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Research Article
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Effects of Green Manures on Growth, Yield and Quality of Green Okra (Abelmoschus esculentus L.) Har Lium Cultivar |
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Chutichudet Benjawan,
P. Chutichudet
and
S. Kaewsit
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ABSTRACT
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Abstract: This green manure experiment with the use of okra crop as indicator plant was carried out at Mahasarakham University Experimental Farm, Mahasarakham province, Northeast Thailand during May to September 2005 to investigate four types of green manure legume crops on growth, yield and quality of edible fresh pods of okra crop when grown on Roi-Et soil series (Oxic Paleustults). The four types of green manure plants include Jack bean, Cowpea, Green gram, and Giant sensitive plant. The experiment consisted of five treatments, i.e., T1 (Control), T2 (Jack bean), T3 (Cowpea), T4 (Green gram) and T5 (Giant sensitive plant). The experiment was laid in a Completely Randomized Design (CRD) with four replications. The results showed that Roi-Et soil series (Oxic Paleustults) contained some considerable mean values of organic matter (1.64-1.66%) but soil available phosphorus and exchangeable potassium were relatively low, particularly potassium. Green manures of the four legume plants slightly improved soil property of the Roi-Et soil series (tested at the end of the experimental period). The most effective green manure on stem diameter, bushy diameter, leaf numbers plant-
1 and leaf area of the fifth leaf of the okra plants, in most cases, was found with Jack bean and Cowpea ranked the second. However, in most cases, Cowpea gave a similar effect as that of Green gram and Giant sensitive plant. Pod length and weight pod-
1, pod diameter and edible fresh pod yields (5,941.86 kg ha-
1) were highest with Jack bean green manure treatment (T2), whilst the rest, in most cases, were similar. Green manure treatments gave highly significant effect on total soluble solids of pods over the control treatment, whilst total acidity, fibre and pectin contents were unaffected by green manure treatments. Green manure of Jack bean was the best legume crop for green manure to be used in improving soil fertility, particularly for Roi-Et soil series (Oxic Paleustults).
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INTRODUCTION
Amongest many important kinds of vegetable crops, Green okra crop (Abelmoschus
esculentus L.) Har Lium Cultivar has its significant role in supplying nutritive
value to human daily diets. This crop could thrive on well in most tropical
and subtropical areas where fresh edible pods provide human supplementary vitamins
such as Vitamins C, A, B-Complex, iron, and calcium (FAO, 1988; Adebooye and
Opunta, 1996; Chutichudet et al., 2006). The pod mucilage has its medicinal
properties as an emollient, laxative and expectorant (Muresan and Popescu, 1993).
Edible fresh pods could be used for fat extraction for use in making brownies
(Boelje and Eidman, 1984). Furthermore, other forms of products derive from
okra include oven dried pods ready for use in soup preparations, candies and
salad dressing (Kalra and Pruthi, 1984; Owolarafe and Shotonde, 2004). With
these numerous uses of okra plants, thus many countries in Asia and Africa have
increased their land areas for the cultivation of this economic crop. They aim
to provide adequate amounts of edible fresh okra pods for domestic and overseas
consumption. In the 2004, Thailand exported okra fresh edible pods to Japan,
Germany, UK, Netherlands, Iran and other countries with an amount greater than
0.79 million US Dollars (Anonymous, 2004a). Growers of okra plants in Thailand
have annually increased a large land area for the cultivation of okra plants,
particularly those areas in the Central Plane and northeastern regions. Nevertheless,
growers in all regions of the country have come across with many problems such
as the use of insecticides and herbicides, which affected export quality standard
demanded by customers. Furthermore the problems on diseases and in particular
poor soil fertility are also having an important role in producing crop yields.
