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Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 5 | Page No.: 687-690
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Research Article

Phytotoxicity of Lead (Pb) to SDS-PAGE Protein Profile in Root Nodules of Faba Bean (Vicia faba L.) Plants

Mohamed S. Beltagi

ABSTRACT

The present study was carried out to characterize and assess possible changes that might take place in the electrophoretic protein patterns involved in the N2-fixing root nodules of Vicia faba L.(faba bean) in response to lead (Pb) toxicity. The banding patterns of the SDS-PAGE protein profile in root nodules revealed both qualitative and quantitative changes. Lead treatments (PbO), either applied foliar or in soil, inhibited the number of polypeptides synthesized in the root nodules. The band intensity of the large subunit (240 kDa) of nitrogenase enzyme was greatly inhibited by all PbO treatments. Moreover, the synthesis of the small subunits (52 to 73 kDa) of nitrogenase was completely inhibited by both soil applications (500 and 2000 ppm) of PbO. The foliar applications (500 and 2000 ppm) of PbO greatly stimulated a 23 kDa protein (from 4.79 to 13.1 and 19.4%, respectively). This protein was also stimulated (only 2-folds) in the root nodules by PbO soil treatments. In conclusion, the reduced productivity of Vicia faba L. crop under Pb stress could be attributed to the impairment of N2-fixing enzymes.
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