Subscribe Now Subscribe Today
Research Article
 

Insecticidal Activity of Carbaryl and its Mixture with Piperonylbutoxide Against the Red Palm Weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) and their Effects on Acetylcholinesterase Activity



DiefAlla H. Al-Rajhy , Hamdy I. Hussein and Abdul-Monem A. Al-Shawaf
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

The insecticidal activity of carbaryl alone and its mixture with Piperonylbutoxide (PBO) was tested against males, females and larvae of the Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier), by both the feeding and the topical application methods. In addition, the Acetylcholinesterase (AChE) activity, the in vitro effect of carbaryl alone and the in vivo effects of carbaryl, PBO and their mixture on the AChE activity were estimated in RPW. A modification was carried out to enable estimation of AChE activity in this destructive pest. Tested materials were more toxic by feeding than by topical application and larvae were more susceptible than adults. In case of food treatment, the LC50 values of carbaryl alone were 1.183, 1.43 and 0.0367% for males, females and larvae, respectively. PBO had great synergistic effect on carbaryl toxicity, the LC50 values for carbaryl+PBO were 0.0155, 0.0253 and 0.00556% against males, females and larvae, respectively. The topical LD50 values for carbaryl and carbaryl+PBO against larvae were 27.1 and 14.4 μg/larva, respectively. The inhibition percentages of AChE activity in larvae fed on carbaryl and (carbaryl+PBO)-treated sugarcane were 0.8 and 64.4%, respectively. The results indicated the great role of oxidases in the reduction of carbaryl toxicity and their inhibition by PBO.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

DiefAlla H. Al-Rajhy , Hamdy I. Hussein and Abdul-Monem A. Al-Shawaf , 2005. Insecticidal Activity of Carbaryl and its Mixture with Piperonylbutoxide Against the Red Palm Weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) and their Effects on Acetylcholinesterase Activity. Pakistan Journal of Biological Sciences, 8: 679-682.

DOI: 10.3923/pjbs.2005.679.682

URL: https://scialert.net/abstract/?doi=pjbs.2005.679.682

REFERENCES
1:  Cox, M.L., 1993. Red palm weevil, rhynchophorus ferrugineus in Egypt. FAO Plant Prot. Bull., 41: 30-31.

2:  Abraham, V.A., M.A. Al-Shuaibi, J.R. Faleiro, R.A. Abuzuhairah and P.S.P.V. Vidyasagar, 1998. An integrated management approach for red date palm weevil, Rhynchophorus ferrugineus Oliv, a key pest of date palm in Middle East. Sultan Qabus Univ. J. Sci. Res. Agric. Sci., 3: 77-84.

3:  Murphy, S.T. and B.R. Briscoe, 1999. The red palm weevil as an alien invasive: Biology and the prospects of biological control as component of IPM. Biocontrol News Inform., 20: 35N-46N.
Direct Link  |  

4:  Kurian, C. and K. Mathen, 1971. Red palm weevil-hidden enemy of coconut palm. Indian Farm., 21: 29-31.

5:  Mathen, K. and K.C. Kurian, 1970. Sevin controls red palm weevil at low cost. Coconut Bull., 1: 7-8.

6:  Subba-Rao, P.V., T.R. Subramaniam and E.V. Abraham, 1973. Control of the red palm weevil on coconut. J. Plant Crops, 1: 26-27.

7:  Abraham, V.A., K.M.A. Koya and C. Kurian, 1975. Evaluation of seven insecticides for control of red palm weevil Rhynchophorus ferrugineus F. J. Plant. Crops, 3: 71-72.

8:  Ganeswara, R.A., K. Laxminarayana and R.P. Ramamohana, 1980. Administration of systemic insecticide through root-A new method of control of Red Palm Weevil, Rhynchophorus ferrugineus Fab in coconut. Indian Coconet J., 11: 5-6.
Direct Link  |  

9:  Sathiamma, B., V.A. Abraham and C. Kurian, 1982. Integrated pest management of the major pests of coconut. Indian Coconut J., 12: 27-29.

10:  Rao, A.G., R.P. Ramamohana, R.T. Ramamohana and K. Lakshminarayana, 1989. Studies on the effect of root feeding of systemic insecticides in the control of Red Palm Weevil, Rhynchophorus ferrugineus Fab in coconut. Indian Coconut J., 19: 12-16.

11:  Rajan, P. and C.P.R. Nair, 1997. Red palm weevil-the tissue borer of coconut palm. Indian Coconut J., 27: 2-4.

12:  Ajlan, A.M., M.S. Shawir, M.M. Abo-el-Saad, M.A. Rezk and K.S. Abdulsalam, 2000. Laboratory evaluation of certain organophosphorus insecticides against the Red Palm Weevil, Rhynchophorus ferrugineus (Olivier). J. King Faisal Univ. Applied Sci., 1: 15-26.

13:  El-Ezaby, F., 1997. A biological in vitro study on the red indian date palm weevil. Arab J. Plant Prot., 15: 84-87.

14:  Finney, D.J., 1971. Probit Analysis. 3rd Edn., Cambridge University Press, London, pp: 318.

15:  Ellman, G.L., K.D. Courtney, V. Andres Jr. and R.M. Featherstone, 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 7: 88-95.
CrossRef  |  Direct Link  |  

16:  Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
PubMed  |  Direct Link  |  

17:  Solomon, S.S., L.I. Shao-Nan and F. de Fan, 2000. In vitro inhibition and recovery of brain acetylcholinesterase in Topmouth Gudgeon (Pseudorasobora parva) following exposure to fenitrothion. J. Zhejiang Univ. Sci., 1: 448-458.

©  2021 Science Alert. All Rights Reserved