The latter has its significant impact in producing both high quality and yield
of the okra plants, thus the problem on poor soil fertility should receive priority
if both high edible pod yield and quality are to be expected. Recently, there
have been a number of workers who pointed out that organic agriculture has its
significant role on sustainable agriculture, since most tropical soils contained
small amounts of organic matter and soil deterioration could be most rapid due
to high leaching rate of soil nutrients caused mostly by rainfalls. Furthermore,
decomposition rate of soil organic matter is most rapid due to high environmental
temperatures (Rtnapradipa, 1996; Suksri, 1999; Liebman and Davis, 2000; Ngouajio
and McGiffen, 2002; Pholsen, 2003; Kasikranan, 2003). The problem on soil deterioration
is most severed in most soil series in Northeast Thailand, thus there is an
urgent need to improve soil fertility in the region in order to obtain high
crop yields (Anonymous, 2004b). One way to improve soil conditions is to add
green manure to the soil. Therefore, this investigation focused on the use of
four leguminous plant species as green manures added to the soil. They include
Sword bean or Jack bean (C. anavaria ensiformis L.), Cowpea (Vigna
unguiculata L.), Green gram (Vigna radiata L.) and Giant sensitive
plant (Mimosa invisa Mart). This work aims to describe the effects due
to each green manure on growth, yield and quality of okra crop when grown on
Roi-Et soil series (Oxic Paleustults). The obtained data may be useful to growers
of the okra plants.
MATERIALS AND METHODS
The experiment was carried out in the rainy season (May to September 2005) at Mahasarakham Experimental Farm, Mahasarakham University, Northeast Thailand to investigate effects of four types of green manure added to Roi-Et soil series (Oxic Paleustults) on growth, yields and quality of okra edible fresh pods (Abelmoschus esculentus L.) Har Lium Cultivar. The green manures used include Sword Bean or Jack Bean (C. anavaria ensiformis L.), Cowpea (Vigna unguiculata L.), Green Gram (Vigna radiata L.) and Giant sensitive plant (Mimosa invisa Mart). The initial mean values of soil pH, organic matter, nitrogen (%), available phosphorus (P) and exchangeable potassium (K) were 5.5, 1.64%, 0.05, 15.77 and 64.00 ppm, respectively. The layout of the experiment used was a Completely Randomized Design (CRD) with four replications. They consisted of 5 treatments, i.e., control (T1), Jack bean (T2), Cowpea (T3), Green gram (T4), and Giant sensitive plant (T5). The land area was ploughed twice followed by harrowing once. The plot size used was a 1.5x6 m with a path of 1.5 m in between the plots and all of the plots were added with an amount of a complete chemical fertilizer 15-15-15 (NPK) at a rate of 156.25 kg ha-
1. Seed weights of Jack bean, Cowpea, Green gram, and Giant sensitive plant used were 62.50, 50, 50 and 12.50 kg ha-
1. These amounts of seeds of each plant species were sown directly into their respective plots where appropriate (Anonymous, 2004b). Seeds of Jack bean and Giant sensitive plant were sown 10 days ahead of the rest in order to allow the four legume plant species to flower at the same time, i.e., both Jack bean and Giant sensitive plants were allowed to grow for 60 days, whilst both Cowpea and Green gram were allowed to grow for 50 days before they reached a stage of 50% flowering then they were incorporated into the soil with the use of a tractor unit (Anonymous, 2006). Ten days after the green manures were allowed to ferment in soil then a complete chemical fertiliser 15-15-15 (NPK) at a rate of 187.50 kg ha-
1 was evenly applied to all plots of all treatments used then 3-5 seeds hole of okra crop were sown by hand directly into their respective plots with distances between rows and within rows of 50x50 cm, respectively, i.e., a hectare has approximately 40,000 plants. Two weeks after emergence, seedlings were thinned out leaving only 2 seedlings hole-
1. To provide adequate amount of soil moisture content when there was no rainwater, furrow irrigation was given to a level of near field capacity (field capacity of the soil is approximately 13%) four times throughout the growth period. Weeding was carried out twice during the early growth period when okra leaf areas were relatively small then weeding was no longer needed due to a large amount of dense leaves covered the ground area. Benlate (Bennomyl), a fungicide chemical was sprayed where appropriate to the plants for 3 times before the okra plants reached their flowering stage. Lannate (Methomyl), an insecticide was also sprayed to the plants at two-week intervals starting from day 21 after emergence. The rates of both Benlate and Lannate used were as recommended by the chemical company (E. I. Dupont Thailand Co. Ltd.). The harvest for pod yields was carried out from time to time where appropriate starting from day 45 after emergence. The measurement parameters on growth include plant height (measured each stem from above ground level up to the upper most tip of leaves), stem diameter (measured at the point of the first true leaf), bushy diameter, number of leaves, edible fresh pod yields ha-
1 and leaf area of the fifth leaf (with the use of a portable leaf area meter, model AM-300, ADC Bioscience, England). These measurement parameters were carried out at 15 day intervals starting from day 15 after emergence until day 60, i.e., 4 measurement periods were carried out.
