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Effect of Probiotic Inclusion in Different Levels of Barley Substitution for Corn Diets on Egg Quality and Laying Hen`s Performance

A.H. Mahdavi , H.R. Rahmani , J. Pourreza and M.A. Edriss

The purpose of this study was to investigate the effect of probiotic (0, 400, 1000 and 2000 g Bioplus 2B ton-1 feed providing 0, 1.28 x 106, 3.2 x 106 and 4.6 x 106 cfu g-1 feed concentration) in different levels of barley substitution for corn diets (0, 50 and 100%) on laying hen`s performance, egg quality, blood factors and histological changes in duodenum. Evaluated traits were egg production, egg weight, egg mass, feed consumption, feed conversion ratio, shell thickness, shell hardness, Haugh unit, egg cholesterol, plasma cholesterol, plasma triglyceride and histological changes of duodenum. Using different levels of substitution of barley for corn showed highly significant decrease (p<0.01) in feed consumption and feed conversion ratio, highly significant increase (p<0.01) in goblet cell numbers and epithelium surface folds of villus, significant increase (p<0.05) in egg weight and damaged apical cells of villus, without any effect on other traits. Although, using the different levels of probiotic caused highly significant increase (p<0.01) in goblet cell numbers, significant increase (p<0.05) in feed consumption, feed conversion ratio and destroying apical cells of villus and significant decrease (p<0.05) in plasma cholesterol and triglyceride, but it had no effects on other traits. Consumption of probiotic alone had no effect on egg cholesterol (mg g-1 of yolk), but caused significant decrease (p<0.05) in egg cholesterol (mg g-1 of yolk) among fourth level of probiotic and another levels, in corn based diets. Interaction of different percentages of substitution of barley for corn and probiotic levels had highly significant effect (p<0.01) on feed consumption, feed conversion ratio and egg cholesterol (mg g-1 of yolk) and significant effect (p<0.05) on Hough unit.

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A.H. Mahdavi , H.R. Rahmani , J. Pourreza and M.A. Edriss , 2005. Effect of Probiotic Inclusion in Different Levels of Barley Substitution for Corn Diets on Egg Quality and Laying Hen`s Performance. Pakistan Journal of Biological Sciences, 8: 1521-1528.

DOI: 10.3923/pjbs.2005.1521.1528


Abdulrahim, S.M., M.S.Y. Haddadin and A.R. Hashlamoun, 1996. The influence of Lactobacillus acidophilus and bacitracin on layer performance and cholesterol of plasma and egg yolk. Br. Poult. Sci., 37: 341-346.

Al-Bustany, Z. and K. Elwinger, 1988. Whole grains, unprocessed rapeseed and beat-glucanse in diet for laying hens. Sweden J. Agric., 18: 31-40.

Anonymous, 1999. SAS Users Guide. SAS Institute Inc., Cary, NC..

Beyer, R.S. and L.S. Jensen, 1993. Tissue and egg cholesterol concentration of laying hen fed high-protein barley flour, α-tocotrienol and cholesterol. Poult. Sci., 72: 1339-1348.

Blottieres, H.M., M. Champ, C. Hoebler, C. Michel and C. Cherbut, 1999. Production and digestive effects of short chain fatty acids. Sci. Aliment., 19: 269-290.

Blum, S., D. Haller, A. Pfeifer and E.J. Schiffrin, 2002. Probiotics and immune response. Clin. Rev. All Immunol., 22: 287-309.

Cerniglia, G.J., A.C. Goodling and J.A. Hebert, 1983. The response of layers to feeding Lactobacillus fermentation product. Poult. Sci., 62: 1399-1399.

Chen, Y.C. and T.C. Chen, 2003. Effects of commercial probiotic or prebiotic supplementation on production, size and quality of hen's egg. Poult. Sci., 82: 330-330.

Coates, M.E. and R. Fuller, 1977. The Genotobiotic Animal in the Study of Gut Microbiology. In: Microbial Ecology of the Gut, Clarke, R.T.J. and T. Bauchop (Eds.). Academic Press, London, pp: 311-346.

Collington, G.K., D.S. Parker and D.G. Armsterong, 1990. The influence of either an antibiotic or a probiotic in the diet on the development of digestive enzyme activity in the pig. Br. J. Nutr., 64: 59-70.

Damron, B.L., A.R. Eldered and R.H. Harms, 1976. An improvement in interior egg quality by the feeding of brewers dried grains. Poult. Sci., 55: 1365-1366.

Davidson, M.H., L.D. Dugan, J.H. Burns, J. Bova, K. Story and K.B. Drennan, 1991. The hypocholesterolemic effects of β-glucan in oat meal and oat brane: A dose-controlled study. J. Am. Med. Assoc., 265: 1833-1839.

Dunham, H.J., C. William, F.W. Edens, I.A. Casas and W.J. Dobrogosz, 1993. Lactobacillus reuteri immunomodulation of stressor associated disease in newly hatched chickens and turkeys. Poult. Sci., 72: 103-103.

Fioramonti, J., V. Theodorou and L. Bueno, 2003. Probiotics: What are they? What are their effects on gut physiology? Best Pract. Res. Clin. Gastroenterol., 17: 711-724.
CrossRef  |  Direct Link  |  

Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
PubMed  |  Direct Link  |  

Fuller, R., 1989. Probiotics in man and animals. J. Applied Bacteriol., 66: 365-378.
CrossRef  |  PubMed  |  Direct Link  |  

Gilliland, S.E. and S.H. Kim, 1984. Effect of viable starter culture bacteria in yogurt on lactose utilization in humans. J. Dairy Sci., 97: 1-6.

