Subscribe Now Subscribe Today
Research Article

Aphid Transmission of Two Important Potato Viruses, PVY and PLRV by Myzus persicae (Sulz.) and Aphis gossypii (Glov.) in Hatay Province of Turkey

Erdal Sertkaya and Gulsen Sertkaya
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Transmission assays by using aphid vectors were established to determine the effects of two aphid species (Myzus persicae Sulz. and Aphis gossypii Glov. on four solanacaeous test plant species (Capsicum annum L., Lycopersicon esculentum L., Physalis floridana Rydb. and Solanum tuberosum L.). Transmission rates of PVY and PLRV from potato to other test plants were investigated by DAS-ELISA assays. All experiments were carried out at 26:22±2°C (day:night) under a 16:8 h (light:dark) photoperiod conditions. Late 4th instar or early adult M. persicae and A. gossypii apterae were used in the transmission experiments. The aphids were not subjected to preacquisition starving in the assays. Each aphid species were reared on PVY-infected source potato plants for 5 h as Acquisition Access Period (AAP). Then, aphid group including 10 individuals were taken from donor plants were transplanted on to healthy receptor test plants for 15 h as Inoculation Period (IP). For PLRV transmissions, two aphid species reared on source potato plants for 24 h as AAP were transferred on to each healthy test plants as a group including 10 individuals for 24 h as IP. After IP, the aphids were removed from plants mechanically. PVY and PLRV were transmitted in higher rates by M. persicae. PVY and PLRV were more efficiently transmitted from potato to pepper seedlings among the other inoculated solanaceous test plants by both aphid species. The lowest transmission was occurred in tomato (2/15) and P. floridana (3/15) by using A. gossypii. Seed transmission of viruses were not determined by DAS-ELISA in seedlings re-obtained from experimentally infected test plants.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Erdal Sertkaya and Gulsen Sertkaya , 2005. Aphid Transmission of Two Important Potato Viruses, PVY and PLRV by Myzus persicae (Sulz.) and Aphis gossypii (Glov.) in Hatay Province of Turkey. Pakistan Journal of Biological Sciences, 8: 1242-1246.

DOI: 10.3923/pjbs.2005.1242.1246


1:  Salazar, L.F., 1996. Potato Viruses and their Control. International Potato Center, Peru, Pages: 214.

2:  Bunt, A.A., 2001. The Main Viruses Infecting Potato Crops. In: Viruses and Virus-Like Diseases of Potatoes and Production of Seed Potatoes, Lobenstein, G., P.H. Berger, A.A. Brunt and R.H. Lawson (Eds.). Kluwer Academic Publishers, Dordrecht, pp: 65-67.

3:  Van Regenmortel, M.H.V., C.M. Fauquet and D.H.L. Bishop, 2000. Virus Taxanomy: The Classification and Nomenculature of Viruses. Academic Press, New York, pp: 1167.

4:  Eastop, V.F., 1977. Worldwide Importance of Aphids Virus Vectors. In: Aphids as Virus Vectors, Haris, K.F. and K. Maramorosch (Eds.). Academic Press, New York, pp: 3-61.

5:  Khush, G.S. and D.S. Brar, 1991. Genetics of resistance to insects in crop plants. Adv. Agron., 45: 223-274.

6:  Leclant, F. and J.P. Deguine, 1994. Aphids (Hemiptera: Aphididae). In: Insect Pests of Cotton, Mathews, G. and J. Tunstall (Eds.). CAB International, Oxon (GBR), pp: 285-323.

7:  Nie, X. and R.P. Singh, 2001. A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves and tubers. J. Virol. Methods, 91: 37-49.
Direct Link  |  

8:  Lawson, C., W. Kaniewski, L. Haley, R. Rozman, C. Newell, P. Sanders and N.E. Tumer, 1990. Engineering resistance to mixed virus infection in a commercial potato cultivar: Resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/Technology, 8: 127-134.
CrossRef  |  Direct Link  |  

9:  Atreya, C.D., P.L. Atreya D.W. Thornbury and T.P. Pirone, 1992. Site-directed mutations in the potyvirus HC-PRO gene affect helper component activity, virus accumulation and symptom expression in infected tobacco plants. Virology, 191: 106-111.
Direct Link  |  

10:  Pirone, T. and S. Blanc, 1996. Helper dependent vector transmission of plant viruses. Ann. Rev. Phytopathol., 34: 227-247.
CrossRef  |  Direct Link  |  

11:  Blanc, S., E.D. Ammar, S. Garcia-Lampasona, V.V. Dolja, C. Llave, J. Baker and T.P. Pirone, 1998. Mutations in the potyvirus helper component protein: Effects on interactions with virions and aphid stylets. J. Gen. Virol., 79: 3119-3122.
Direct Link  |  

12:  Yilmaz, M.A., S. Baloglu and Y.Z. Nas, 1990. Cukurova Bolgesinde yeti1tirilen turfanda patateslerde patates yaprak kivirciklik virusunun (PLRV) ELISA testi ile surveyi. C.U. Ziraat Fak. Derg., 3: 95-106.