The measurement parameters on yield include pod length, pod diameter, fresh weight pod-
1, pod diameter, off standard pods and fresh pod yields ha-
1. Chemical analyses on quality of the okra edible pods include total soluble solids% (TSS), total acidity% (TA), fiber content% (FC) and pectin content% (PC). A hand refractometer E type, model N-1E, series 2111-W10, Japan was used for TSS, AOAC. method was used for TA (AOAC, 1984); Gould (1977) method was used for FC and Wattana-wanichakorn (1975) method was used for PC. The obtained data were statistically analysed where appropriate using a computer programme SPSS, Version 6 (SPSS, 1999).
RESULTS
Final soil analysis data: The results on soil analysis data taken from the final soil samples indicated that mean values of organic matter percentages found with soil samples treated with green manures increased slightly in all treated plots but remained the same with the control treatment with values ranged from 1.64 to 1.66% for T1 and T2, respectively (Table 1). A similar trend due to treatments was also found with pH, nitrogen, phosphorus, and potassium, i.e., in most cases, their values slightly increased, particularly soil exchangeable potassium with values ranged from 64 to 66 ppm for T1 and T2, respectively.
Plant height, stem and bushy diameters, leaf numbers and leaf areas: At day 15 after emergence, okra plant heights were similar in all treated plants with values ranged from 9.14 to 9.67 cm for T1 (control) and T5 (Giant sensitive plant), respectively (Table 2). For day 30 after emergence, the results showed that T2 (Jack bean green manure treatment) gave the highest followed by T3 (Cowpea), T4 (Green gram), T1 (control) and T5 (Giant sensitive plant) but all the treated plants, except T2, gave a similar value. The differences between T2 and the rest were statistically significant with values ranged from 18.17 to 21.54 for T5 and T2, respectively. At day 45, the results revealed that the highest plant height was attained with T2 followed by T3, T4, T5 and the lowest was found with T1 with values ranged from 32.15 to 41.80 cm for T1 and T2, respectively. The differences were large and highly significant. With the results at day 60, a similar trend due to treatments as that of day 45 was attained, i.e., T2 ranked the highest followed by T3, T4, T5 and T1 with values ranged from 47.24 to 61.20 cm for T1 and T2, respectively. The differences were large and highly significant.
