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Pakistan Journal of Biological Sciences

Year: 2004 | Volume: 7 | Issue: 12 | Page No.: 2202-2207
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Research Article

Effects of α-Amylase, Protease and Lipase on Haruan (Channa striatus) Mucus Extract Antinociceptive Activity in Mice

Z.A. Zakaria, M.R. Sulaiman, A.M. Mat Jais and M.N. Somchit

ABSTRACT

This study was attempted to preliminarily establish the basic chemical properties of the bioactive compound responsible for Channa striatus (Haruan) mucus extract antinociceptive activity using the acetic acid-induced abdominal constriction test in mice. Pre-treatment of extract with enzymes were carried out at the temperature of 40°C for 60 min. The extract, pre-treated with distilled water (DH2O), was found to show a significant and concentration-dependent antinociceptive activity with the 25 and 50% concentration extracts showing insignificant activity when compared together. The extracts, at the concentrations of 50 and 100%, were pre-treated with 10% concentration α-amylase and lipase and 0.1% concentration protease, respectively. The extracts, pre-treated with α-amylase or protease, were found to produce a concentration-dependent activity, which are, however, not significant when compared to the extracts pre-treated with DH2O (positive control). Interestingly, pre-treatment of the extracts with lipase enhanced the activity significantly (p<0.001). However, this activity did not follow the concentration-dependent pattern. The failure of -amylase and protease to influence the extracts antinociception seems to suggest that the bioactive compound is neither a simple carbohydrate nor protein. Meanwhile, the ability of lipase to enhance the extracts activity, especially of the 50% concentration extract, lead to suggestions that the lipid may act as a carrier of the bioactive compound or the bioactive compound itself is a lipid-based compound. Finally, the bioactive compound is suggested to be a short chain macromolecule, which is resistant to the effect of the three respective enzymes. However, further studies need to be carried out to clarify this finding before the real chemical properties of the bioactive compound can be ruled out.
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