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Haematological Parameters in Rabbit Breeds and Crosses in Humid Tropics



C.A. Chineke, A.G. Ologun and C.O.N. Ikeobi
 
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ABSTRACT

Blood samples collected on 206 rabbits from breeding experiment conducted at rabbit unit of Teaching and Research, Federal University of Technology, Akure were analysed to study the effects of genotype, sex and age on some haematological values namely Packed Cell Volume (PCV), hemoglobin concentration (HBC), Red Blood Cells (RBC), White Blood Cells (WBC), Mean Corpuscular Haemolobin (MCH), Mean Corpuscular Haemolobin Concentration (MCHC), Mean Corpuscular Volume (MCV) and Erythrocyte Sedimentation Rate (ESR). The 206 rabbits, 59 males and 147 females used in the study comprised 7 genetic groups viz 54 New Zealand whitexNew Zealand white (NZWxNZW), 65 ChinchillaxChinchilla (CHAxCHA), 17 ChinchillaxNew Zealand white Dutch belted (CHAxNZWDBD), 28 New Zealand white Dutch beltedxNew Zealand white Dutch belted (NZWDBDxNZWDBD), 14 New Zealand whitexNew Zealand white Dutch belted (NZWxNZWDBD),7 New Zealand white CroelxNew Zealand white Croel (NZWCRLxNZWCRL) and 11 New Zealand whitexChinchilla (NZWxCHA), were aged 3-4, 5-6, 7-8, 9-10 and 11-12 months and above. The least squares model included main effects of genotype, sex and age as factors among others that contribute to variations in haematological values in rabbits. There was genotype differences (p<0.05) in PCV, WBC, MCH and ESR. Genotype NZWxCHA recorded highest mean values in PCV, HBC and RBC. The haematological variables by sex were not different (p>0 .05) except in ESR where females recorded higher significant (p<0.05) mean value than the males. Age effect were obtained (p<0.001) for WBC and ESR and for ((p<0.05) HBC, MCH and MCHC. The haematological values reported in this study could be useful for reference in health management of rabbits of different genotypes, sexes and ages in the humid tropics.

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  How to cite this article:

C.A. Chineke, A.G. Ologun and C.O.N. Ikeobi , 2006. Haematological Parameters in Rabbit Breeds and Crosses in Humid Tropics. Pakistan Journal of Biological Sciences, 9: 2102-2106.

DOI: 10.3923/pjbs.2006.2102.2106

URL: https://scialert.net/abstract/?doi=pjbs.2006.2102.2106

INTRODUCTION

The life of all flesh is the blood and its usefulness for atonement for human soul (James, 2004), for assessing the health status, clinical evaluation for survey, physiological/pathological conditions and diagnostic and prognostic evaluation of various types of diseases in animals (Taiwo and Anosa, 1995; Awah and Nottidge, 1997, 1998; Nottidge et al., 1999; Kral and Suchy, 2000; Padilla et al., 2000; Tambuwal et al., 2002; Singh et al ., 2002; Obasoyo et al., 2005; Alade et al., 2005; Amel et al., 2006) have been reported. The genetic and non-genetic factors affecting biochemical and haematological parameters of animals have also been observed (Kleinbeck and McGlone, 1999; Agaie and Uko, 1998; Svoboda et al., 2005).

The examination of blood provides the opportunity to clinically investigate the presence of several metabolites and other constituents in the body of animals and it plays a vital role in the physiological, nutritional and pathological status of an organism (Aderemi, 2004; Doyle, 2006). It also helps in distinguishing normal state from state of stress, which can be nutritional, environmental or physical (Aderemi, 2004). Schalm et al. (1975) reported that blood pictures of animal might be influenced by certain factors such as nutrition, management, breeds of animals, sex, age, diseases and stress factors. Besides, physiological and environmental factors that might affect blood values as: age of the animal such as estrus cycle, pregnancy and parturition, genetics, method of breeding, breeds of animal, housing, feeding, fasting, extreme climatic conditions, stress, exercise, transport, castration and diseases had been identified (Carlson, 1996; Johnston and Morris, 1996).

