Subscribe Now Subscribe Today
Research Article

Formation of Catechin in Callus Cultures and Micropropagation of Rheum ribes L.

M. Farzami Sepehr and M. Ghorbanli
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

The aim of this project was to study of the effects of different nutritional factors on the production of catechin in Rheum ribes L. and micropropagation of this species in agar-solidified medium. Different parts of Rheum ribes sterile seedlings were cultured on MS medium that supplemented with IBA (1 mg L-1) and BA (1 mg L-1) for generation of callus. The concentration of sucrose, vitamins and myo-inositol and ratio of NO3- to NH4+ in the medium was changed and content of catechin was determined by HPLC.Myo-inositol 100 mg L-1 in the medium that supplemented with 0.5 mg L-1 IBA and 0.5 mg L-1 BAP. Rooting medium was ½ MS salts + 0.5 mg L-1 IBA. Regenerated plants were transferred to clay pots a mixture of soil, vermiculite and sand and maintained under 50-70% plants for the first 7 days.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

M. Farzami Sepehr and M. Ghorbanli , 2005. Formation of Catechin in Callus Cultures and Micropropagation of Rheum ribes L. . Pakistan Journal of Biological Sciences, 8: 1346-1350.

DOI: 10.3923/pjbs.2005.1346.1350


1:  Menon, L.G., R. Kuttan and G. Kuttan, 1999. Anti metastasis activity of curumin and catechin. Cancer Lett., 141: 159-165.

2:  Meyer, A.S., M. Heinonen and E.N. Frankel, 1998. Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin and ellagic acid on human LDL oxidation. Food Chem., 61: 71-75.
CrossRef  |  Direct Link  |  

3:  Nanjo, F., K. Goto, R. Seto, M. Suzuki, M. Sakai and Y. Hara, 1996. Scavenging effects of tea catechin and their derivatives on 1 1 diphenyl 2 picrylhydrazyl radical. Free Radicals Biol. Medicine, 21: 895-902.

4:  Waterhouse, A.L., S.F. Price and J.D.M. Cord, 1998. Reversed phase high performance liquid chromatography methods for analysis of win polyphenols. Methods Enzymol., 299: 113-121.

5:  Donovan, J.L., D.L. Luthria, P. Stremple and A.L. Waterhouse, 1999. Analysis of (+)-catechin,(−)-epicatechin and their 3′-and 4′-O-methylated analogs: A comparison of sensitive methods. J. Chromatogr. B: Biomed. Sci. Applic., 726: 277-283.
CrossRef  |  PubMed  |  Direct Link  |  

6:  Khokhar, S., D. Venema, P.C.H. Hollman, M. Dekker and W. Jongen, 1997. A RP HPLC method for determination of tea catechins. Cancer Lett., 114: 171-172.

7:  Collin, H.A. and S. Edwards, 1998. Plant Cell Culture. 1st Edn., BIOS Scientific Publisher Limited, London, New York, pp: 10-15.

8:  Trotin, F., Y. Moumou and J. Vasseur, 1993. Flavanol production of Fagopyrum esculentum hairy and normal roots culture. Phytochemistry, 32: 929-931.

9:  Ono, K., M. Nakao, M. Toyota, Y. Terashi, M. Yamada, T. Kohno and Y. Asakawa, 1998. Catechin production in cultured Polygonum hydropiper cells. Phytochemistry, 49: 1935-1939.

10:  Shibata, S., 1993. Rheum species (Rhubarb) In vitro cultures and production of seeonides. Biotechnol. Agric. For., 21: 25-30.

11:  Rai, P.P., 1978. The production of anthraquinones in callus cultures of Rheum palmatum. Lioydia, 41: 114-116.

12:  Kurosaki, F., H. Nagase and A. Nishi, 1992. Stimulation of anthraquinones production in rhubarb tissue culture by an ethylene generation reagent. Phytochemistry, 31: 2735-2738.

13:  Lal, N. and P.S. Ahuja, 1989. Propagation of Indian rhubarb (Rheum emodi wall) using shoot tip and leaf explants culture. Plant Cell Rep., 8: 493-496.
CrossRef  |  

14:  Lal, N. and P.S. Ahuja, 1993. Assessment of liquid culture procedures for In vitro propagation of Rheum emodi. Plant Cell, Tissue Organ Culture, 34: 223-226.
Direct Link  |  

15:  Lassus, C. and I. Voipio, 1994. Micropropagation of rhubarb with special reference to weaning stage and subsequent growth. Agric. Sci. Finland, 3: 189-194.

16:  Farzami Sepehr, M. and M. Ghorbanli, 2002. Effects of nutritional factors on the formation of anthraquinones in callus cultures of Rheum ribes. Plant Cell Tissue Organ Culture, 68: 171-175.

17:  Kashiwada, Y., G. Nonaka, I. Nishioka and T. Yamagishi, 1988. Galloyl and hydoxycinnamoylglucose from rhubarb. Photochemistry, 27: 1473-1477.

18:  Shokravi, A. and K.A. Nasiri, 1997. Synthesis of 1 2 3 4 5 6 7 8 Octahydro 9 ethoxy 10 hydroxy 1 anthracenone(OEHA). Iran J. Chem. Eng., 16: 10-15.

19:  Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Planta., 15: 473-497.
CrossRef  |  Direct Link  |  

20:  Asher, C.J. and D.G. Edwards, 1983. Modern Solution Culture Techniques. In: Inorganic Plant Nutrition (Encyclopedia of Plant Physiology) New Series, Lauchli, A. and R.L. Bieleski (Eds.). Vol. 153, Apringer, Berlin, pp: 94-119.

21:  Bilia, A.R., I. Morelli, M. Hamburger and K. Hostettmann, 1996. Flavones and a type proanthocyanidins from Prunus prostrata. Phytochemistry, 43: 887-892.

22:  Zenk, M.H., H.E. Shagi and U. Schulte, 1975. Anthraquinones production by suspension cultures of Morinad citrifolia. Planta Medica., Suppl, pp: 79-101.

23:  Yoshida, Y., M. Kiso and T. Goto, 1999. Efficiency of the extraction of catechins from green tea. Food Chem., 67: 429-433.
CrossRef  |  Direct Link  |  

24:  Hagendoorm, M.J.M., L.H.W.V. Plas and G.L. Segers, 1994. Accumulation of anthraquinones in Morinda citrifolia cell suspensions a model system for the study of the interaction between secondary and primary metabolites. Plant Cell Tissue Organ Culture, 38: 227-237.
CrossRef  |  

25:  Donnenburg, H. and D. Knorr, 1996. Generation of colors and flavors in plant cell and tissue cultures. Critical Rev. Plant Sci., 15: 141-168.
Direct Link  |  

26:  Suzuki, H., T. Matsumoto and Y. Mikami, 1984. Effects of nutritional factors on the formation of anthraquinones by Rubia cordifolia cells in suspension culture. Agric. Biol. Chem., 48: 603-610.

27:  Razdan, M.K., 2003. Introduction to Plant Tissue Culture. Science Publishing, INC., Enfield, NH, USA.

28:  Ghorbanli, M. and M.F. Sepehr, 2001. Effects of different plant growth regulators on In vitro regeneration of Prosopis koelziana Burkart. Pajouhesh Sazandegi, 51: 82-87.

29:  Skoog, F. and W. Miller, 1975. The biological action of growth substance. Proceedings of the Symposium of the Society for Experimental Biology, 1975, Cambridge University Press, Cambridge, pp: 118-131.

©  2021 Science Alert. All Rights Reserved