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Research Article

In vitro Shoot Multiplication and Rooting of a Dessert Banana (Musa sp cv. Anupom)

M.Z. Rahman , M.H. Rahman , M.U. Mullah , N. Nahar , R.S. Sultana , M.A. Bari and M. Hossain
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The effects of various cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (CW) alone or in combinations on shoot multiplication of banana cv. Anupom were investigated. The rate of multiplication varied in different treatments. Among the treatments, MS medium supplemented with 5.0 mg L-1 each of BAP + KIN and 13% coconut water produced the highest number of shoot per explant (5.8±0.154). The number of shoots responded for rooting and their survivability were found higher with IBA than NAA and IAA. MS medium supplemented with 1.0 mg L-1 IBA produced the highest number of roots per shoot (7.±0.245). Plantlets grown without any auxin in the medium gave the least number of roots/shoot (2.60±0.219). The rooted plantlets were successfully transferred in the polybags and finally were well established in the field.

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  How to cite this article:

M.Z. Rahman , M.H. Rahman , M.U. Mullah , N. Nahar , R.S. Sultana , M.A. Bari and M. Hossain , 2005.

In vitro Shoot Multiplication and Rooting of a Dessert Banana (Musa sp cv. Anupom). Pakistan Journal of Biological Sciences, 8: 1298-1302.

DOI: 10.3923/pjbs.2005.1298.1302



1:  Anonymous, 2001. Statistical Yearbook of Bangladesh. Bangladesh Bureau of Statistics. Planning Division, Ministry of Planning, Government of the People's Republic of Bangladesh, Dhaka, Bangladesh, pp: 142

2:  Simmonds, N.W., 1966. Bananas. 2nd Edn., Longman, London, pp: 512

3:  Fitchet, R. and W. De-Winnaar, 1988. Effect of sterilants and nutrient media on the establishment of shoot tips of two banana cultivars in culture. Subtropica (ZAF), 9: 12-16.

4:  Huang, L.C. and K.L. Chi, 1988. Pivotal roles of picloram and gelrite in banana callus cultures. Environ. Exp. Bot., 28: 249-258.

5:  Krikorian, A.A. and S.S. Cronauer, 1984. Aseptic culture techniques for banana and plantain improvement. Econ. Bot., 38: 322-331.
CrossRef  |  Direct Link  |  

6:  Wong, W.C., 1986. In vitro propagation of banana (Musa sp.): Iitiation, proliferation and development of shoot-tip cultures on defined media. Plant Cell Tissue Organ Cult., 6: 159-166.
Direct Link  |  

7:  Novak, F.J., R. Afja, T. Phadvibulya, T. Hermelin and H. Brunner, 1986. Micropropagation and Rediation Sensitivity in Shoot Tip Cultures of Banana and Plantains: Nuclear Techniques and in vitro Culture for Plant Improvement. International Atomic Energy Agency, Vienna, pp: 167-174

8:  Banerjee, N. and E. De Langhe, 1985. A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (banana and plantain). Plant Cell Rep., 4: 351-354.
Direct Link  |  

9:  Eckstein, K. and J.C. Robinson, 1995. Physiological responses of banana (Musa AAA: Cavendish sub-group) in the sub-tropics, iv: Comparison between tissue culture and conventional plant material during the first months of development. J. Hortic. Sci., 70: 549-559.

10:  Vuylsteke, D. and E.A.L. de Langhe, 1985. Feasibility of in vitro propagation of bananas and plantains. Trop. Agric., 62: 323-328.
Direct Link  |  

11:  Razdan, M.K., 1999. An Introduction to Plant Tissue Culture. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, pp: 282

12:  Drew, R.A. and M.K. Smith, 1990. Field evaluation of tissue cultured bananas in South-estern Queensland. Aust. J. Exp. Agric., 30: 569-574.

13:  Vuylsteke, D.R. and R. Ortiz, 1996. Field performance of conventional vs. in vitro propagules of plantain (Musa spp., AAB group). Hortic. Sci., 31: 862-865.
Direct Link  |  

14:  Pradeep, K.P., G. Zacharia, S. Estelitta and A. Suma, 1992. Field performances of banana tissue culture of variety Nendran (Musa AAB). South Ind. Hortic., 40: 1-4.

15:  Faisal, S.M., M.A. Haque and A. Quasem, 1998. Field performance of in vitro plantlets against normal suckers of banana (Musa sapientum) cv. Champa. Plant Tissue Cult., 8: 125-129.

16:  Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRef  |  Direct Link  |  

17:  Cronauer, S.S. and A.D. Krokorian, 1984. Rapid multiplication of banana and plantains by in vitro shoot tip culture. Hortic. Sci., 19: 234-235.
Direct Link  |  

18:  Cronauer, S.S. and A.D. Krikorian, 1984. Multiplication of Musa from excised stem tips. Ann. Bot., 53: 321-328.
Direct Link  |  

19:  Damasco, O.P. and R.C. Barba, 1985. In vitro culture of Saba banana (Musa balbiciana cv. Saba, BBB). Proceedings of the Biotechnology in International Agricultural Research Centers (IARCs) and Bio-technology, Apr. 23-27, International Rice Research Insitute, Los Banos, Philippines, pp: 40-44

20:  Pandey, R.M., R. Doreswamy and L. Sahijram, 1993. . Tissue Culture Propagation of Banana. Technical Bulletin No. 11. Publication Pandey R.M. IIHR, Hesserghatta, Bangalore.

21:  Khatri, A., I.A. Khan, S.H. Siddiqui, M. Ahmed and K.A. Siddiqui, 1998. In vitro culture of indigenous and exotic banana clone for maximising multiplication. Pak. J. Bot., 29: 143-150.

22:  Mohammad, A., I. Hussain, H. Rashid and A. Quraishi, 2000. Enhanced in vitro multiplication of banana (Musa sp.). Plant Tissue Cult., 10: 111-117.

23:  Habiba, U., S. Reza, M.L. Sata, M.R. Khan and S. Hadiuzzaman, 2002. Endogenous bacterial contamination during in vitro culture of table banana: Identification and prevention. Plant Tissue Cult., 12: 117-124.
Direct Link  |  

24:  Azad, M.A.K. and M.N. Amin, 2001. Rapid clonal propagation of banana (Musa sp.) using in vitro culture of floral bud apex. Plant Tissue Cult., 11: 1-9.

25:  De Langhe, F.A.L., 1985. Tissue culture technique for rapid clonal propagation and storage conditions of Musa (Banana and plantain). Plant Cell Rep., 4: 355-359.

26:  Akber, M., M.Z. Hossain and K.S. Roy, 2003. High frequency plant regeneration of banana (Musa sapientum L.) cv. Sagar through in vitro culture. Bangladesh J. Life Sci., 15: 33-38.

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