Submit Manuscript

Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 11 | Page No.: 1614-1619
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Research Article

Antibiogram and Plasmid Profile Analysis of Isolated Poultry Salmonella of Bangladesh

M.F.R. Khan, M.B. Rahman, M.S.R. Khan, K.H.M.N.H. Nazir and M. Rahman

ABSTRACT

Antibiogram study and plasmid profile analyses were conducted to find out the correlation of the recently isolated Salmonella organisms of Bangladesh. Antibiogram study revealed that the isolates were highly sensitive to Ciprofloxacin, Cephalexin and Kanamycin. In the plasmid profile analysis it was observed that out of 4 isolates of Mymensingh district (M1 to M4), 3 isolates showed 3 plasmid bands in each and 1 isolate showed 4 plasmid bands. The estimated size of the plasmid bands of M1, M2, M3 and M4 were (33.5, 24.50, 4.0, 3.0); (33.5, 3.0, 1.0); (33.5, 3.0, 1.0) and (5.0, 4.0, 1.8) kb, respectively. On the other hand among the isolates of Feni district (F1 to F9), 6 isolates (F4, F5, F6, F7, F8 and F9) showed 5 number of plasmid bands in each and the estimated plasmid band size were same (33.5, 5.0, 4.0, 2.0, 1.8). The plasmid bands of other 3 isolates (F1, F2 and F 3) were found to be different and the estimated size were recorded as 4 (33.5, 5.0, 1.6, 1.5); 3 (33.5, 1.6, 1.5) and 1 (0.7), respectively. Among the isolates of Dhaka district (D1 to D11); no plasmid band was found to be detected in 3 isolates (D2, D5 and D6). The number of plasmid bands of other isolates of D1, D3, D4, D7, D8, D9, D10 and D11 were 4 and the estimated size were determined as (33.5, 15.0, 2.0, 1.0); 5 (33.5, 15.0, 3.0, 2.0, 1.0); 5 (33.5, 5.0, 4.0, 1.8, 1.3); 3 (33.5, 3.0, 1.0); 3 (33.5, 3.0, 1.0); 1(33.5); 2 (33.5, 3.0) and 2 (33.5, 4.0) kb, respectively. All these isolates were tested for antibiotic sensitivity against 8 commonly used antibiotics belonging to different groups. From the antibiogram study it was revealed that all the isolates of Mymensingh and Dhaka district were 100% resistant to Cloxacillin and among the isolates of Feni district 100% were resistant to Erythromycin and Cloxacillin. Plasmid profile analysis of the isolated Salmonella organisms revealed that the isolates carrying multiple plasmids which might be the cause of various degree of antibiotic resistance.
PDF References Citation

Search

REFERENCES

  1. Bhattacharjee, P.S., R.L. Kundu, J.U. Mazumder, E. Hossain and A.H. Miah, 1996. A retrospective analysis of chicken diseases diagnosed at the central disease investigation laboratory, Dhaka, Bangladesh. Bangladesh Vet. J., 30: 105-113.
  2. Yun, G.R., Y.J. Lee, K.S. Kim and R.B. Tak, 2003. Biochemical characteristics and plasmid profiles of Salmonella isolated from wild birds in Daegu area. Kor. J. Vet. Public Health, 27: 59-67.
  3. Anzai, T., Y. Kuwamoto, R. Wada, M. Akiba, T. Ohya and T. Sameshima, 2003. Virulence and other biological properties of Salmonella typhimurium isolated from foals in Japan from 1981 until 1996. J. Equine Sci., 14: 87-92.
    Direct Link
  4. Buxton, A. and G. Fraser, 1977. Animal Microbiology. Blackwell Scientific Publications, Oxford, London, Edinburg, Melbourne pp:85-86.
  5. Cheesbrough, M., 1985. Antimicrobial Sensitivity Testing. In: Medical Laboratory Manual for Tropical Countries, Vol. 2: Microbiology, Cheesbrough, M. (Ed.). 1st Edn., Tropical Health Technology and Butterworth-Heinemann Ltd., Oxford, UK., pp: 196-205.
  6. Sung, M., K. Kim, R. Tak, M.S. Sung, K.S. Kim and R.B. Tak, 2002. Contamination of Salmonella in a chicken slaughterhouse in Gyeongbuk province. Kor. J. Vet. Public Health, 26: 195-205.
  7. Roy, P., A.S. Dhillon, L.H. Lauerman, D.M. Schaberg, D. Bandli and S. Johnson, 2002. Results of Salmonella sp. Isolation from poultry products, poultry, poultry environment and other characteristics. Avian Dis., 46: 17-24.
    Direct Link
  8. Lee, D.S., T.W. Hahn and D.S. Lee, 2000. Virulence, plasmid profiles and antibiotic susceptibility of Salmonella gallinarumisolated from chickens in Korea. Kor. J. Vet. Public Health, 24: 49-57.
  9. Rahman, H., 2002. Some aspects of molecular epidemiology and characterisation of Salmonella typhimurium isolated from man and animals. Ind. J. Med. Res., 115: 108-112.
    Direct Link
  10. Bakshi, C.S., V.P. Singh, M. Malik, R.K. Singh and B. Sharma, 2003. 55 kb plasmid and virulence associated genes are positively correlated with Salmonella enteritidis pathogenecity in mice and chickens. Vet. Res. Commun., 27: 425-432.
    Direct Link
  11. Sarker, S.K., 2004. Epidemiological study of Salmonella and Mycoplasma infection in selected model breeder poultry farms of Bangladesh. M.S. Thesis, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  12. Sambrook, J. and D.W. Russel, 2001. Molecular Cloning a Laboratory Manual. Cold Spring labor Laboratory Press, New York, pp: 32-34.
  13. Maniatis, T.E., R.F. Fritsch and J. Sambrook, 1983. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, Cold Spring labor Laboratory Press, New York.

Search

Related Articles

Leave a Comment

Your email address will not be published. Required fields are marked *