The commonly used haematological parameters are erythrocytes (Red Blood Cells, RBC), leucocytes (White Blood Cells, WBC), hemoglobin concentration (HBC), Packed Cell Volume (PCV) and values which include Mean Corpuscular Volume or cell (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC) (Carlson, 1996). Specific causes of erythrocytic abnormalities, which might manifest in chronic blood loss, include bloody diarrhoea, ulcers, bleeding, neoplasm and blood sucking parasites (Johnston and Morris, 1996).

A lot of work has been carried out on the blood parameters of various domestic animals and livestock (Solomon et al., 1998; Kral and Suchy, 2000; Singh et al., 2002; Svoboda et al., 2005; Ahamefule et al., 2006). But there is a dearth of information on the reference haematological values of rabbits in the humid tropics, confirming the fact that the rabbit has received little or no attention in the developing nations. This study was therefore designed to determine the effects of some factors such genotype, sex and age on haematological parameters of rabbit breeds and crosses in the humid tropics of Nigeria.

MATERIALS AND METHODS

Location of study: The study was conducted (between 1998 and 2001) in the rabbit unit of the Teaching and Research Farm of the Federal University of Technology, Akure, Nigeria. Akure is situated on 350.52 m above sea level at latitude 7o14’N and at longitude 5o14’E. The city falls within the rainforest zone of the humid tropics which is characterized by hot and humid climate. The mean annual rainfall is 1500 mm and the rains period is bimodal with a short break in August. The mean annual relative humidity is 75% and that of temperature is 20°C.

Animal and their management: A total of 206 rabbits obtained from a cross breeding experiment conducted at rabbit unit of Teaching and Research Farm, Federal University of Technology, Akure were used in the study. The rabbits, 59 males and 147 females were distributed into 7 genotypes as shown: 54 New Zealand whitexNew Zealand white (NZWxNZW), 65 ChinchillaxChinchilla (CHAxCHA), 17 ChinchillaxNew Zealand white Dutch belted (CHAxNZWDBD), 28 New Zealand white Dutch beltedxNew Zealand white Dutch belted (NZWDBDxNZWDBD), 14 New Zealand whitexNew Zealand white Dutch belted (NZWxNZWDBD),7 New Zealand white CroelxNew Zealand white Croel (NZWCRLxNZWCRL) and 11 New Zealand whitexChinchilla (NZWxCHA). The age range of the rabbits were recorded as 3-4, 5-6, 7-8, 9-10 and 11-12 months and above. The genotypes evolved from mating between 49 does (15 New Zealand white (NZW) and 13 Chinchilla (CHA) purebreds, 11 New Zealand whitex Dutch belted (NZWDBD), 10 New Zealand whitexCroel (NZWCRL) crossbreds) and 13 bucks (3 NZW and 5 CHA purebreds and 3 NZWDBD and 2 NZWCRL crossbreds ). The does were randomly assigned to the bucks for mating early in the morning. The kits were sexed at 21 days. Litters were weaned at 35 days when each kit was individually ear-tagged and weighed. Litter mates were kept together in the same cage to 56 days of age. Thereafter, the rabbits were separated into individual cages provided with feeding and watering troughs, which were made from tins.

The rabbits were given ad libitum access to commercial diet of 15% crude protein and 2300 kcal kg-1 metabolizable energy in the morning, supplemented with sweet potato leaves and Aspillia africana in the evening.

The incidence of diarrhea was combated with antibiotics such as embassin forteR. To ensure absence of haemoparasites, internal and external parasites, the animals were treated with IVOMECR injection.

Collection of blood: About 5 mL blood withdrawn from ear vein of each animal by means of 5 mL sterile needle and syringe was expelled gradually into labeled bijou bottle containing a speck of dried ethyldiamine tetracetic acid (EDTA) powder. The bottles were immediately capped and the contents mixed gently for about a minute by repeated inversion. Blood collected this way was used for the various haematological studies.

Haematological studies: The haematological parameters were determined following the methods outlined in Lamb (1981). This is described here briefly. The Packed Cell Volume (PCV) was determined by spinning about 75 UL of each blood sample in heparinized capillary tubes in a hematocrit micro centrifuge for 5 min. The Red Blood Cell (RBC) count and White Blood Cell (WBC) count were estimated using normal saline as the diluting fluid. The hemoglobin concentration (HBC) was estimated using cyanomethemoglobin method while the Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC) and the Mean Corpuscular Volume (MCV) were calculated by Lamb (1981) procedure.

