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Research Article
 

Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia



Amor Gaddour, Sghaier Najari and Mabrouk Ouni
 
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ABSTRACT

A data issued from 16 years performances schedule of local goat, Alpine, Damascus, Murciana and crossed groups was used to study the genotypes productive behaviour under Tunisian oases conditions. The aim is to evaluate the possibilities of local goat productivity improvement by cross breeding in intensive mode and also, to choose the better improving breed and the propice crossing level. So, data of periodic individual weighing was used to estimates kid’s weight at some standard ages and dairy performances such as, daily milk average, total production by lactation and milking period of studied genetic goats groups. Statistical analysis of about 1928 kid’s weights and 1923 individual goat milking shows that, the cross breeding allows to improve the growth performances since the first generation with respect to local population production. ANOVA test shows an important effect of genotypes and environment upon kid’s weights (p<0.01). The kid’s weight average at birth and at 120 days age was about 3.49 and 15.78 kg, respectively. The cross breeding second generation allows the better improvement of local goat potentialities kid’s weight at 120 days reaches 16.19 kg for Damascus x local genotype. The dairy production with this generation is, about 248 kg for the Alpine one, of 181 kg for Damascus and 190 kg for Murciana, is only 137 kg by lactation for the local breed.

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Amor Gaddour, Sghaier Najari and Mabrouk Ouni, 2007. Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia. Pakistan Journal of Biological Sciences, 10: 2874-2879.

DOI: 10.3923/pjbs.2007.2874.2879

URL: https://scialert.net/abstract/?doi=pjbs.2007.2874.2879

INTRODUCTION

Genetic resources are the gene pool available for breeders and other scientists, and in the Triticeae tribe several pools are recognized (Von Botmer et al., 1992; Gill et al., 2006). Aegilops is characterized as a Mediterranean-Western Asiatic element and its center of diversity follows the central part of the Fertile Crescent arc in West Asia (Hegde et al., 2002).

Ae. cylindrica Host. (genome formula: CCDD) is an amphiploid, resulting from hybridization between the diploids Ae. caudata (CC) and Ae. squarrosa (DD). This species is, however, frequent in the Mediterranean area and in the Middle East, which is its center of distribution (Guadagnuolo et al., 2001). This species distributes of frequently along roadsides, edges of cultivation, dry hillsides, and grassy steppes, where it tolerates disturbance (van Slageren, 1994). It is known to hybridize spontaneously with wheat (Gandilyan and Jaaska, 1980; Snyder et al., 2000). Naturally formed hybrids between Ae. cylindrica and wheat occasionally produce seeds, presumably via spontaneous cross-pollination, which could facilitate gene flow between the D genomes of the two species (Gandilyan and Jaaska, 1980; Guadagnuolo et al., 2001; Galaev and Sivolap, 2005). Therefore, understanding the genetic structure of these troublesome weedy populations may facilitate designing new ways to control them, especially when most other conventional methods of weed control have failed to contain their spread (Donald and Ogg, 1991).

Today, molecular tools are available to evaluate and characterize, to a certain extent, genetic diversity among gene bank accessions. Genetic diversity of Ae. cylindrica has been evaluated using morphological data (Dotray and Young, 1993), isozyme analysis (Watanabe et al., 1994; Hegde et al., 2002), protein variation (Wan et al., 2002) and molecular markers (Okuno et al., 1998; Guadagnuolo et al., 2001; Pester et al., 2003). But although many landraces of wheats were collected in Iran and were available before and after the Islamic Revolution, wild wheat and Aegilops from Iran are still largely unknown. The objective of this study was to understand the extent and pattern of genetic diversity in 28 populations of allotetraploid wild wheat Ae. cylindrica, collected from roadside of Iran, using seed storage protein and RAPD markers.

MATERIALS AND METHODS

Plant material: Twenty eight populations of Aegilops cylindrica which collected from origin sites of Iran in 2004, including 10 provinces, (Table 1) were used in this study.

Storage protein analysis: In order to evaluate genetic diversity of high molecular weight subunit of glutenin within and between populations, three samples were taken and analysed from each of 28 populations of Ae. cylindrica. Chinies spring cultivar was used as a standard to compare subunit composition. The seed were crushed after removal of the embryo.


