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Research Article

A Study on the Culture of Chlorella ellipsoidea in Various Concentrations of Unripe Tomato Juice Media

B. Mondal , M.R. Rahman , M.J. Alam , A.R. Tarafder , M.A.B. Habib and M.A. Khaleque
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The experiment was conducted to evaluate the performance of organic nutrients of Unripe Tomato Juice (URTJ) added with 0.2 g L-1 urea and Bold Basal Medium (BBM) as standard, for growing of C. ellipsoidea in laboratory condition for three months. C. ellipsoidea was cultured four different concentrations such as 0.7, 1.4, 2.8 and 4.15 g L-1 URTJ with added 0.2 g L-1 urea and Bold Basal Medium for a period of 16 days. The initial cell density of C. elliposidea was 2.90x mL-1, which attained a maximum cell density of 108.03x mL-1 in BBM followed by 75.24x, 69.67x, 54.80xand 27.35x mL-1 in 2.8, 1.4, 0.7 and 4.15 g L-1 of URTJ media with added 0.2 g L-1 urea, respectively on the 12th day of culture. Similar trend was observed in case of optical density of C. ellipsoidea. The proximate composition of C. ellipsoidea cultured in URTJ media was analyzed and found that the ranges of crude protein, crude lipid, ash and moisture ranged from 22.39-39.91%, 3.66-5.19%, 10.10-11.11% and 8.15-9.45%, respectively. Results showed that growth of C. ellipsoidea was significantly (p<0.05) higher in the concentrations of 2.8 g L-1 of URTJ with added 0.2 g L-1 urea than other concentrations (0.7, 1.4 and 4.15 g L-1) of URTJ with added urea at the temperature of 29.04°C, dissolved oxygen of 5.71 mg L-1, pH of 8.08 and light intensity of 1583 luxm-2s-1

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  How to cite this article:

B. Mondal , M.R. Rahman , M.J. Alam , A.R. Tarafder , M.A.B. Habib and M.A. Khaleque , 2005. A Study on the Culture of Chlorella ellipsoidea in Various Concentrations of Unripe Tomato Juice Media. Pakistan Journal of Biological Sciences, 8: 823-828.

DOI: 10.3923/pjbs.2005.823.828


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