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Research Article

Chronic Toxicity of Menazon and Relation to Oxidative Stress in Red Blood Cells of Rats

A.G. Ebadi and M. Shokrzadeh
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The effect of chronic exposure to menazon (Organophosphorus compound) in the production of oxidative stress was assessed in rats. Administration of menazon (50, 100, 500, 1000 ppm) for 2 weeks duration increased catalase (CAT), superoxide dismutase (SOD) activities in red blood cells (RBC). However, acetylcholinesterase (AChE) activity were decreased in these samples. The increase in RBC lipid peroxidation correlated well with the inhibition in RBC AChE activity. Increased activities of CAT and SOD showed significant correlations in RBC samples when different doses of menazon were used. The results of the present study suggest the usefulness of RBC AChE measurement as a good index in the evaluation of menazon-induced oxidative stress affecting blood.

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  How to cite this article:

A.G. Ebadi and M. Shokrzadeh , 2006. Chronic Toxicity of Menazon and Relation to Oxidative Stress in Red Blood Cells of Rats. Pakistan Journal of Biological Sciences, 9: 67-71.

DOI: 10.3923/pjbs.2006.67.71



1:  Prakasam, A., S. Sethupathy and S. Lalitha, 2001. Plasma and RBCs antioxidant status in occupational male pesticide sprayers. Clin. Chim. Acta, 310: 107-112.
CrossRef  |  PubMed  |  Direct Link  |  

2:  Storm, J.E., K.R. Karl and J. Doull, 2000. Occupational exposure limits for 30 organophosphate pesticides based on inhibition of red blood cell acetylcholinesterase. Toxicology, 150: 1-29.
Direct Link  |  

3:  Kale, M., N. Rathore, S. John and D. Bhatnagar, 1999. Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: A possible involvement of reactive oxygen species. Toxicol. Lett., 105: 197-205.
CrossRef  |  Direct Link  |  

4:  Lawrence, T., L.T. McGrath, A.F. Douglas, E. McClean, J.H. Brown and C.C. Doherty, 1995. Oxidative stress and erythrocyte membrane fluidity in patients undergoing regular dialysis. Clin. Chim. Acta, 235: 179-188.
CrossRef  |  

5:  Halliwell, B., 1994. Free radicals, antioxidants and human disease: Curiosity, cause, or consequence? Lancet, 344: 721-724.
CrossRef  |  PubMed  |  Direct Link  |  

6:  Banerjee, B.D., V. Seth, A. Bhattacharya, S.T. Pasha and A.K. Chakraborty, 1999. Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers. Toxicol. Lett., 107: 33-47.
CrossRef  |  Direct Link  |  

7:  Abdollahi, M., A. Jafari and N. Jalali, 1995. Chronic toxicity in organophosphate exposed workers. Med. J. Islamic Republic Iran, 9: 221-225.

8:  Ranjbar, A., P. Pasalar and M. Abdollahi, 2002. Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorous pesticide manufacturing workers. Hum. Exp. Toxicol., 21: 179-182.
CrossRef  |  Direct Link  |  

9:  Aebi, H., 1984. Catalase in vitro. Meth. Enzymol., 105: 121-126.
CrossRef  |  PubMed  |  Direct Link  |  

10:  Ukeda, H., S. Maeda, T. Ishii and M. Sawamura, 1997. Spectro-photometric assay for superoxide dismutase based on tetrazolium salt 3'{l-[(phenylamino)-carbonyl]-3,4-tet-razolium}-bis(4-methoxy-6-nitro) benzensulfonic acid hydrate reduction by xanthine-xanthine oxidase. Anal. Biochem., 251: 206-209.
CrossRef  |  

11:  George, P.M. and M.H. Abernethy, 1983. Improved ellman procedure for RBC cholinesterase. Clin Chem., 29: 365-368.

12:  Koner, B.C., B.D. Banerjee and A. Ray, 1998. Organochlorine pesticide-induced oxidative stress and immune suppression in rats. Ind. J. Exp. Biol., 36: 395-398.
PubMed  |  

13:  Samsheshekariaiah, B.V., K. Padmaja and A.R.K. Prasad, 1992. Lead induced lipid peroxidation and antioxidant defense components of developing chick embryos. Free Radio Biol. Med., 13: 107-114.
Direct Link  |  

14:  Geller, B.L. and D.R. Winge, 1984. Sub cellular distribution of superoxide dismutase in rat liver. Methods Enzymol., 105: 105-114.
PubMed  |  

15:  Yang, Z.P., J. Morrow, L.J. Roberts and W.D. Dettbarn, 1996. Diisopropylphosphorofluoridate-induced muscle hy-peractivity associated with enhanced lipid peroxidation in vivo. Biochem. Pharmacol., 52: 357-361.
PubMed  |  

16:  Datta, C., J. Gupta and D. Sengupta, 1994. Interaction of organophosphorus insecticides phosphamidon and malathion on lipid profile and acetylcholinesterase activity in human RBC membrane. Indian J. Med. Res., 100: 87-89.
PubMed  |  

17:  Ahmed, R.S., V. Seth, S.T. Pasha and B.D. Banerjee, 2000. Influence of dietary ginger (Zingiber officinales Rosc) on oxidative stress induced by malathion in rats. Food Chem. Toxicol., 38: 443-450.
CrossRef  |  Direct Link  |  

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