Analytical procedures: The effects of genotype, sex and age on the blood parameters were estimated from least-squares procedures of unequal subclass numbers according to the method of Harvey (1999). Where significant differences were observed, differences among means were tested using the Duncan’s multiple range procedures outlined in the Harvey (1999).

RESULTS

Genotype was an important source of variation (p<0.05) for PCV, WBC, MCH and ESR. Age strongly influenced (p<0.001) WBC and ESR. Also age effect (p<0.05) was observed for HBC, MCH and MCHC.

Table 1: Mean Squares (MS) for haematological indices
Image for - Haematological Parameters in Rabbit Breeds and Crosses in Humid Tropics
*p<0.05,**p<0.01,***, p<0.001

Table: 2: Least square means for haematological indices
Image for - Haematological Parameters in Rabbit Breeds and Crosses in Humid Tropics
Means with different superscripts in the same column are significantly different (p<0.05, p<0.01, p<0.001)

The effect of sex was similar for all the variables except for ESR (p<0.05). The interaction between genotype x sex was not an important of variation (Table 1).

Genotypes differed in mean values for PCV, WBC, MCH and ESR but were similar in HB, RBC, MCHC and MCV. The highest PCV%, HBC and RBC values were recorded for NZWxCHA while the lowest values of the same parameters were observed for CHAxNZWDBD (27.82±1.94%), NZWxNZWDBD (8.47±0.42 g/100 mL and CHAxNZWDBD (3.24±0.29106 μm-3). For MCH, MCHC, MCV and ESR, the highest means were observed for CHAxNZWDBD while the lowest values for the same parameters were obtained for NZWxCHA (28.47±1.80), NZWxNZWDBD (81.87±5.19) and NZWxCHA (1.18±0.69) (Table 2).

The means of the indices by sex were not statistically (p>0.05) different. However, the males were higher in PCV%, HBC, RBC, MCH and MCHC than the females. Females were only higher than males in WBC, MCV and ESR.

The age differed significantly (p<0.05) in mean values for HBC, WBC, MCH, MCHC and ESR but was similar in PCV, RBC and MCV. The highest PCV% (30.97±1.18) was recorded at 11-12 above, HBC (9.16±0.25 g/100 mL) at 3-4 months, RBC (3.63±0.14 106 μm-3) at 7-8 months; MCH (27.24±1.34), MCHC (31.75±1.12) at 3-4 months; WBC (8.05±0.25 106 m-3) at 7-8 months; MCV (91.96±3.55) at 11-12 above and ESR (3.16±0.26) at 9-10 months ( Table 2 ).

DISCUSSION

The range of haematological indices in the rabbits understudy agreed closely as reported elsewhere (Kral and Suchy, 2000; Padilla et al., 2000; Tambuwal et al., 2002; Singh et al., 2002; Schalm et al., 1975; Johnston and Morris, 1996; Solomon et al., 1998). Genotype influence (p<0.05) on PCV, WBC, MCH and ESR was observed. Schalm et al. (1975) reported significant breed differences between haematological values of New Zealand White and wild jackrabbits. Generally, haematological studies of various farm animals showed either no significant or significant breed effects (Schalm et al., 1975). The values obtained for PCV were higher than that reported by Solomon et al. (1998). Kopp and Hetesa, (2000) reported that high PCV haematocrit reading indicated either an increase in the number of circulating RBC or reduction in circulating plasma volume. Adejumo (2004) reported that haemotological traits especial PCV and HB were correlated with the nutritional status of the animal. However, the primary functions of the erythrocyte are to serve as a carrier of hemoglobin. It is thus hemoglobin that reacts with oxygen carried in the blood to form oxyhemoglobin during respiration.

The HBC, RBC, MCHC and MCV values were identical in all the genotypes. This might point to the fact that all the blood samples collected and analysed had similar cellular hemoglobin content. However, MCHC is very significant in the diagnosis of anaemia and also serves as a useful index of the capacity of bone marrow to produce red blood cells (Awodi et al., 2005).