With stem diameters, the results showed that at days 15 and 30, stem diameters of the okra plants were not affected by green manure treatments but at day 45 okra stem diameters were highly affected by green manure treatments, i.e., T2 ranked the highest followed by T3, T4, T5 and the lowest was found with T1 with values ranged from 2.16 to 2.72 cm for T1 and T2, respectively (Table 3). At day 60, the results showed that stem diameters of all green manure treated plants gave a similar significant value but highly significant over the control treatment (T1) with values ranged from 3.26 to 3.82 for T1 and T2, respectively. With bushy diameter, the results showed that there were no significant effects due to treatments at days 15 and 30 but significantly found at days 45 and 60. In most cases, bushy diameters of the green manure treated plants were similar but significantly greater than the control treatment (T1) with mean values at day 60 after emergence ranged from 53.20 to 67.58 cm for T1 and T2, respectively (Table 4). For leaf numbers, the results showed that leaf numbers at days 15 and 30 were not affected by green manure treatments but highly significant at days 45 and 60 after emergence (Table 5). Leaf number of T2 (Jack bean green manure) was highest, whilst other green manure treatments gave a similar leaf number with mean values at day 60 after emergence ranged from 23.80 to 31.98 leaves for T1 and T2, respectively. A similar trend to leaf numbers was found with leaf areas of the fifth leaf, i.e., there were no significant effects due to green manure treatments found at days 15 and 30 but significantly found at days 45 and 60 where T2 gave the highest but similar to T3 with mean values at day 60 ranged from 533.32 to 643.41 cm2 for T1 and T2, respectively (Table 6).
Pod length, pod weight and diameter, off standard pods and fresh pod yields:
The results showed that fresh pod length was longest with T2 (Jack bean) and
the shortest was found with T5 (Giant sensitive plant), which was significantly
lower than the control treatment with values ranged from 7.79 to 8.89 for T5
and T2, respectively (Table 7). The differences due to green manure treatments
were highly significant. For weight pod- 1, the results revealed
that all green manure treated plants were highly significant over the control
treatment where T2 was the highest whilst other green manure treated plants
were similar with values ranged from 9.18 to 12.10 g pod- 1 for T1
and T2, respectively. With pod diameter, the highest diameter was found with
T2 followed by T3 whilst other green manure treated plants gave a similar pod
diameter but T4 was similar to control treatment with values ranged from 2.09
to 2.38 cm for T4 and T2, respectively.
Table 1: |
Mean values of soil analysis data on organic matter % (OM),
pH values, total nitrogen, available phosphorus and exchangeable potassium
as influenced by leguminous green manure treatments |
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Table 2: |
Mean values of okra plant heights (cm) taken at days 15, 30,
45 and 60 after emergence as influenced by leguminous green manure treatments |
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Letter(s) within columns indicate least significant differences
(LSD) at p** = 0.01, * = 0.05, NS = Non Significant |
Table 3: |
Mean values of okra plant stem diameters (cm) taken at days
15, 30, 45 and 60 after emergence as influenced by leguminous green manure
treatments |
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Letter(s) within columns indicate least significant differences
(LSD) at *p = 0.01, NS = Non Significant |
Table 4: |
Mean values of okra plant bushy diameter (cm) taken at days
15, 30, 45 and 60 after emergence as influenced by leguminous green manure
treatments |
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Letter(s) within columns indicate least significant differences
(LSD) at *p = 0.05, NS = Non Significant |
Table 5: |
Mean values of leaf numbers/plant of okra plants taken at
days 15, 30, 45 and 60 after emergence as influenced by leguminous green
manure treatments |
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Letter(s) within columns indicate least significant differences
(LSD) at **p = 0.01, NS = Non Significant |
Table 6: |
Mean values of leaf area (cm2) of the fifth leaf
of okra plants taken at days 15, 30, 45 and 60 after emergence as influenced
by leguminous green manure treatments |
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Letter(s) within columns indicate least significant differences
(LSD) at *p = 0.05, NS = Non Significant |
Table 7: |
Mean values of pod length, pod weight, pod diameter, off standard
pods and fresh pod yields ha- 1 of okra plants as influenced
by leguminous green manure treatments |
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Letter(s) within columns indicate least significant differences
at **p = 0.01, *p = 0.05 |
Table 8: |
Mean values of total soluble solids, total acidity, fibre
content and pectin content of okra edible fresh pods as influenced by
leguminous green manure treatments |
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Letter(s) within columns indicate least significant differences
at **p = 0.01, NS = Non Significant |
For off standard pods, the results showed that percentages of off standard
pods were relatively high, i.e., T1 attained the highest followed by T5 where
T5 was similar to other green manure treated plants with values ranged from
21.49 to 38.37% for T2 and T1, respectively. With fresh pod yields, the results
revealed that T2 was the highest whilst other green manure treated plants gave
a similar pod yield to control treatment (T1) with values ranged from 3,502.95
to 5,941.86 kg ha- 1 for T1 and T2, respectively. The differences
were large and highly significant.
