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Research Article

Pre-biotic Effects of Sobo Drink on Colonization Resistance to Experimental Infection with Staphylococcus aureus 8588 in Rats

Anthony Olufemi Ajayi , Olumuyiwa Sunday Falade and Steve Adeniyi Adewusi
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This study investigated the effects of the continuous consumption of Sobo on colonization resistance to experimental infection in rats. Four matched groups of male and female adult Wister rats were orally administered H. sabdariffa calyx infusion at 37.6 and 100.0 mg mL-1 concentrations while the four control groups received distilled water once daily for 15 days. All rats were challenged on day 16 with 3.0-3.5X105 viable S. aureus 8588 CFU in 0.5 mL phosphate buffer and sacrificed on day 21. Faecal samples were collected six times (days 0-21) for dry mass index, coliform and S. aureus load. Blood was analysed for S. aureus antibody and lymphocyte count. Data were subject to statistical analyses. Faecal coliform counts were significantly depressed in Sobo administered groups with increased S. aureus shedding. Lymphocyte counts were however not significantly different and S. aureus antibody was not detected in the serum of rats. Sobo seems to possess pre-biotic properties and its continuous consumption may contribute to the elevation of colonization resistance to infection in the gastrointestinal tract.

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  How to cite this article:

Anthony Olufemi Ajayi , Olumuyiwa Sunday Falade and Steve Adeniyi Adewusi , 2005. Pre-biotic Effects of Sobo Drink on Colonization Resistance to Experimental Infection with Staphylococcus aureus 8588 in Rats. Pakistan Journal of Biological Sciences, 8: 860-866.

DOI: 10.3923/pjbs.2005.860.866


1:  Sandra, J.M. and A. Tschemia, 2002. Human studies with pro-biotics and pre-biotics: A clinical perspective. Br. J. Nutr., 87: S241-S246.
PubMed  |  

2:  Tan, F. and D.B. Rolk, 2002. Pro-biotic bacterium prevents cytokine-induced apoptosis in intestinal epithelial cells. J. Biol. Chem., 77: 50959-50965.
PubMed  |  

3:  Rachmilewitz, D., F. Kareli, K. Tkabayashi H. Tomoko and L.T. Leonor et al., 2002. Immunostimulatory DNA ameliorates experimental and spontaneous murine colitis. Gastroenterology, 122: 428-441.
Direct Link  |  

4:  Clancy, R., 2003. Immunobiotics and the pro-biotic evolution. FEMS Immunol. Med. Microbiol., 38: 9-12.
Direct Link  |  

5:  Gionchetti, P., C. Amadini, F. Rize, A. Venturi and G. Poggioli et al., 2003. Pro-biotics for the treatment of post-operative complications to following intestinal surgery. Best Pract. Res. Clin. Gastroenterol., 17: 821-831.

6:  Marteau, P.R., 2002. Pro-biotics in clinical conditions. Clin. Rev. Allergy Immounol., 22: 255-273.
PubMed  |  

7:  Kirjavainen, P.V., S.T. Salminen and E. Isolauri, 2003. Pro-biotic bacteria in the management of atopic diseases; underscoring the importance of viability. J. Pediatr. Gastroenterol. Nutr., 36: 223-227.
PubMed  |  

8:  Hamilton-Miller, J.M., 2003. The role of pro-biotics in the treatment and prevention of Helicobacter pylori infection. Int. J. Antimicrob. Agents, 22: 360-366.
PubMed  |  

9:  Vshiyama, A., K. Tanaka and Y. Aiba, 2003. Lactobacillus gasseri OL 12726 as a pro-biotic in clarithromycin-resistant Helicobacter pylori infection. J. Gastroenterol. Hepatol., 18: 986-991.

10:  Bazzocchi, G., P. Gionchetti, P.F. Almerigi, C. Amadini and M. Campieri, 2002. Intestinal microflora and oral bacteriotherapy in irritable bowel syndrome. Dig. Liver Dis., 34: 548-553.
PubMed  |  

11:  Schley, P.D. and C.J. Field, 2002. The immune-enhancing effects of dietary fibres and prebiotics. Br. J. Nutr., 87: S221-S230.
CrossRef  |  PubMed  |  Direct Link  |  

12:  Fedorak, R.N., 2004. Pro-biotics and pre-biotics in gastrointestinal disorders. Curr. Opin. Gastroenterol., 20: 146-155.
PubMed  |  

13:  Ceuilebas, A., F. Yemni, F.W. Ezzedem and T. Yardimic, 1994. Antitumoral, antibacterial and antifungal activities of Kefir and Kefir grain. Phytother. Res., 8: 78-82.

