Research Article

Effect of UV-C Radiation on Drought Tolerance of Alfalfa (Medicago sativa) Callus

Ali Akbar Ehsanpour and Roya Razavizadeh
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Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area, consequently, the study of plant responses to water stress is important. In this study UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0,15, 30 and 60 min and then, were transferred to MS medium containing 0, 2, 5, 10, 20 and 30% PEG. All explants were kept in the dark in the culture room. Calluses tolerate to osmotic stress were selected according to fresh weight. Results showed that radiation of UV-C for 60 min increased the osmotic tolerance and decreased the effect of drought stress.

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  How to cite this article:

Ali Akbar Ehsanpour and Roya Razavizadeh , 2005. Effect of UV-C Radiation on Drought Tolerance of Alfalfa (Medicago sativa) Callus. Pakistan Journal of Biological Sciences, 8: 1221-1224.

DOI: 10.3923/pjbs.2005.1221.1224



  1. Arcioni, S., F. Damiani, M. Pezzotti and E. Lupotto, 1990. Alfalfa, Lucerne (Medicago sp.). In: Biotechnology in Agriculture and Forestry, Bajaj, Y.P.S. (Ed.). Spronger, New York, pp: 197-241

  2. Steppler, H.A., 1987. ICRAF: A Decade of Agroforestry Development. In: Agroforestry, a Decade of Development, Steppler, H.A. and P.K.R. Nair (Eds.). International Council for Research on Agroforestry, Nairobi, Kenya, pp: 13-24

  3. Ehsanpour, A.A. and M.G.K. Jones, 2001. Plant regeneration from mesophyll protoplasts of potato (Solanum tuberosome L.) cultivar Delaware using Silver Thiosulfate (STS). J. Sci., 12: 103-110.

  4. Rudulier, D.L., A.R. Strom and A.M. Dandekar, 1984. Molecular biology of osmoregulation. Science, 224: 1064-1068.

  5. Danon, A. and P. Gallois, 1998. UV-C radiation induces apoptotic like changes in Arabidopsis thaliana. FFBs. Lett., 437: 131-136.

  6. Kolb, C.A., M.A. Kaser, J. Kopecky, G. Zota, M. Riedere and E.E. Pfundel, 2001. Effect of natural intensities of visible and ultraviolet radiation on epidermal UV screening and photosynthesis in grape leaves. Plant Physiol., 127: 863-875.
    CrossRef  |  PubMed  |  

  7. Woodall, G.S. and G.R. Stewart, 1998. Do anthocanin play a rol in UV protection of the red juvenile leaves of Syzygium?. J. Exp. Bot., 49: 1447-1450.

  8. Dami, I. and H.G. Hughes, 1997. Effect of PEG induced water stress on in vitro hardening of valiant grape. Plant Cell Tiss. Org. Cult., 47: 97-101.

  9. Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
    CrossRef  |  Direct Link  |  

  10. Girma, F.S. and D.R. Kreig, 1992. Osmotic adjastment in sorghum. Plant Physiol., 99: 577-582.

  11. Julie, E.H., A.E. Richardson and R.J. Simpson, 1999. Phytase and acid phosphatase activities in extracts from roots of temperate pasture grass and legum seedlings. Aust. Plant Physiol., 26: 801-809.

  12. Larkin, P.J. and W.R. Scowcroft, 1981. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor. Applied Genet., 60: 197-214.
    CrossRef  |  Direct Link  |  

  13. Barret-Lennard, E.G., A.D. Robson and H. Greenway, 1982. Effect of phosphorous deficiency and water defction phosphatase activities from wheat leaves. J. Exp. Bot., 33: 682-695.
    CrossRef  |  

  14. Navarro, J.M., M.A. Botella, A. Cerda and V. Martinez, 2001. Phosphorus uptake and translocation in salt-stressed melon plants. J. Plant Physiol., 158: 375-381.
    CrossRef  |  

  15. Zhu, J.K., 2002. Salt and drought stress signal transduction in plants. Annu. Rev. Plant Biol., 53: 247-273.
    CrossRef  |  PubMed  |  Direct Link  |  

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