Total soluble solids, total acidity, fibre and pectin contents in pods: Chemical analysis showed that percentages of total soluble solids were highest with T2 but more or less similar to other green manure treated plants and the lowest was found with T1 with values ranged from 1.13 to 1.31% for T1 and T2, respectively. The differences were large and highly significant (Table 8). However, total acidity and fibre content due to treatments were not different from one another. Similarly, green manure treatments had no significant effect on pectin content of the okra plants with values ranged from 0.78 to 0.88% for T1 and T2, respectively.
DISCUSSION
The results on soil analysis data at the final sampling period indicated that
soil OM, pH, total nitrogen, available phosphorus, exchangeable potassium of
the plots treated with green manures, in most cases, have increased slightly.
The results suggested that green manures added to the soil had its significant
impact in improving soil conditions although some certain amounts of nutrients,
particularly nitrogen could have been taken up by the okra plants during the
growth period even though there had been an application of a complete chemical
fertilizer 15-15-15 (NPK) added to the soil before sowing but the added amount
failed to increase these macronutrients in soil. The results suggested that
an amount of 156.25 kg ha- 1 of the complete chemical fertilizer
added to the soil was not adequate enough to sustain a higher level of these
macronutrients. Thus the recommended rate being applied may not be suitable
for Roi-Et soil series (Oxic Paleustults) hence further work should be carried
out in order to justify the most suitable rate for this particular soil type.
Furthermore, It seems more likely that some further amounts of organic materials
are needed with this Roi-Et soil series apart from a complete chemical fertilizer
15-15-15 (NPK), since soil analysis data showed that only some small amounts
of soil nutrients have increased when green manures were added to the soil after
the crop was harvested. The decomposition of green manures added to the soil
improves soil conditions by increasing organic matter%, soil organic carbon
concentration, humus and polysaccharides have been reported (Biswas and Khosla,
1971; MacRae and Mehuys, 1985; Boparai et al., 1992; Suksri, 1999). Furthermore,
the improvement of soil conditions includes soil aggregation, pore spaces, bulk
density and ability to absorb a considerable amount of water (Mandal et al.,
2003). Nevertheless, it has been reported that in most areas in the tropics
soil deterioration could be most rapid due to a rapid decomposition rate as
a result of high soil temperatures (Miller and Donahue, 1992; Ratnapradipa,
1996; Suksri, 1999; Kasikranan, 2003; Pholsen, 2003). Thus growers of the crop
plants, particularly vegetable crops need to add more plant materials to their
soils annually. Rochester et al. (2001) reported that when leguminous
crops are grown and used for green manures they provide up to 40% of nitrogen
available in soils by the decomposition of nodules and other biomass of the
leguminous green manure crops.