The non-significant sex effects on the haematological variables were obvious in this study except ESR. This agreed with the study of Schalm et al. (1975) which found no sex effect on rabbit and sheep haematology, but contrasted with the sex significant effect reported in dogs (Awah and Nottidge, 1997). Several haematological reports involving farm animals showed no sex effect (Nottidge et al., 1999; Singh et al., 2002).

Age had significant influences on HBC, WBC, MCHC and ESR. Similar age effects had been reported in various animal species (Schalm et al., 1975). In the present study, the haematological variables with age did not follow any particular trend in their variation. In horses, the MCV, MCH and MCHC consistently increased with age but WBC was highest in the youngest group (Schalm et al., 1975).

The ESR with regard to the blood physical properties was low. ESR was believed to be mostly determined by the frictional resistance of the surrounding plasma, which holds the cells in suspensions and the gravitational pull on the erythrocyte. The values obtained in the present study should not be a source of worry since they were very low. Similar sedimentation rates had been reported elsewhere (Fasuyi et al., 2005).

Apart from these factors considered in the study, differences in haematological indices may be caused by nutritional, environmental and hormonal factors. However the results obtained in this study compared closely with reports in available literature. There is still the need to have more reference values of haematological parameters of domestic rabbit in the humid tropics.

REFERENCES

1:  Adejumo, D.O., 2004. Performance, organ development and haematological of rats fed sole diets of graded levels of cassava flour and soybean flour (Soygari) as substitutes for energy and protein concentrates. Trop. J. Anim. Sci., 7: 57-63.

2:  Aderemi, F.A., 2004. Effects of replacement of wheat bran with cassava root sieviate supplemented or unsupplemented with enzyme on the haematology and serum biolochemistry of pullet chicks. Trop. J. Anim. Sci., 7: 147-153.

3:  Agaie, B.M. and O.J. Uko, 1998. Effects of season, sex and species difference on Packed Cell Volumes (PCV) of Guinea and domestic fowl in Sokoto, Nigeria. Vet. J., 19: 95-99.

4:  Ahamefule, F.O., G.O. Eduok, A. Usman, K.U. Ahamefule, Obua, B.E. and S.A. Ogunike, 2006. Blood chemistry and haematology of weaner rabbits fed sun dried, ensiled and fermented cassava peel based diets. Pak. J. Nutr., 5: 248-253.

5:  Alade, A.A., A.M. Bambose, E.B. Oguntona and A.O. Fanimo et al., 2005. Haematological parameters, serum metabolitescarcass characteristics of weaner rabbits fed yam peel meal diets. Proceedings of 10th Annual Conference of Animal Science Association of Nigeria Dairo, Sept. 12-15, University of Ado-Ekiti Nigeria, pp: 280-282

6:  Amel, O.B., S.A. Mariam, A.S. Ehsan and M.A.S. El-Badwi, 2006. Some biochemical values in the young and adult Sudanese geese Anser anser. J. Anim. Vet. Adv., 5: 24-26.
CrossRef  |  Direct Link  |  

7:  Awah, J.N. and H.O. Nottidge, 1997. Haematological and serum biochemical parameters in clinically healthy dogs in Ibadan, Nigeria. Trop. Vet., 16: 3-4.

8:  Awah, J.N. and H.O. Nottidge, 1998. Serum biochemical parameters in clinically healthy dogs in Ibadan. Trop. Vet., 16: 123-129.

9:  Awodi, S., J.O. Ayo, A.D. Atodo and T. Dzende, 2005. Some haematological parameters and the erythrocyte osmotic fragility in the laughing dove (Streptopella senegalensis) and the village weaver bird (Ploceus cucullatus). Proceedings of 10th Annual Conference of Animal Science Association of Nigeria, September 12-15, 2005, University of Ado-Ekiti, Nigeria, pp: 384-387

10:  Carlson, G.P., 1996. Clinical Chemistry Tests. In: Internal Animal Medicine, Smith, B.P. (Ed.). 2nd Edn., Mosby Publishers, USA

11:  Doyle, D., 2006. William Hewson (1739-74): The father of haematology. Br. J. Haematol., 133: 375-381.
Direct Link  |  

12:  Fasuyi, A.O., K.S. Fajemilehin and S.O. Aro, 2005. Nutritional potential of Siam weed (Chromolaena odorata) leaf meal (SWLM) on laying hens: Biochemical and haematological implication. Pak. J. Nutr., 4: 336-341.

