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Research Article
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Effect of Salinity on in vitro Trichoderma harzianum Antagonism Against
Verticillium dahliae |
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Amina Regragui
and
Houria Lahlou
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ABSTRACT
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This in vitro study led to test the sodium chloride tolerance
of Trichoderma harzianum and to evaluate the impact of salinity on its
antagonistic capacities, in order to use it as a biological agent controlling
Verticillium wilt of tomato in Moroccan saline soils. Trichoderma
harzianum tolerates the salinity for its mycelial growth but its sporulation
is significantly reduced. The efficiency of the antagonistic modes of action
of Trichoderma against Verticillium depends on the saline concentration
used. Thus, the antagonism by competition is slightly affected by moderated
salt concentrations (6 g L-1). The antagonism by antibiosis decreases
with the increase of salt. The antagonistic compounds produced by Trichoderma
in presence of 8 g L-1 NaCl concentration still reduce the pathogen
growth but have no action on the abundance of microsclerotia. The in vitro
antagonistic properties of T. harzianum obtained at low salt concentrations
may be applied for the biological control of Verticillium wilt of tomato
in Moroccan saline soils where levels of salinity are equivalent to salt concentrations
favorable to Trichoderma antagonism.
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