With the present work, the effect due to green manure treatments on growth was relatively clear when the okra plants reached an age of 45 days after emergence, i.e., green manures of Jack bean, Cowpea, Green gram and Giant sensitive plant gave significantly higher plant height, stem diameter, bushy diameter, leaf numbers and leaf area of the fifth leaf than the control treatment. With respect to growth parameters, green manure of Jack bean, in most cases, attained the best results than the rest followed by Cowpea, whilst both Green gram and Giant sensitive plant gave similar growth parameters but significantly greater than the control treatment. The results indicated that green manure of Jack bean could be the best legume crop for use in improving soil fertility than the rest and Cowpea may be used as a second choice, whilst Green gram and Giant sensitive plant could be the last choice to be used in improving soil property. The differences found among the four legume crops may be attributable to the differences in the amounts of growth within a period till flowering. It could have been possible that Jack bean attained a greater biomass than the rest or it may be possible that this legume crop had produced a large amount of nodules than the rest. Gitari et al. (2003) showed that Jack bean was the most appropriate legume crop for use as a green manure material when okra crop is grown for high fresh edible pods. Kamidi et al. (2000) and Mukalama (2000) reported that Jack bean possesses a higher amount of growth of tops more than other legume crops hence its biomass could cover the ground area greater than other legume crops. MacRae and Mehuys (1985) stated that Jack bean could perform a deep anchorage roots than other legume crops being used thus it provided more soil pore spaces where more O2 could be adequately available for plant roots. Chaiyadok (1992) reported that living bacteria around roots and nodules of legume crops normally possess a selective property to its legume hosts. Thus an assay on the identification of different types of bacteria in roots of Jack bean may be useful in explaining the effectiveness of some specific microorganisms that enable to promote a rapid growth of the Jack bean plants.
The results on pod length and diameter, weight pod- 1 and pod yields
ha- 1 revealed that green manure of Jack bean (T2) gave the highest
and the differences were large and highly significant. Furthermore, percentages
of off standard pods were significantly lower than the control treatment but
similar to all green manure treated plants. The highest edible fresh pod yield
of T2 (5,941.86 kg ha- 1) signifies the utmost result of Jack bean
as a green manure legume crop. Thus growers of most cash crops should prepare
to multiply seeds of this legume crop annually. An analysis on total soluble
solids showed that Jack bean treatment (T2) gave the highest but similar to
both Giant sensitive plant and Green gram and these green manure treatments
were highly significant over the control treatment. The results indicated that
with this soil type green manure application is needed whenever okra crop is
to be grown for a better quality of edible fresh pods. With further work, it
may be of tangible value to analyze protein contents of the pods so that a full
detail of nutritive values could be described and grading standard may be specified.
However, with this work analyses on protein contents were not carried out. The
percentages on total acidity, fibre content and pectin of edible fresh pods
were not significantly different from one another. The results suggested that
green manure treatments had no significant effect on total acidity, fibre and
pectin contents. However, it was found that values on total acidity and fibre
content of the control treatment were higher than the manure treatments. This
may be attributable to the differences in the amounts of nitrogen in soil where
the green manure treatments could have contained more nitrogen than control
treatment, particularly during the growing period hence total acidity and fibre
content of the green manure treatments were slightly lower than the control
treatment. It was also found that mean values of available phosphorus and exchangeable
potassium were relatively low with mean values of 15.71 and 64 ppm, respectively.
These mean values of both soil available phosphorus and soil exchangeable potassium
may be considered to be inadequate for growth of most crops as stated by Miller
and Donahue (1990) and Suksri (1999) where the mean values should reach at least
20 ppm for available phosphorus and 80 ppm for exchangeable potassium. Although
an amount of a complete cheical fertilizer 15-15-15 (NPK) of 187.50 kg ha-
1 was added to the plots but their residual effects due to the complete
chemical fertilizer P and K added were not found. This may be partly attributable
to the utilization of the okra plants where a large amount of both P and K must
have been partly used apart from the loss of soil nutrients by leaching due
to both rainfalls and furrow irrigation. Thus the final values of both P and
K in soil were not relatively increased. Therefore, some further amounts of
chemical fertilizers P and K must be added to the soil if a further cultivation
of okra crop is to be carried out.
ACKNOWLEDGMENTS
The authors wish to express their sincere thanks to Mahasarakham University Financial Office for financial assistance. Professor Amnuaysilpa Suksri for his kind suggestion and assistance in preparing the manuscript. Thanks are also due to research staff members of the Faculty of Technology, Mahasarkham University for their assistance when this work was carried out.
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