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Research Article

Effect of Aqueous Extract of Prasium majus`s L. Leaves on Water and Electrolytes Transport in Rat Intestine

F. Khemiss , D. Saidane and A.A. Moshtaghie
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The major aim of this investigation was to examine the effect of aqueous extract of Prasium majus`s L. leaves (AEPML) on intestinal influx of water and electrolytes by Everted Gut Sac (EGS). Four segments of rat intestine were detected and used (duodenum, jejunum, ileum and colon). The extract of the plant was used with two concentrations,8 and  16 g L-1, respectively. The results obtained showed that AEPML provoke; firstly  an  inhibition  of  water  secretion by   different  parts  of  EGS, but decrease was only significant at the ileum (p<0.05).Secondly electrolytes fluxes were also sensitive to the addition of AEPML.On  the  other hand  for  Na+ influx,we noted an inhibition of the secretion at the duodenum and the colon (p < 0.01) with the dose 8 g L-1, while for Cl-, influx this inhibition was only registered at the colon (p<0.01) with the dose 8 g L-1. For K+ influx, AEPML induced rather a significant reabsorption at the duodenum, the jejunum and the colon  (p < 0.01) hile at the ileum the reabsorption increased. This was only significant with the dose 16 g L-1.It may be concluding that the heterogeneity of the gastrointestinal tract in response to numerous factors which are able to affect homeostasis of water and electrolytes. AEPML might have an antisecretory function on water Na+ and Cl- and an absorptive function on K+.

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  How to cite this article:

F. Khemiss , D. Saidane and A.A. Moshtaghie , 2005. Effect of Aqueous Extract of Prasium majus`s L. Leaves on Water and Electrolytes Transport in Rat Intestine. Pakistan Journal of Biological Sciences, 8: 1424-1429.

DOI: 10.3923/pjbs.2005.1424.1429


1:  Mayol, J.A. and J.A.F. Represa, 1998. Chloride secretion in the intestinal epithelium channels ions and intracellular signaling. Rev. Esp. Enferm. Dig., 90: 714-721.
PubMed  |  

2:  Larsen, E.H., J.B. Sorensen and J.N. Sorensen, 2000. A mathematical model of solute coupled water transport in toad intestine incorporating recirculation of the actively transported solute. J. Gen. Physiol., 116: 101-124.
Direct Link  |  

3:  Kreydiyyeh, S.I., J. Usta and R. Copti, 2000. Effect of cinnamon, clove and some of their constituents on the Na+Kss+ ATPase activity and alanine absorption in the rat jejunum. Food Chem. Toxicol., 38: 755-762.

4:  Meerveld, B.G.V., K. Tyler, T. Kuge and N. Ogata, 1999. Anti diarrhoeal effects of seirogan in the rat small intestine and colon examined in vitro. Aliment Pharmacol. Ther., 13: 97-102.
Direct Link  |  

5:  Floch, E.L., 1983. Contribution a Une Etude Ethnobotanique de La Flore Tunisienne. Imprimerie Officielle De La Republique Tunisienne, Tunis, pp: 208.

6:  Wilson, T.H. and G. Wiseman, 1954. The use of sacs everted of small intestine for study of transference of substance from mucosal to serol surface. J. Physiol., 123: 116-125.

7:  Moshtaghie, A.A. and M. Taher, 1993. Aluminium interference with iron absorption by everted gut sac. J. Islamic Acad. Sci., 6: 277-281.

8:  Issa, A.R.C., A. Gharzouli, G. Charpin, M.D. Vagne and D. Pansu, 1992. Comparison of VIP induced electrolyte secretion at three levels in rat small intestine. Reprod. Nutr. Dev., 32: 37-45.
Direct Link  |  

9:  Issa, A.R.C., G. Charpin, C. Dumas, P. Nicol, D. Pansu and M.D. Vagne, 1993. Comparative duodenal jejunal and ileal reponses to luminal saline load. Reprod. Nutr. Dev., 33: 151-164.
PubMed  |  

10:  Charpin, G., C. Dumas, A. Baz, M.D. Vagne and D. Pansu, 1993. Comparison of hydroelectrolytic exchange characterized by calculation of saturable fluxes at three levels of the rat intestine. Arch. Int. Physiol. Biochem. Biophys., 101: 377-386.

11:  Charpin, G., A.R.C. Issa, H. Guignard, G. Jourdan, C. Dumas, D. Panssu and M.D. Vagne, 1992. Effect of sorbin on duodenal absorption of water and electrolytes in the rat. Gastroenterology, 103: 1568-1573.
Direct Link  |  

12:  Ma, T. and A.S. Verkman, 1999. Aquaporin water channels in gastointestinal physiology. J. Physiol., 517: 317-326.

