 2006. Uptake, metabolism and toxicity of methyl tert-butyl ether (MTBE) in weeping willow. J. Hazard. Mater., 137: 1417-1423.
CrossRef  |  Direct Link  |  

20:  Werner, I.C.S.C., L.A. Coger, Deanovic and D.E. Hinton, 2001. Toxicity of methyl tert-butyl ether to freshwater organisms. Environ. Pollut., 111: 83-88.
CrossRef  |  Direct Link  |  

©  2021 Science Alert. All Rights Reserved