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Research Article
 

Effect of Sodium Chloride and Glycine Betaine on Growth and Pectate Lyase Production by Erwinia carotovora subsp. carotovora



Hoda H. El-Hendawy and Mohamed E. Osman
 
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ABSTRACT

E. carotovora subsp. carotovora was grown in YS medium containing different NaCl concentrations. The growth was stimulated, reduced and totally inhibited in presence of 1.5, 3 and 6% NaCl, respectively. Addition of glycine betaine increased the growth in presence of NaCl, but not in its absence, in comparison with the control. Assays of extracellular and cell associated pectate lyase produced by E. carotovora in NaPP-YS medium containing 1.5 and 3% NaCl revealed that the extracellular PL activity was inhibited in presence of NaCl relative to the control. On the other hand, the level of the cell associated enzyme was increased with increasing NaCl concentration. When addition of glycine betaine to the growth medium, the activity of the extracellular PL was increased even in the control medium devoid of NaCl. Also, glycine betaine released the intracellular PL, this was evidenced by the reduction of the cell associated PL activity in its presence compared with the control (absence of glycine betaine).

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  How to cite this article:

Hoda H. El-Hendawy and Mohamed E. Osman , 2005. Effect of Sodium Chloride and Glycine Betaine on Growth and Pectate Lyase Production by Erwinia carotovora subsp. carotovora. Pakistan Journal of Biological Sciences, 8: 330-334.

DOI: 10.3923/pjbs.2005.330.334

URL: https://scialert.net/abstract/?doi=pjbs.2005.330.334

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