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Research Article

Effect of Heavy Metals on the Developmental Stages of Ovule and Embryonic Sac in Euphorbia cheiradenia

Behrouz E. Malayeri , Abdolkarim Chehregani , Fariba Mohsenzade and Roghayeh Golmohammadi
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The aim of this research was to elucidate some microscopic effects of heavy metals on the developmental stages of ovules in plants. We chose a large area in the near Malayer city as a polluted area. In this region amount of heavy metals is several times more than non-polluted area. Flowers and young pods removed from plants. The specimens fixed in FAA70 stored in 70% ethanol, embedded in paraffin and sectioned at 8 μm with microtome. Staining was carried out with Hematoxylin and developmental stages were compared in plants that collected from polluted and non-polluted area. The results of this research showed that heavy metals can affect some abnormality in during of ovule developmental process, in plants that collected from polluted area. These abnormalities include changes of ovule shape, quick degradation of embryonic sac`s cell, quick growth of integuments, an increase in embryonic sac cytoplasm concentration, irregularity and even stopping the nuclear envelope formation. Finally reduction in number of ovules were seen in polluted plants.

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  How to cite this article:

Behrouz E. Malayeri , Abdolkarim Chehregani , Fariba Mohsenzade and Roghayeh Golmohammadi , 2005. Effect of Heavy Metals on the Developmental Stages of Ovule and Embryonic Sac in Euphorbia cheiradenia . Pakistan Journal of Biological Sciences, 8: 622-625.

DOI: 10.3923/pjbs.2005.622.625


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