Subscribe Now Subscribe Today
Fulltext PDF
Research Article

Effects of Oral Administration of Water Extract of Nigella sativa on Serum Concentrations of Insulin and Testosterone in Alloxan-induced Diabetic Rats

Kamal Mohmoud Saleh Mansi

The present study was designed to evaluate the role of a medicinal plant for management of diabetes instead of manufactured drugs that led to much complication. Water extract of Nigella sativa was investigated for hypoglycemic effect in diabetic rats in addition to some hormones related to diabetes mellitus. The extract induced significant reduction in serum glucose from (7.83±1.25) to (6.7±1.10), serum insulin increased from (0.55±0.08) to (0.65±0.06) and testosterone concentration significantly increased in treated diabetic animals (7.58±0.21) compared to diabetic animals (6.63±0.58). The results suggest the beneficial role of N. sativa as hypoglycemic agents and as a protective effect against pancreatic β-cells damage from alloxan induced diabetes in rats by decreasing oxidative stress and preserving pancreatic β-cells integrity and also suggest that the anti diabetic effect of N. sativa may be attributed to increased glucose metabolism. This in turn produces increase level of insulin in serum and testosterone concentration. Which might be related with the functional activity of the reproductive glands and the correlation with the functional state of the pancreatic β-cells.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Kamal Mohmoud Saleh Mansi , 2005. Effects of Oral Administration of Water Extract of Nigella sativa on Serum Concentrations of Insulin and Testosterone in Alloxan-induced Diabetic Rats. Pakistan Journal of Biological Sciences, 8: 1152-1156.

DOI: 10.3923/pjbs.2005.1152.1156


Abdel, M.A., M. El-Feki and E. Saleh, 1998. Effect of N. sativa, fish oil and localized on alloxan diabetic rats. Biochemical and histopath studies. J. Egypt. Ger. Soc. Zool., 23: 237-265.

Agel, M. and R. Shaheen, 1996. Effects of the volatile oil of Nigella sativa seeds on the uterine smooth muscle of rat and guinea pig. J. Ethnopharmacol., 52: 23-26.
CrossRef  |  Direct Link  |  

Ahmad, F., P. Khalid, M.M. Khan, M. Chaubey, A.K. Rastogi and J.R. Kidwai, 1991. Hypoglycemic activity of pterocarpus marsupium wood. J. Ethenopharmacol., 35: 71-75.
Direct Link  |  

Al-Awadi, F.M. and K.A. Gumaa, 1987. Studies on the activity of individual plants of an antidiabetic plant mixture. Acta Diabetol., 24: 37-41.
CrossRef  |  Direct Link  |  

American Diabetes Association (ADA), 1997. Clinical practice recommendation. Diabetes Care, 20: 1-70.

Barrett-Corner, E., 1992. Lower endogenous androgen levels and dyslipidimia in men with non-insulin dependent diabetes mellitus. Ann. Int. Med., 117: 897-911.

Barrett-Corner, E., K. Khaw and S.S.C. Yen, 1990. Endogenous sex hormone levels in order adult men with diabetes mellitus. Am. J. Epidemiol., 132: 895-901.
Direct Link  |  

El-Tahir, K.E.H., M.M.S. Ashour and M.M. Al-Harbi, 1993. The cardiovascular actions of the volatile oil of the black seed in rats. Elucidation of the mechanism(s). General Pharmacol., 24: 1123-1131.
PubMed  |  

Foster, D.W., 1994. Diabetes Mellitus Harrison`s Principles of Internal Edicine. McGraw Hill, New York, USA., pp: 1979-1981.

Haffner, S.M., J. Shaten, M.P. Stern, G.D. Smith and L. Kuller, 1996. Low levels of sex hormone-binding globulin and testosterone predict the development of non-insulin-dependent diabetes mellitus in men. Am. J. Epidemiol., 143: 889-897.
Direct Link  |  

Hanafy, M.S.M. and M.E. Hatem, 1991. Studies on the antimicrobial activity of Nigella sativa seed (black cumin). J. Ethnopharmacol., 34: 275-278.
CrossRef  |  Direct Link  |  

Hawsawi, Z.A., B.A. Ali and A.O. Bamosa, 2001. Effect of N. sativa (Black seed) and thymoquin on blood glucose in albino rats. Ann. Saudi Med., 21: 242-244.
Direct Link  |  

Holman, R.R. and R.C. Turne, 1991. Oral Agents and Insulin in the Treatment of NIDDM Textbook of Diabetes. Blackwell, Oxford, pp: 467-469.

Ismail, M., Y. Ozbek and R. Ustun, 2003. Effects of Nigella sativa on serum concentration TSH and glucose in induced diabetic rabbits. J. Irish Vet., 56: 446-484.

Khosia, P., D. Gupts and R.K. Nagpal, 1995. Effect of Trigonella foenom graecum (fenugreet) on blood glucose in normal and diabetic rats. Indian J. Physiol. Pharmacol., 39: 173-174.

Manisckam, M., M. Ramanthan, M.A. Jahromi, J.P. Chasouria and A.B. Ray, 1997. Antihyperglycemic activity of phenolics from Pterocapus marsupium. J. Nature Products, 60: 609-610.
PubMed  |  

Ozturk, Y., V.M. Altan and N. Ari, 1998. Diabetic complication in experimental models. Turk. J. Med. Sci., 22: 331-341.
Direct Link  |  

Palmieri, V., J.N. Bella, D.K. Arnett, J.E. Liu and A. Oberman et al., 2001. Effect of type 2 diabetes mellitus on left ventricular geometry and systolic function in hypertensive subjects: Hypertension genetic epidemiology network (HyperGEN) study. Circulation, 103: 102-107.
CrossRef  |  Direct Link  |  

Rai, V., U. Lyer and U.V. Mani, 1997. Effect of Tulasi (Ocimum sanctum) leaf powder supplementation on blood sugar levels serum lipids and tissue lipids in diabetic rats. Plant Foods Hum. Nutr., 50: 9-16.
PubMed  |  Direct Link  |  

Rao, B.K., R. Giri, M.M. Kesavuln and C.H. Apparao, 1997. Herbal medicine in the management of diabetes mellitus. Manphar Vaidhya Patrikal, 1: 33-35.
Direct Link  |  

Saks, D.B., 1997. Implication of the revised criteria for diagnosis and classification of diabetes mellitus. Clin. Chem., 43: 2230-2232.
Direct Link  |  

Tuk, J., 2003. Effect of N. sativa on liver necrosis. Vet. Anim. Sci., 27: 141-152.

Valiathan, M.S., 1998. Healing plants. Curr. Sci., 75: 1122-1127.
Direct Link  |  

Yenigumm, M., 1997. Cardiovascular Diabetes. Istanbul University Printing House, Istanbul, Turkey, pp: 2230-2233.

Zaoui, A., Y. Cherrah, M.A. Lacaille-Dubois, A. Settaf, H. Amarouch and M. Hassar, 2000. Diuretic and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat. Therapie, 55: 379-382, (In French).
PubMed  |  Direct Link  |  

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract