Subscribe Now Subscribe Today
Research Article
 

Effect of in vitro Glycation of Human Placental Collagen (Type IV) on Platelet Aggregation



Goodarzi Mohammad Taghi , Rezaei Mohsen , Piry Hossein and Amiry Saied
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

The aim of this study was to analyze the in vitro glyation of human placental collagen (type IV) and to test the effect of glycation on its platelet aggregation activity. After isolation of collagen IV from a normal human placenta, it was glycated using different glucose concentration and different time of incubation. Glycation was measured using thiobarbituric acid colorimetric reaction. Using a turbidimetric method effect of glycated collagen on platelet aggregation was studied and compared with those of non-glycated collagen. The data obtained from this study indicated that collagen underwent in vitro glycation reaction and the level of glycation was dependent on glucose concentration and time of treatment with glucose (p<0.01). Glycated collagen increased platelet aggregation compared with native collagen (p<0.01). These results suggest that hyper-aggregability of platelet in interaction with glycated collagen can be the result of modification in collagen structure. The observed carbohydrate-induced modification apparently have a major impact on molecular conformation of the collagen IV, which might be the molecular basis for pathophysiological changes observed in diabetic microvascular complications.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Goodarzi Mohammad Taghi , Rezaei Mohsen , Piry Hossein and Amiry Saied , 2005. Effect of in vitro Glycation of Human Placental Collagen (Type IV) on Platelet Aggregation. Pakistan Journal of Biological Sciences, 8: 1203-1206.

DOI: 10.3923/pjbs.2005.1203.1206

URL: https://scialert.net/abstract/?doi=pjbs.2005.1203.1206

REFERENCES
1:  Voziyyan, P.A., R.G. Khalifah, C. Thibaudeau, A. Yildiz, J. Jacob, A.S. Serinai and B.G. Hudson, 2003. Modification of proteins in vitro by physiological levels of glucose: Pyridoxamine inhibits conversion of Amadori intermedate to advanced glycation end products through binding of redox metal ions. J. Biol. Chem., 278: 46616-46624.
Direct Link  |  

2:  Thornalley, P.J., 2003. Use of aminoguanidine (Pimagedine) to prevent the formation of advanced glycation endproducts. Arch. Biochem. Biophys., 419: 31-40.

3:  Forbes, J.M., M.E. Cooper, M.D. Oldfield and M.C. Thomas, 2003. Role of advanced glycation end products in diabetic nephropathy. J. Am. Soc. Nephrol., 14: 254-258.
Direct Link  |  

4:  Beisswenger, P.J., B.S. Szwergold and K.T. Yeo, 2001. Glycated proteins in diabaetes. Clin. Lab. Med., 21: 53-78.

5:  Rittie, L., A. Berton, J.C. Monboisse, W. Horebeck and P. Gilery, 1999. Decreased concentration of glycated collagen lattices with impaired matrix metalloproteinase production. Biochem. Biophys. Res. Commun., 264: 488-492.

6:  Raabe, H.M., H. Molsen, S.M. Mlinaric, Y. Acil and G.H.G. Sinnecker et al., 1996. Biochemical alterations in collagen IV induced by in vitro glycation. Biochem. J., 319: 699-704.
Direct Link  |  

7:  Tsilibary, E.C., A.S. Charison, L.A. Reger, R.M. Wohlhueter and L.T. Fucht, 1988. The effect of nonenzymatic glucosylation on the binding of the main noncollagenous NC1 domain to type IV collagen. J. Biol. Chem., 263: 4302-4308.
Direct Link  |  

8:  Tarsio, J.F., L.A. Reger and L.T. Furcht, 1987. Decresed interaction of fibronectin, type IV collagen and heparin due to nonenzymatic glycation: Implications for diabetes mellitus. Biochemistry, 26: 1014-1020.
CrossRef  |  

9:  Le Pape, A., N. Gutman, J.D. Guitton, Y. Legrand and J.P. Muh, 1983. Non enzymatic glycosylation increase platelet aggregation potency of collagen from placenta of diabetic human beings. Biochem. Biophys. Res. Commun., 111: 602-610.
PubMed  |  Direct Link  |  

10:  Brwonlee, M., S. Pongor and A. Cerami, 1983. Covalent attachment of soluble proteins by nonenzymatically glycosylated collagen. J. Exp. Med., 158: 1739-1744.
Direct Link  |  

11:  Yamagish, S., D. Edelstein, X. Du and M. Brownlee, 2001. Hyperglycemia potentiates collagen-induced platelet activation through mitochondrial superoxide overproduction. Diabetes, 50: 1491-1494.
PubMed  |  

12:  Vinik, A.I., T. Erbas, T.S. Park, N. Roger and G.L. Pittenger, 2001. Platelet dysfunction in type 2 diabetes. Diabetes Care, 24: 1476-1485.
CrossRef  |  PubMed  |  Direct Link  |  

13:  Glanville, R., 1979. Isolation and charaterization of a native placental basement membrane collagen. Eur. J. Biochem., 95: 383-389.
PubMed  |  

14:  Raabe, H.M., H. Molsen, S.M. Mlinaric, Y. Acil and G.H.G. Sinnecker et al., 1996. Biochemical alterations in collagen IV induced by in vitro glycation. Biochem. J., 319: 699-704.
Direct Link  |  

15:  Walker, J.M., 1996. SDS Polyacrylamide Gel Electrophoresis of Proteins. In: The Protein Protocols Handbook, Walker, J.M. (Ed.). Humana Press, New Jersey, pp: 55-61.

©  2021 Science Alert. All Rights Reserved