13:  Koyama, Y., T. Yamamoto, T. Tani, K. Nihei and I. Kihara et al., 1999. Expression and localisation of aquaporins in rat gastrointestinal tract. Am. J. Physiol., 276: 621-627.
Direct Link  |  

14:  Fioriti, P., J.M. Burgos, M.F. Miyakawa, M. Rivas and C. Ibarra et al., 2000. Effect of shigha toxin 2 on water and ion transport in human colon in vitro. Dig. Dis. Sci., 45: 480-486.
Direct Link  |  

15:  Krishnan, S., B.S. Ramakrishna, G.T. Keusch, D.W. Acheson, A.B. Pulimood and M. Mathan, 1999. Effect of shiga toxin on NaCl transport in rat distal colon. Scand. J. Gastroenterol., 34: 777-783.

16:  Turvill, J.L., R.A. Wapnir, M.A. Wingertzahn, S. Teichberg and M.J. Farthing, 2000. Cholera toxin induced secretion in rat is reduced by a soluble fiber gum Arabic. Dig. Dis. Sci., 45: 946-951.

17:  Grondahl, M.L., J.E. Thorboll, M.B. Hansen and E. Skadhauge, 1998. Regional differences in the effect of cholera toxin and enterotoxigenic E. coli infection on electrolyte and fluid transport in the porcine small intestine. Zentralbl. Veternarmed., 45: 369-381.

18:  Farack, U.M., A. Asher, B. Elsenhans, B.S. Luckenga and R. Gerzer, 2000. Effect of loperamide on mucosal guanylin cyclase activity in rat jujenum following Escherichia coli heat table toxin induced fluid accumulation. Pharmacol. Toxicol., 86: 78-82.

19:  Rabbani, G.H., M.J. Albert, H. Rahman and A.K. Chowdhury, 1999. Short chain fatty acids inhibit fluid and electrolyte loss induced by cholera toxin in proximal colon of rabbit in vivo. Dig. Dis. Sci., 44: 1547-1553.

20:  Garino, A., R.B. Canani, M. Iafusco, A. Casola, R. Russo and A. Rubino, 1995. In vivo and in vitro effects of human growth hormone on rat intestinal ion transport. Pediatr. Res., 37: 576-580.
Direct Link  |  

21:  Canani, R.B., M. Iafusco, R. Russo, M. Bisceglia, G. Polito and A. Guarino, 1996. Comparative effects of growth hormone on water and ion transport in rat jejunum ileum and colon. Dig. Dis. Sci., 41: 1076-1081.

22:  Canani, R.B., M. Bisceglia, E. Bruzzese, G. Mallardo and A. Guarino, 1999. Growth hormone stimulates through tyrosine kinase ion transport and proliferation in human intestinal cells. J. Pediatr. Gastroenterol. Nutr., 28: 315-320.
Direct Link  |  

23:  Bosc, L.V.G., N.A. Vidal, R. Prieto and J.A. Tur, 1998. Effect of atrial natriuretic on alpha methyl D glucoside intestinal active uptake in rats. Peptides, 19: 1249-1253.

24:  Pansu, D., A. Bosshard, M.A. Dechelette and M. Vagne, 1980. Effect of pentagastrin secretin and cholecystokinin on intestinal water and sodium absorption in the rat. Digestion, 20: 201-206.

25:  Wang, K.S., T. Ma, F. Filiz, A.S. Verkman and J.A. Bastidas, 2000. Colon water transport in transgenic mice lacking aquaporine 4 water channels. Am. J. Physiol. Gastro Intest. Liver Physiol., 279: 463-470.
Direct Link  |  

26:  Lorca, R.R., M.L. Vizuete, J.L. Venero, Mrevuelta, J. Cano, A.A. Ilundain and M. Echevarria, 1999. Localization of aquaporin 3 mRNA and protein along the gastrointestinal tract of wistar rats. Pflugers Arch., 438: 94-100.

27:  Kozlova, I., A. Dragomir, V. Vanthanouvong and G.M. Roomans, 2000. Effects of nicotine on intestinal epithelial cells in vivo and in vitro a X ray microanalytical study. J. Submicrosc. Cytol. Pathol., 32: 97-102.
Direct Link  |  

28:  Carrithers, S.L., M.J. Hill, B.R. Johnson, S.M.O. Hara and R.N. Greenberg et al., 1999. Renal effect of uroguanylin and guanylin in vivo. Braz. J. Med. Biol. Res., 32: 1337-1344.
CrossRef  |  

29:  Forte, L.R., R.H. Freeman, W.J. Krause and R.M. London, 1999. Guanylin peptides cyclic GMP signaling mechanisms. Braz. J. Med. Biol. Res., 32: 1329-1336.
CrossRef  |  

30:  Date, Y., M. Nakazato and H. Matsuo, 1998. Guanylin family new intestinal peptides regulating salt and water homeostasis. Nippon Rinsho, 56: 2427-2432.

31:  Binder, H.J., P. Sangau and V.M. Rajendrau, 1999. Physiological and molecular studies of clonic H+K+ ATPase. Semin Nephrol., 19: 405-414.

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