Table 1: Abbreviation, origin site and province of populations of Ae. cylindrica analyzed
Image for - Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia

The flour was mixed in an extraction buffer of 62 mM Tris-HCL (pH = 6.8) buffer containing 10% (W/V) glycerol, 4% (W/V) Sodium Dodecyl Sulfate (SDS), 0.01% (W/V) bromophenol blue and 4% ß-mercaptoethanol. Samples were allowed to stand at room temperature for at least 12 h with occasional vortexing. They were then placed in a boiling waterbath for 2 min and then centrifuged for 5 min at 6500 rpm and 15 μL of each sample was loaded on the gel. Proteins were fractionated by SDS-PAGE according modified of Laemmli (1970) procedure using stacking gel Proteins were fractionated using stacking gel containing 4% acrylamide, 0.05% bis acrylamide, 0.1% SDS and 0.00009 M Tris-HCL and separating gel containing 10% acrylamide, 0.13% bis acrylamide, 0.1% SDS and 6.78 M Tris-HCL. Gels were stained overnight with 0.01% (W/V) Coomassie Brilliant Blue R 250 in water and acetic acid (10%) and then destained overnight in water for at least 24 h.

RAPD assay: Total genomic DNA was isolated from young leaves of greenhouse-grown plants according to the CTAB protocol (Saghai-Maroof et al., 1984) with minor modifications. To reveal the level of genetic variation for each population, DNA of five plants were bulked and analysed. A total of fifteen 10-mer oligonucleotides from UBC (University of British Colombia) series were selected according to the number and consistency of amplified fragments. PCR reactions were carried out in a 25 μL-1 volume containing 1 unit of Taq polymerase, 5 ng of genomic DNA template, 0.2 μmol-1 of primer, 2 m μ of each dATP, dCTP, dGTP and dTTP, and 2.5 μL of 10X PCR reaction buffer. Amplifications were performed in a DNA Thermo-cycler (Perkin-Elmer) programmed for 45 consecutive cycles each consisting of 1 min at 92°C, 1 min at 37°C and 2 min at 72°C. Following amplification, the samples were subjected to electrophoresis in 6% acrylamide gels in TAE buffer running at 200 v for 2.5 h. The gels were stained using etidum bromide and viewed under Ultra-violet light.

Data analysis: Polymorphic RAPD fragments were scored as either present (1) or absent (0) across all populations. Only distinct, well-resolved fragments were scored. Binary matrix was used to estimate the genetic similarities between pairs, by employing Dice index (Nei and Li, 1979). These similarity coefficients were used to construct dendrogram using the Unweighted Pair Group Method with Arithmetic averages (UPGMA) employing the SAHN (Sequential, Agglomerative, Hierarchical, and Nested clustering) from the NTSYS-PC (Numerical Taxonomy and Multivariate Analysis System), version 2.02 (Applied Biostatistics) program (Rohlf, 1998). A principal coordinate analysis (PCO) was also conducted with the same program using the DCENTER and EIGEN procedures.

RESULTS

Protein analysis: The patterns of high molecular weight (HMW) glutenin seed proteins among 28 populations of jointed goatgrass detected that diversity in the three-band HMW glutenin pattern was extremely low. In other words, there was very low diversity within and between studied populations. In previous study, Wan et al. (2002) identified 3 subunits bands as 1Cx, 1Cy and 1Dy in all accessions of Ae. cylindrica. In this study, we also found 3 subunits bands for all within and between populations except in one sample. In this population from 3 samples analysed, two samples contained 3 subunits, but one of sample showed an extra band at top (1Dy) (Fig. 1).

RAPD analysis: In the RAPD analysis, 15 ten-mer primers were used to amplify all of the genotypes; 14 of these showed informative polymorphic products resolvable by gel electrophoresis. A total of 133 bands were screened (average of 8.9 bands per primer) among which 92 were polymorphic (69%). The number of fragments generated per primer varied between 3 and 16. The highest and the lowest number of polymorphic bands per assay unit were 0 (primer OPA-15) and 13 (primer UB-9), respectively (Table 2). Figure 2 shows an example of such a typical RAPD pattern using the WMS-43 primer.


Image for - Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia
Fig. 1: Example of High Molecular Weight (HMW) subunits of glutenin among some populations of Ae. cylindrica. Cs is chines spring; R1, R2 and R3 are three samples taken from each population

Image for - Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia
Fig. 2: Example of polymorphism between some populations of Ae. cylindrica as revealed by RAPD primer WMS-43

Table 2: RAPD primers with sequence, total number of bands, number of polymorphic bands, percent of polymorphism
Image for - Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia

Estimates of genetic similarity of RAPD based on 92 polymorphic markers between 28 populations of Ae. cylindrica ranged from 0.41 for AC17/AC24, AC13/AC17 and AC12/AC15 to 0.84 for AC20/AC23 with an average of 0.63.

Genetic similarity values were used for cluster analysis through UPGMA, resulting in a dendrogram (Fig. 3). Cluster analysis revealed that populations Ac7 and Ac17 from Mahabad and Shahrod origion sites, respectively were more genetically distinct from the others (Fig. 1) and the most obvious pairs were Ac20 and Ac23. PCO analyses revealed that for RAPD data the first two components of the PCO explained 14.8 and 9.1% of the total variation (Fig. 4).


Image for - Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia
Fig. 3: UPGMA dendrogram showing genetic relationships among the 28 populations of Ae. cylindrica used in this study. The dendrogram was constructed based on genetic similarity calculated according to Dice coefficient. For population abbreviations see Table 1

Image for - Kid’s Growth and Dairy Performances of Pure Breeds and Crossed Caprine Genotypes in the Coastal Oases of Southern Tunisia
Fig. 4: Patterns of relationships among 28 populations of Ae. cylindrica revealed by principal coordinate analysis based on RAPD data. For population abbreviations see Table 1

Although the results of PCO didn’t correspond totally to those from cluster analysis, but it confirmed some subgrouping obtained by cluster analysis.

DISCUSSION

In this study, two molecular methods, seed storage protein and RAPD, were used to investigate the genetic diversity in the 28 populations of Ae. cylindrica. Seed storage protein analysis showed very low genetic diversity in studied populations. Isozyme analysis also conducted by Watanabe et al. (1994) and Hegde et al. (2002) revealed little or no variation among accessions of jointed goatgrass. We could find an extra band at top (1Dy) that it was very similar to top band of Ae. triuncialis. As this species distributes frequently with Ae. triuncialis along roadsides, therefore it is possible that the accession with extra band is a hybrid between Ae. triuncialis and Ae. cylindrica.

Compare to seed storage protein, RAPD markers revealed more polymorphic fragments. This is expected, as proteins markers reflect only variation in the coding parts of the genome, which is by nature more conservative and thus less polymorphic. While RAPDs can detect variation in both coding and non coding sequences, and the length of the primers allows the amplification of a large number of fragments with a single primer (Guadagnuolo et al., 2001). The RAPD technique has been widely applied in studies of wheat genetic diversity (Cao et al., 1998; Sun et al., 2003; Naghavi et al., 2004; Ahmad Khan et al., 2005) and has shown its usefulness in rapid detection of genetic variation. Although the level of RAPD polymorphism within wheat itself has been reported to be low (Devos and Gale, 1992), but the level of RAPD polymorphism in wild wheat has been found to be higher than that in common bread wheat (Sun et al., 2003).

RAPD amplifications provided the largest set of polymorphic markers. In this study, 92 RAPD loci were used to investigate the genetic diversity in the populations of Ae. cylindrica. The amount of polymorphism found in this research (69%) was more than what reported in previous studies (Okuno et al., 1998, Guadagnuolo et al., 2001; Pester et al., 2003). These differences might be related to the utilization of different Ae. cylindrica germplasms as well as the used of different primers sequences.

In this research we found that there was little relationship between genetic divergence and geographical origins, so that the populations from similar geographical places (AC1 with AC11 or AC27) belonged to separate clusters. Conversely, populations from different geographical conditions (such as AC7 and AC17) relatively tended to be clustered in one part of the dendrogram.

In conclusion, our results show that compare to seed storage protein, RAPD is suitable for genetic diversity assessment in Ae. cylindrica populations and it appears to be the most suitable for this kind of study. This technique has the advantage of requiring very small quantities of template DNA and no prior knowledge of sequencing in the target genome (Pester et al., 2003). Even if the dominance of RAPD markers remains a problem for population genetic studies, it is possible to overcome it, if specific fragments for each taxon or group of taxa compared are produced (Guadagnuolo et al., 2001).

REFERENCES

1:  Alexandre, G., G. Aumont, J.C. Mainaud, J. Fleury and M. Naves, 1999. Productive performances of Guadeloupean Creole goats during the suckling period. Small Rumin. Res., 34: 155-160.
CrossRef  |  Direct Link  |  

2:  Alexandre, G., G. Aumont, J. Fleury, O. Coppry, P. Mulciba and A. Nepos, 1997. Semi-intensive production with the pasture the caprine ones with meat in wet tropical zone: The case of the Creole kids on pangola (Digitaria decumbens) in Guadeloupe. INRA Prod. Anim., 10: 43-53.

3:  Anastazios, N. and S.T. Ezzat, 2002. Goats husbandry for extensive use. Technical paper. Witzenhausen, pp: 14.

4:  Arbi, M., 2004. Effects of a food supplementation on the hormonal profile of the local goat in the tunisian south. Diploma of in-depth studies in dynamic biochemistry, Tunisian South, pp: 88.

5:  Awemu, E.M., B.Y. Nwakalor and Y. Abubakar, 1999. Environmental influences on pre-weaning mortality and reproductive performance of Red Sokoto does. Small Rumin. Res., 34: 161-165.
Direct Link  |  

6:  Caruolo, E.V., 1974. Milk yield, composition and somatic cells as a function of time of day in goats. Br. Vet. J., 130: 380-387.

7:  Chemineau, P., M. Mahieu, H. Varo, E. Shitalou and Y. Jego et al., 1991. Reproduction of caprine and the Creole sheep of Guadeloupe and Martinique. Rev. Elev. Md. Vt. Country Too. Special No., pp: 45-50.

8:  D' Aquino, P., P. Lhoste and A. Le Masson, 1995. Interactions Between the Systems of Production of Breeding and the Environment. Mixed Systems of Production Rain Agriculture and Breeding in Wetlands and Sub-Wet of Africa. Report/ratio CIRAD-EMVT, France, pp: 103

9:  Delgadillo, J.A., B. Malpaux and P. Chemineau, 1997. Reproduction of caprine in the tropical and subtropical zones. INRA Prod. Anim., 10: 33-41.

10:  DGPA., 2005. Statistics of the direction of the production and the Agricultural development. Ministry for Agriculture, pp: 45.

11:  Fabre-Nys, C., 2000. The sexual behavior of caprine hormonal and social factors. INRA Livestock Prod., 13: 11-23.

12:  Gaddour, A., 2005. Performances of growth and dairy production of the caprine genetic groups resulting from a crossing of absorption of the local goat in the oases of the Tunisian South. Mastre in Genetics and Bioressources, pp: 73.

13:  Gaddour, A., M. Ouni, M. Abdennebi, S. Najari and T. Khorchani, 2006. Improvement of the caprine performances in the oases by the crossing of absorption of the local goat. Rev. Arid. Reg., 18: 5-16.

14:  Goromela, E.H., I. Ledin and P. Uden, 1996. On-Farm Performance of Dual Purpose Goats and Farmers Attitudes Towards Introduction of Goats in HADO Areas of Kondoa. In: Integrated Farming in Human Development, Dolberg, F. and P.H. Petersen (Eds.). DSR-Forlag, Denmark
Direct Link  |  

15:  Hussain, S.S., P. Horst and A.B.M.M. Islam, 1995. Effect of different factors on pre-weaning survivability of Black Bengal kids. Small Rumin. Res., 18: 1-5.

16:  Jamali, M. and M. Villemeot, 1996. The Tunisian experiment as regards die caprine milk: The project of intensification of the dairy caprine breeding in the oases. Tunisian, PICO/OEP-UCARDEC. FAO., pp: 256.

17:  Morand-Fehr, P. and M. Doreau, 2001. Ingestion and digestion in the ruminants subjected to a stress of heat. Livestock Productions (INRA). Prod. Anim., 14: 15-27.

18:  Najari, S., 2005. Zootechnical and genetic characterization of a caprine population. Case of the local caprine population of the Tunisian arid areas, Ph.D Thesis, State, pp: 214

19:  Najari, S., A. Gaddour, M. Abdennebi, M.B. Hamouda and G. Khaldi, 2006. Morphological characterization of the local caprine population of the Tunisian arid areas. Rev. Arid Areas, 17: 23-41.

20:  Najari, S., A. Gaddour, M.B. Hamouda, M. Djemali and G. Khaldi, 2007. Growth model adjustment of local goat population under pastoral conditions in Tunisian arid zone. J. Agron., 6: 61-67.
CrossRef  |  Direct Link  |  

21:  Ouled, B.A., 2006. Ecological statute, biological performance and aptitude of the reinstalment of stipa lagascae r and sch in the degraded ecosystems of the Tunisian arid mediums. Ph.D. Thesis, Faculty of Science of Sfax.

22:  Ouni, M., 2006. Morphometric characterization of the goat genetic resources in the Tunisian arid zones. Master in Genetics and Bioressources, pp: 78.

23:  Pasquini, L.U., R.D. Ballou, R.D. Bremel and G.F. Greppi, 1994. Detection of protyletic degradation of milk protein and relationship with different levels of SCC in Italian goats. Production International Symposium Somatic Cell Counts and Milk of Small Ruminants, 25-27 September 1994, Bella. Italy.

24:  SPSS, 1998. SPSS Manual. Version 8.0, SPSS Inc., Chicago, IL

25:  Vernon, R.G., 1986. The Growth and Metabolism of Adipocytes. In: Control and Manupulation of Animal Growth, Buttery, P.J., N.B. Haynes and D.B. Lindsay (Eds.). Butter Worths, London, pp: 67-83

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