13:  Cali, S. and N. Yalcin, 1991. Ithal edilmis tohumluk patateslerde onemli virus hastaliklarinin DAS-ELISA ve diger yontemler ile araptirilmasi. Turk. Fitopat. Kong. Bildirileri, Izmir, pp: 333-336.

14:  De Boxy, J.A. and J.P.H. van der Want, 1987. Viruses of Potatoes and Seed-Potato Production. 2nd Edn., Wageningen, Netherlands.

15:  Clark, M.F. and A.N. Adams, 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol., 34: 475-483.
CrossRef  |  PubMed  |  Direct Link  |  

16:  Ragsdale, D.W., E.B. Radcliffe and C.D. Di Fonzo, 2001. Epidemiology and Field Control of PVY and PLRV. In: Virus and Virus-like Diseases of Potatoes and Production of Seed-Potatoes, Loebenstein, G., P. Berger, A.A. Brunt and R. Lawson (Eds.). Kluwer Academic Publishers, Netherlands, pp: 237-270.

17:  Bradley, R.H.E. and D.W. Rideout, 1953. Comparative transmission of potato virus Y by four aphid species that infest potato. Can. J. Zool., 31: 333-341.
CrossRef  |  

18:  Hille, R.L.D., 1972. Aphids: Their Life Cycles and Their Role as Virus Vectors. In: Viruses of Potatoes and Seed Potato Production, Bokx, de J.A. (Ed.). Pudoc Publisher, Wageningen, pp: 36-56.

19:  Robert, Y. and J. Rouze-Jouan, 1971. Remieres observations sur le role de la temperature au moment de la transmission de 2`enroulement par Aulacorthum solani Kltb. Macrosiphum euphorbiae Thomas et Myzus persicae Sulz. Potato Res., 14: 154-157.

20:  Woodford, J.A.T., C.A. Jolly and C.S. Aveyard, 1995. Biological factors influencing the transmission of potato leafroll virus by different aphid species. Potato Res., 38: 133-141.
CrossRef  |  Direct Link  |  

21:  Robert, Y. and D. Bourdin, 2000. Aphid Transmission of Potato Viruses. In: Virus and Virus-Like Diseases of Potatoes and Production of Seed Potatoes, Lobenstein, G., P.H. Berger, A.A. Brunt and R.H. Lawson (Eds.). Kluwer Academic Publishers, Dordrecht, pp: 195-225.

22:  Sasaya, T., L. Torrance, G. Cowan and A. Ziegler, 2000. Aphid transmission studies using helper component proteins of potato virus Y expressed from a vector derived from potato virus X. J. Gen. Virol., 81: 1115-1119.

23:  Romero, A., B. Blanco-Urgoiti, M.J. Soto, A. Fereres and F. Ponz, 2001. Characterization of typical pepper-isolates of PVY reveals multiple pathotypes within a single genetic strain. Virus Res., 79: 71-80.
CrossRef  |  PubMed  |  Direct Link  |  

24:  Fereres, A., P. Perez, C. Gemen and F. Ponz, 1993. Transmission of spanish pepper- and potato-PVY isolates by aphid (Homoptera: Aphididae) vectors: Epidemiological implications. Commun. Ecosystem Ecol., 22: 1260-1265.
Direct Link  |  

25:  Foster, G.N. and J.A.T. Woodford, 1997. Melon and cotton aphid (Aphis gossypii Glover: (Homoptera: Aphididae) as a potantial new virus vector of potatoes in Scotland. Entomologist, 116: 175-178.

26:  Raboudi, F., Ben Moussa, H. Makni, M. Marrakchi and M. Makni, 2002. Serelogical detection of plant viruses in their aphid vectors and host plants in Tunisia. OEPP/Eppo Bull., 32: 495-498.
Direct Link  |  

27:  Lobenstein, G., 1999. Virus diseases of potatoes-diagnosis and control. Proceedings of 1st Israel-Turkish Workshop on Detection of Virus Diseases by Advanced Techniques and Control, Aug. 22-29, Adana, Turkey, pp: 40-45.

28:  Moraes, F.H.R., A.D.R. Figuera and R.D.C. Santos, 1999. Comparative study of some properties of five potato virus Y isolates. Summa Phytopathologica, 25: 117-125.

©  2021 Science Alert. All Rights Reserved