Goodling, A.C., 1987. Production performance of white leghorn layers fed lactobacillus fermentation products. J. Poult. Sci., 66: 480-486.

Haddadin, M.S.Y., S.M. Abdulrahim, E.A.R. Hashlamoun and R.K. Robinson, 1996. The effects of Lactobacillus acidophilus on the production and chemical composition of hen's eggs. Poult. Sci., 75: 491-494.

Jensen, L.S. and D.V. Maurice, 1978. Effect of chromium and corn fermentation solubles on interior egg quality. Poult. Sci., 57: 1147-1151.

Jensen, L.S., C.H. Chang and S.P. Wilson, 1978. Interior egg quality: Improvement by distillers feeds and trace elements. Poult. Sci., 57: 648-654.
CrossRef  |  Direct Link  |  

Jeroch, H. and S. Danicke, 1995. Barley in poultry feeding: A review. World. Poult. Sci., 51: 271-291.

Klopfenestein, C.F. and R.C. Hoseney, 1987. Cholesterol-lowering effect of β-glucan enriched bread. Nutr. Rep. Intl., 36: 1091-1098.

Leeson, S. and J.D. Summers, 1997. Commercial Poultry Nutrition. University Books, Ontario, Canada.

Marks, H.L. and H.W. Washburn, 1991. Plasma and yolk cholesterol levels in Japanese quail divergently selected for plasma cholesterol response to adrenocoticotropin. Poult. Sci., 70: 429-433.

McIntosh, G.H., J. Whyte, R. McArthur and P.J. Nestel, 1991. Barley and wheat foods: Influence on plasma cholesterol concentrations in hypercholesterolemic men. Am. J. Clin. Nutr., 53: 1205-1209.
PubMed  |  Direct Link  |  

Miles, R.D., A.S. Arofa, R.H. Harms, C.W. Carlson, B.L. Reid and J.S. Crawford, 1981. Effects of a living non freeze-dried Lactobacillus acidophilus culture on performance, egg quality and gut microflora in commercial layers. Poult. Sci., 60: 993-1004.

Mohan, B., R. Kadirvel, M. Bhaskaran and A. Natarajan, 1995. Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers. Br. Poult. Sci., 36: 799-803.
CrossRef  |  Direct Link  |  

Montagne, L., J.R. Pluske and D.J. Hampson, 2003. A review of interactions between dietary fibre and the intestinal mucosa and their consequences on digestive health in young non-ruminant animals. Anim. Feed. Sci. Technol., 108: 95-117.
CrossRef  |  Direct Link  |  

Nahashon, S.N., H.S. Nakaue and L.W. Mirosh, 1992. Effect of direct-fed microbials on nutrient retention and production parameters of laying pullets. Poult. Sci., 71: 111-111.

Nahashon, S.N., H.S. Nakaue and L.W. Mirosh, 1996. Performance of single comb white leghorn fed a diet supplemented with a live microbial during the growth and egg laying phases. Anim. Feed. Sci. Technol., 57: 25-38.
CrossRef  |  Direct Link  |  

Nahashon, S.N., H.S. Nakaue and L.W. Mirsh, 1993. Effect of direct fed microbials on nutrient retention and productive parameters of single comb white leghorn pullets. Poult. Sci., 72: 87-87.

Prins, R.A., 1977. Biochemical Activities of Gut Microorganisms. In: Microbial Ecology of the Gut, Clarke, R.T.J. and T. Hauchop (Eds.). Academic Press, London, pp: 73-183.

Qureshi, A.A., W.C. Burger, N. Prentice, H.R. Bird and M.L. Sunde, 1980. Suppression of cholesterol and stimulation of fatty acid biosynthesis in chicken livers by dietary cereals supplemented with culture filtrate of Trichoderia viride. J. Nutr., 110: 1014-1022.

Rolfe, R.D., 2000. The role of probiotic cultures in the control of gastrointestinal health. J. Nutr., 130: 396S-402S.
Direct Link  |  

Saarela, M., G. Mogensen, R. Fonden, J. Matto and T. Mattila-Sandholm, 2000. Probiotic bacteria: Safety, functional and technological properties. J. Biotechnol., 84: 197-215.
CrossRef  |  Direct Link  |  

Simon, O., 1998. The mode of action of NSP hydrolyzing enzymes in the gastrointestinal tract. J. Anim. Feed Sci., 7: 115-123.

Story, J.A. and E.J. Furumoto, 1990. Dietary Fiber and Bile Acid Metabolism. In: Health Effects, Kritchevsky, D., D. Richevsky, C. Bonfield and J.W. Anderson (Eds.). Plenum Press, New York, pp: 364-365.

Sutton, C.D., W.M. Nuir and G.E. Mitchell Jr., 1984. Cholesterol metabolism in laying hen as influenced by dietary cholesterol, caloric intake and genotype. Poult. Sci., 63: 972-980.
CrossRef  |  

Toms, C. and F. Powrie, 2001. Control of intestinal inflammation by regulatory T cells. Microb. Infect., 3: 929-935.
Direct Link  |  

Tortuero, F. and E. Fernandez, 1995. Effect of inclusion of microbial culture in barly-based diets fed to laying hens. Anim. Feed Sci. Technol., 53: 255-265.
Direct Link  |  

Washburn, K.W. and D.F. Nix, 1974. A rapid technique for extraction of yolk cholesterol. Poult. Sci., 53: 1118-1122.

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