Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Research Article
 

Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Anther Culture of Rice



Md. Monirul Islam, Sanjay Kumar Adhikary , Purnendu Gain , Md. Mizanur Rahman and Noor-e-Alam Siddique
 
ABSTRACT

An experiment was conducted to find the effects of different concentrations and combinations of growth regulators viz. 2, 4 D, IAA, α-NAA and Kinetin on callus induction from the anthers of a commercial hybrid rice line IR-69690, developed by Bangladesh Rice Research Institute and subsequent plant regeneration. N6 medium was used as basal medium. Callus induction frequencies in different media combinations ranged from 1.2 to 35.5%. The medium supplemented with 2, 4 D 1 mg L-1, α-NAA 2 mg L-1 and Kinetin 1 mg L-1 was found most effective for callus induction (35.5%). Regeneration of plants from the callus on agarified MS medium supplemented with α-NAA 0.5 mg L-1 and Kinetin 3 mg L-1 was also variable and ranged from 16.7 to 69.3%. Calli derived from the media supplemented with 2, 4 D 1 mg L-1, α-NAA 2 mg L-1 and Kinetin 1 mg L-1 also showed better performance for plant regeneration (69.3%) and among these plants 56.14% were green. However the callus induction medium containing α-NAA 1 mg L-1 and Kinetin 1 mgL-1 directly produced green regenerated plants in higher frequency (70%), without transferring the calli on to the regeneration medium; but rate of callus induction in this medium was very low (6%).

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Md. Monirul Islam, Sanjay Kumar Adhikary , Purnendu Gain , Md. Mizanur Rahman and Noor-e-Alam Siddique , 2004. Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Anther Culture of Rice. Pakistan Journal of Biological Sciences, 7: 331-334.

DOI: 10.3923/pjbs.2004.331.334

URL: https://scialert.net/abstract/?doi=pjbs.2004.331.334

REFERENCES
Chen, C.C., 1977. Induction of rice plantlets from anther culture. Bot. Bull. Acad. Sinica, 17: 18-24.

Chen, Y., 1988. In vitro Development of Plant from the Microspores of Rice. In: Plant Somatic Genetics and Crop Improvement, Hu, H. and Y. Chen (Eds.). Beijing University Press, Beijing, pp: 27-67.

Genovesi, A.D. and C.W. Magil, 1979. Improvement rate of callus and plant regeneration from rice anther culture following cold shock. Crop Sci., 19: 662-664.

Hu, H., 1988. Use of Haploids in Crop Improvement. IRRI, Los Banos, Philippines, pp: 75-84.

Inoue, M. and E. Maeda, 1980. Absorption and metabolism of radioactive auxins in the induced rice callus. Jap. J. Crop Sci., 50: 318-322.

Isabelle, R. and B.F. Adleson, 1990. Production of double haploid rice plants (Oryza sativa L.) by anther culture. Plant Cell Tiss. Organ Cult., 21: 165-170.
CrossRef  |  

Islam, M.M., G.I. Ivanov and M. Ahmed, 1999. The effect of plant growth regulators on callus induction and regeneration of platelet in anther culture of wheat. Khulna University Studies, 1: 273-276.

Islam, M.M., S.M.K. Shaifulla and G.M. Raihan, 2002. Callus induction and haploid plant regeneration in anther culture of rice. Khulna University Studies.

Maheshwari, S.C., A. Rashid and A.K. Tyagi, 1983. Anther/Pollen culture for production of haploid and their utility. IAPTC Newslett., 41: 2-9.

Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Planta., 15: 473-497.
CrossRef  |  Direct Link  |  

Niizeki, H. and K. Oono, 1968. Influence of genotype and culture medium of microspore callus induction and green plant regeneration in anthers of Oryza sativa. Physiol. Plant, 63: 309-314.

Niizeki, H. and K. Oono, 1968. Induction of haploid rice plant from anther culture. Proc. Jap. Acad., 44: 554-557.
Direct Link  |  

Qu, R.D. and Y. Chen, 1983. A preliminary research on the function of enhancement of callus induction frequency by cold pre-treatment in rice anther culture. Acta Phytophysiol. Sinica, 9: 375-381.

Shen, J.H., M.F. Li, Y.Q. Chcn and Z.H. Zhang, 1982. Breeding by anther culture in rice variety improvement. Sci. Agric. Sinica, 2: 15-19.

Wang, C.C., C.S. Sun and C.C. Chu, 1977. An effect of culture factors in vitro on the production of albino pollen plantlets of rice. Acta Botanica Sinica, 19: 190-198.

Yang, H.Y. and C. Zhou, 1979. Experimental research on the two pathways of pollen development Oryza sativa L. Acta Botanica Sinica, 21: 345-351.

Zapata, F.J., L.B. Torrizo and A. Ando, 1995. Current developments in plant biotechnology for genetic improvement: the case of rice (Oryza sativa L.). World J. Microbiol. Biotechnol., 11: 393-399.
CrossRef  |  Direct Link  |  

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract