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Research Article

Genetic Variability and Population Structure in Beta-lactoglobulin, Calpastain and Calpain Loci in Iranian Kurdi Sheep

Mohammad Reza Nassiry, Feridoun Eftekhari Shahroudi, Mojtaba Tahmoorespur and Ali Javadmanesh
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The genotypes for Beta-Lactoglobulin (BLG) and calpastatin (CAST) were determined by Polymerase Chain Reaction (PCR) and restriction enzyme digestion and genotyped for calpain (CAPN) by PCR-SSCP method in an Iranian breed sheep, Kurdi. Blood samples were collected from 100 pure bred Kurdi sheep from Kurdi breeding station located in Shirvan, Mashhad. The extraction of genomic DNA was based on Guanidin Thiocyanate-Silica gel method. After PCR reaction, amplicons were digested with restriction enzymes MspI and RsaI for beta-lactoglobulin and calpastatin genes, respectively. The beta-lactoglobulin locus had 3 genotypes with frequencies of 0.24, 0.54 and 0.22 for AA, AB and BB, respectively; calpastatin genotypes had 2 genotypes with frequencies of 0.76 and 0.24 for MM and MN genotypes, respectively. Calpain genotypes were analyzed with SSCP method, which had 2 genotypes with frequencies of 0.92 and 0.08 for AA and AB, respectively. Heterozygosity value for beta-lactoglobulin locus was 49% and for calpastatin and calpain loci was very low (24 and 8%, respectively). χ2 test confirmed the Hardy-Weinberg equilibrium for three loci in this population. These data provide evidence that Iranian’s Kurdi sheep breed have a variability, which opens interesting prospects for future selection programs, especially marker-assistant selection between different genotypes of different locus and milk and cheese characteristics, gain and meat traits and also for preservation strategies.

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  How to cite this article:

Mohammad Reza Nassiry, Feridoun Eftekhari Shahroudi, Mojtaba Tahmoorespur and Ali Javadmanesh, 2007. Genetic Variability and Population Structure in Beta-lactoglobulin, Calpastain and Calpain Loci in Iranian Kurdi Sheep. Pakistan Journal of Biological Sciences, 10: 1062-1067.

DOI: 10.3923/pjbs.2007.1062.1067


1:  Anton, I., A. Zsolani and S. Kulkovics, 1997. Genetic polymorphisms of milk proteins in hungarian dairy sheep breeds and crosses. Proceedings of the International Workshop, (IW'97), Budapest, Hungary, pp: 224-226.

2:  Barillet, F., J.J. Arranz and A. Carta, 2005. Mapping quantitative trait loci for milk production and genetic polymorphisms of milk proteins in dairy sheep. Genet. Sel. Evol., 37: S109-S123.
CrossRef  |  Direct Link  |  

3:  Bastos, E., A. Cravador, J. Azevedo and H. Guedes, 2001. Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese Indigenous sheep breeds Churra da Terra Quente. Biochtechnol. Agron. Soc. Environ., 5: 7-15.
Direct Link  |  

4:  Bochkarev, V.V., 1998. The molecular analysis of beta-lactoglobulin locus in the different sheep breeds. Dubrovicy. Ph.D. Thesis. pp: 1-93.

5:  Boom, R., C.J. Sol, M.M. Salimans, C.L. Jansen, P.M.E. Wertheim-van Dillen and J. van der Noordaa, 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol., 28: 495-503.
PubMed  |  Direct Link  |  

6:  Caroli, A., P. Bolla and A. Spanu, 1995. Effect of beta-lactoglobulin genotype on milk yield in the Sardinian sheep. Procceding of the 11th National ASPA Congees, (ASPA'95), Udine, Italy, pp: 181-.

7:  Chung, H.Y., M.E. Davis and H.C. Hines and D.M. Wulf, 1999. Effect of the calpain proteolysis and calpain genotype on meat tenderness of Angus bulls. J. Anim. Sci., 77: 31-31.

8:  Chung, H.Y., M.S. Davis and H.C. Hines, 2001. Effect of calpain and calpastatin genotypes on growth of Angus bulls, research and reviews: Beef and sheep. Bull. Anim. Sci. Res. Rev., 156: 18-21.

9:  Eftekhari Shahroudi, F., M.R. Nassiry and R. Valizadeh, 2003. The genetic polymorphism of calpastatin gene in karakul sheep. J. Agric. Sci. Natl. Reso. Khazar, 2: 1-10.

10:  Elyasi, G., J. Shoja and M.R. Nassiry, 2004. Polymorphisms of β-lactoglobulin gene in Iranian sheep breed using PCR-RFLP. Proceedings of the Agriculture and Natural Resources Symposium, May 14-16, 2004, Ganja, Azerbaijan, pp: 66-72.

11:  Faraghi, A., A. Carta and F. Pilla, 1996. Beta-lactoglobulin polymorphism in sarda dairy sheep. Proccedings of the 47th Annul Meeting of the European Association for Animal Production, August 25-29, 1996, Lillehammer, pp: 42-.

12:  Garzon, A.I. and J. Martinez, 1992. β-Lactoglobulin in Manchega sheep breed: Relationship with milk technological index in handcraft manufacture of manchego cheesse. Anim. Genet., 23: 106-106.

13:  Gutierrez, G.B., J.J. Arranz and M.H. Othmane, 2001. Influencia del genotipo de la beta-lactoglobulina ovina sobre caracteres cualitativosy rendimiento quesero individual en la raza Churra. ITEA, 22: 15-17.

14:  Kocwin, M.P. and J. Kuryl, 2003. The effect of interaction between genotype at loci CAST, RYRI and RN on pig carcass quality and pork traits. Anim. Sci. Paper Rep., 21: 61-65.

15:  Kucinskiene, J., G. Vagonis, J. Maleviciute and I. Tapio, 2005. Genetic polymorphism of β-lactoglobulin in Lithuanian blackface and Lithuanian native coarse-woled sheep. Veterinarija ir zootechnika, 29: 90-92.
Direct Link  |  

16:  Kurly, J., W. Kapelanski and M. Pierzchala, 2002. Preliminary observations on the effect of calpastatin gene polymorphism in carcass trait in pigs. Anim. Sci. Paper Rep., 2: 87-95.

17:  Levene, H., 1949. On a matching problem in genetics. Ann. Math. Stat., 20: 91-94.
CrossRef  |  Direct Link  |  

18:  Lopez-Galvez, G., M. Ramos and P.J. Martin-Alvarez, 1993. Influence of milk protein polymorphism on cheese producing ability in the milk of Manchega ewes breed, cheese yield and factors affecting its control. Proceedings of the International Dairy Fed Seminar on Cheese Yield and Factors Affecting its Control, April 1993, Cork, Ireland, pp: 167-173.

19:  Nassiry, M.R., J. Shoja and G. Elyasi, 2002. The determination of beta-lactoglobulin gene polymorphism in some Iranian and Russian sheep breeds. Veterinarija ir zootechnika 19: 112-115.

20:  Palmer, B.R., J.G.H. Hickford and R. Bickerstaffe, 1997. A candidate gene approach to animal quality traits. Proc. N. Z. Soci. Anim. Prod., 57: 294-296.
Direct Link  |  

21:  Palmer, B.R., J.D. Morton and N. Roberts, 1999. Marker-assisted selection for meat quality and the ovine calpastatin gene. Proc. N. Z. Soc. Anim. Prod., 59: 266-268.
Direct Link  |  

22:  Tahmoorespour, M., 2005. Study on the calpain calpastatin gene polymorphisms by polymerase chain reaction-single strand conformation polymorphism and its relation to average daily gain in iranian baluchi sheep. Ph.D. Thesis. Ferdowsi University, Mashhad, Iran.

23:  Golijow, C.D., G. Giovambattista, M.V. Ripoli, F.N. Dulout and M.M. Lojo, 1999. Genetic variability and population structure in loci related to milk production traits in native Argentine Creole and commercial Argentine Holstein cattle. Genet. Mol. Biol., 22: 395-398.
CrossRef  |  Direct Link  |  

24:  Ali, S., M. McClenaghan, J.P. Simons and A.J. Clark, 1990. Characterisation of the alleles encoding ovine β-lactoglobulins A and B. Gene, 91: 201-207.
CrossRef  |  Direct Link  |  

25:  Nei, M. and W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA., 76: 5269-5273.
PubMed  |  Direct Link  |  

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