13:  Harvey, W.R., 1999. User`s Guide for LSMLMW: Mixed Model Least Squares and Maximum Likelihood Computer. The Ohio State University, Colombus, Mimeo

14:  James, K.V., 2004. The Holy Bible Authorised King James Version. Vol. 17, Minsk, Belarus for Jet Move International Limited, Dallas, USA., pp: 11-72

15:  Johnston, J.K. and D.D. Morris, 1996. Alterations in Blood Proteins. In: Internal Animal Medicine, Smith, B.P. (Ed.). 2nd Edn., Mosby Publishers, USA

16:  Kleinbeck, S. and J. McGlone, 1999. Intensive indoor vs outdoor production system: Genotype and supplemental iron effects on blood haemolobin and Selected immune measures in zouny pigs. J. Anim. Sci., 77: 2384-2390.

17:  Kopp, R. and J. Hetesa, 2000. Changes of haematological indices of juvenile carp (Cyprinus carpio L.) under the influence of natural populations of cyanobacterial water blooms. Acta Vet. Brno., 69: 131-137.
Direct Link  |  

18:  Kral, I. and P. Suchy, 2000. Haematological studies in adolescent breeding cocks. Acta Veterinaria Brno, 69: 189-194.
CrossRef  |  Direct Link  |  

19:  Lamb, G.M., 1981. Manual of Veterinary Laboratory Techniques in Kenya. Ministry of Livestock Development/CIBA GEIGY, Basle, Switzerland, pp: 96-107

20:  Nottidge, H.O., V.O. Taiwo and A.O. Ogunsanmi, 1999. Haematological and serum biochemical studies of cats in Nigeria. Trop. Vet. Sci., 17: 9-16.

21:  Obasoyo, D.O., A.M. Bamgbose and S.O. Omoikhoje, 2005. Blood profile of broilers fed diets containing different animal protein feedstuffs. Proceedings of 10th Annual Conference of Animal Science Association of Nigeria. Sept. 12-15, University of Ado-Ekiti, Nigeria, pp: 155-157

22:  Padilla, S., J. Bouda, G.F. Quiroz-Rocha, J.L. Davalos and A. Sanchez, 2000. Biochemical and haematological values in venous blood of farmed Red Deer (Cervus elaphus) at high altitude. Acta Vet. Brno., 69: 327-331.

23:  Schalm, O.W., N.C. Jain and E.J. Caroll, 1975. Veternity Haematology. 3rd Edn., Lea and Fabiger, Philadelphia, pp: 15-218

24:  Singh, A.S., D.T. Pal, B.C. Mandal, P. Singh and N.N. Pathak, 2002. Studies on changes in some of blood constituents of adult cross-bred cattle fed different levels of extracted rice bran. Pak. J. Nutr., 1: 95-98.
CrossRef  |  Direct Link  |  

25:  Solomon, I.P., A. Monsi and F.L. Zitte, 1998. Physiological, haematological and biochemical evaluation of grasscutter and rabbit. Proceedings of Silver Anniversary Conference of Nigerian Society for Animal Production, Mar. 21-26, Abeokuta, Nigeria, pp: 405-406

26:  Svoboda, M., K. Eichlerova, V. Horak and Hradecky, 2005. Development of haematological indices in melanoma-bearing Libechov minipigs. Acta Vet. Brno., 74: 603-611.

27:  Taiwo, V.O. and V.O. Anosa, 1995. Fibrinogen, leukocyte and haematocrit values of cattle with various disease conditions. Trop. Vet., 13: 51-57.

28:  Tambuwal, F.M., B.M. Agaie and B. Bangana, 2002. Aematological and biochemical values of apparently healthy red sokoto goats. Proceedings of 27th Annual Conference of Nigerian Society for Animal Production, March 17-21, 2002, Akure, Nigeria, pp: 50-53

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