ABSTRACT
The aim of this project was to study of the effects of different nutritional factors on the production of catechin in Rheum ribes L. and micropropagation of this species in agar-solidified medium. Different parts of Rheum ribes sterile seedlings were cultured on MS medium that supplemented with IBA (1 mg L-1) and BA (1 mg L-1) for generation of callus. The concentration of sucrose, vitamins and myo-inositol and ratio of NO3- to NH4+ in the medium was changed and content of catechin was determined by HPLC.Myo-inositol 100 mg L-1 in the medium that supplemented with 0.5 mg L-1 IBA and 0.5 mg L-1 BAP. Rooting medium was ½ MS salts + 0.5 mg L-1 IBA. Regenerated plants were transferred to clay pots a mixture of soil, vermiculite and sand and maintained under 50-70% plants for the first 7 days.
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How to cite this article
M. Farzami Sepehr and M. Ghorbanli, 2005. Formation of Catechin in Callus Cultures and Micropropagation of Rheum ribes
L. . Pakistan Journal of Biological Sciences, 8: 1346-1350.
DOI: 10.3923/pjbs.2005.1346.1350
URL: https://scialert.net/abstract/?doi=pjbs.2005.1346.1350
DOI: 10.3923/pjbs.2005.1346.1350
URL: https://scialert.net/abstract/?doi=pjbs.2005.1346.1350
REFERENCES
- Meyer, A.S., M. Heinonen and E.N. Frankel, 1998. Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin and ellagic acid on human LDL oxidation. Food Chem., 61: 71-75.
CrossRefDirect Link - Donovan, J.L., D.L. Luthria, P. Stremple and A.L. Waterhouse, 1999. Analysis of (+)-catechin,(−)-epicatechin and their 3′-and 4′-O-methylated analogs: A comparison of sensitive methods. J. Chromatogr. B: Biomed. Sci. Applic., 726: 277-283.
CrossRefPubMedDirect Link - Lal, N. and P.S. Ahuja, 1989. Propagation of Indian rhubarb (Rheum emodi wall) using shoot tip and leaf explants culture. Plant Cell Rep., 8: 493-496.
CrossRef - Lal, N. and P.S. Ahuja, 1993. Assessment of liquid culture procedures for In vitro propagation of Rheum emodi. Plant Cell, Tissue Organ Culture, 34: 223-226.
Direct Link - Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRefDirect Link - Yoshida, Y., M. Kiso and T. Goto, 1999. Efficiency of the extraction of catechins from green tea. Food Chem., 67: 429-433.
CrossRefDirect Link - Hagendoorm, M.J.M., L.H.W.V. Plas and G.L. Segers, 1994. Accumulation of anthraquinones in Morinda citrifolia cell suspensions a model system for the study of the interaction between secondary and primary metabolites. Plant Cell Tissue Organ Culture, 38: 227-237.
CrossRef - Donnenburg, H. and D. Knorr, 1996. Generation of colors and flavors in plant cell and tissue cultures. Critical Rev. Plant Sci., 15: 141-168.
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