14:  Czerkinsky, C., L.A. Nnilson, H. Nygren, O. Ouchterlony and A. Tarkowski, 1983. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration septic antibody-secreting cells. J. Immunol. Methods, 65: 109-121.

15:  De Simone, C., A. Ciardi, A. Grassi, S.L. Gardini and S. Tzantzoglou et al., 1992. Effect of Bifidobacterium bifidum and Lactobacillus acidophilus on gut mucosa and peripheral blood B lymphocytes. lympnocytes. Immunopharmaco. Immunotoxicol., 14: 331-340.
PubMed  |  

16:  Isolauri, E., H. Majamaa, T. Arvola, I. Ranrala, E. Virtanen and M. Arvilom, 1993. Hactobacillus cassei strain GG reverses increased intestinal permeability induced by cow milk in suckling rats. Gastroenterology, 105: 1643-1650.

17:  Kolida, S., K. Tuday and G.R. Gibson, 2002. Pre-biotic effects of inulin and oligofructose. Br. J. Nutr., 87: S193-S197.
PubMed  |  

18:  Jeandel, C., M.C. Havrain and F. Dewcotigenies, 1996. Infectious diarrhea in the aged. Rev. Prat., 46: 184-188.
Direct Link  |  

19:  Chen, R.M., J.J. Wu, S.C. Lee, A.H. Huang and H.M. Wu, 1999. Increase in intestinal Bifidobactoerium and suppression of coliform bacteria with short term yoghurt ingestion. J. Dairy Sci., 82: 2308-2314.

20:  Thoreux, K. and D.L. Schmucker, 2001. Kefir milk enhances intestinal immunity in young but not old rats. J. Nutr., 131: 807-812.
Direct Link  |  

21:  Duke, J.A., 1987. Handbook of Medical Plants. CRC Press Inc., Cleveland, Ohio, pp: 228-229.

22:  Otemuyiwa, I.O., O.S. Falade, S.R.A. Adewusi and A. Oladipo, 2005. Effect of some herb decoctions on in vitro mineral availability from two cultivars of cowpea (Vigna unguiculata L. Walp). J. Herbal Pharmacother., 3: 327-332.
Direct Link  |  

23:  Subrania, S.S. and A.G. Hair, 1972. Flavonoids of 4 malvaceous plants. Phytochemistry, 11: 518-520.

24:  Ajani, E.O., D.A. Ameh and P.C. Onyenekwe, 1999. An investigation into the biochemical effects of the continuous consumption of Hibiscus sabdariffa calyx extracts (zoborodo) in rats. Nig. J. Nutr. Sci., 20: 7-12.

25:  White, L.A., M.C. Newman, G.L. Cromwell and M.D. Lindemann, 2002. Brewers dried yeast as a source of mannan oligosaccharides for weanling pigs. J. Anim. Sci., 80: 2619-2628.
CrossRef  |  Direct Link  |  

26:  Mukai, T., T. Asasaka, E. Sato, K. Mori, M. Matsumoto and H. Ohori, 2002. Inhibition of binding of Helicobacter pylori to the glycolipid receptors by pro-biotic Lactobacillus reuteri. FEMS Immunol. Med. Microbiol., 32: 105-110.
PubMed  |  

27:  Resta-Lenert, S. and K.E. Barrett, 2003. Live pro-biotics protect intestinal epithelial cells from the effects of infection with entero-invasive Escherichia coli (EIEC). Gut, 52: 988-997.
PubMed  |  

28:  Belley, A., K. Kelley, M. Guettke and K. Chadee, 1999. Intestinal mucins in colonization and host defense against pathogens. Am. J. Trop. Med. Hyg., 60: 10-15.
CrossRef  |  Direct Link  |  

29:  Silva, A.M., E.A. Bambara, Al. Oliveira, P.D. Souza, D.A. Gomes, E.C. Viera and J.R. Nicoli, 1999. Protective effects of bifidus milk on the experimental infection with Salmonella enteriditis sub sp. typhimuriumt in conventional and gnotobiotic mice. J. Applied Microbiol., 86: 331-336.

30:  Bauer, T.M., J. Fernandez, M. Navasa, V. Jordi and R. Juan, 2002. Failure of Lactobacillus spp. to prevent bacterial translocation in a rat model of experimental cirrhosis. J. Hepatol., 36: 501-506.
Direct Link  |  

31:  Rao, A.V., 1999. Dose-dependent effects of Inulin and Oligofructose on intestinal bifidogenesis. J. Nutr., 129: 14425-14458.

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