ABSTRACT
In the present study thin-layer impedimetric biosensors were developed for the free-label immunoassay of Fatty Acid Binding Protein (FABP). This test based on antibody recognition was performed within a thin-layer detection cell between two pieces of conducting glass. Antibodies were immobilized by physical adsorption. The biosensor exhibited obvious change of impedimetric signals due to the adsorbed antibody and subsequently captured antigen. No label of enzyme or aurum nanoparticle is needed. The given equivalent circuit is consonant well with the impedimetric results.
PDF References Citation
How to cite this article
Yu-Qing Miao, 2005. Thin-layer Impedimetric Biosensors for the Free-label Immunoassay. Pakistan Journal of Biological Sciences, 8: 1544-1547.
DOI: 10.3923/pjbs.2005.1544.1547
URL: https://scialert.net/abstract/?doi=pjbs.2005.1544.1547
DOI: 10.3923/pjbs.2005.1544.1547
URL: https://scialert.net/abstract/?doi=pjbs.2005.1544.1547
REFERENCES
- Liu, H., G.H. Dong, B. Xu, Y. Shen and H. Jing, 2005. Heart fatty acid binding protein in the rapid evaluation of myocardial damage following valve replacement surgery. Clinica Chimica Acta, 356: 147-153.
PubMedDirect Link - Pagani, F., R. Bonora, G. Bonetti and M. Panteghini, 2000. Evaluation of a sandwich enzyme-linked immunosorbent assay for the measurement of serum heart fatty acid-binding protein. Ann. Clin. Biochem., 39: 404-405.
Direct Link - Seino, Y., K. Ogata, T. Takano, J. Ishii and H. Hishida et al., 2003. Use of a whole blood rapid panel test for heart-type fatty acid-binding protein in patients with acute chest pain: Comparison with rapid troponin T and myoglobin tests. Am. J. Med., 115: 185-190.
PubMedDirect Link - Van der Voort, D., M.M.A.L. Pelsers, J. Korf, W.T. Hermens and J.F.C. Glatz, 2003. Development of a displacement immunoassay for human heart-type fatty acid-binding protein in plasma: The basic conditions. Biosensors Bioelectronics, 19: 465-471.
PubMedDirect Link - Van der Voort, D., M.M. Pelsers, J. Korf, W.T. Hermens and J.F. Glatz, 2004. A continuous displacement immunoassay for human heart-type fatty acid-binding protein in plasma. J. Immunol. Methods., 295: 1-8.
CrossRef - Ghindilis, A.L., P. Atanasov, M. Wilkins and E. Wilkins, 1998. Immunosensors: Electrochemical sensing and other engineering approaches. Biosensors Bioelectron., 13: 113-131.
CrossRef - Luppa, P.B., L.J. Sokoll and D.W. Chan, 2001. Immunosensors-principles and applications to clinical chemistry. Clinica Chimica Acta, 314: 1-26.
CrossRef - Ma, J., Y.M. Chu, J. Di, S.C. Liu, H.N. Li, J. Feng and Y.X. Ci, 1999. An electrochemical impedance immunoanalytical method for detecting immunological interaction of human mammary tumor associated glycoprotein and its monoclonal antibody. Electrochem. Commun., 1: 425-428.
CrossRef - Kharitonov, A., L. Alfonta, E. Katz and I. Willner, 2000. Probing of bioaffinity interactions at interfaces using impedance spectroscopy and chronopotentiometry. J. Electroanal. Chem., 487: 133-141.
CrossRef - Lillie, G., P. Payne and P. Vadgama, 2001. Electrochemical impedance spectroscopy as a platform for reagentless bioaffinity sensing. Sensors Actuators B, 78: 249-256.
CrossRef - Querghi, O., A. Touhami, N. Jaffrezic-Renault, C. Martelet, H.B. Ouada and S. Cosnier, 2002. Impedimetric immunosensors using avidin-biotin for antibody immobilization. Bioelectrochemistry, 56: 131-133.
CrossRef - Bott, A.W., 2001. Electrochemical techniques for the characterization of redox polymers. Curr. Separat., 19: 71-75.
Direct Link - Oliveira-Brett, A.M., L.A. Silva and C.M.A. Brett, 2002. Adsorption of guanine, guanosine and adenine at electrodes studied by differential pulse voltammetry and electrochemical impedance. Langmuir, 18: 2326-2330.
Direct Link - Omanovic, S. and S.G. Roscoe, 1999. Electrochemical studies of the adsorption behavior of bovine serum albumin on stainless steel. Langmuir, 15: 8315-8321.
CrossRefDirect Link - Boubour, E. and R.B. Lennox, 2000. Insulating properties of self-assembled monolayers monitored by impedance spectroscopy. Langmuir, 16: 4222-4228.
CrossRef