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Research Article
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Isolation and Identification of an Antifungal Sesquiterpene Alcohol from Amboyna Wood |
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Irawan W. Kusuma ,
Tomoko Ogawa ,
Kazutaka Itoh
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Sanro Tachibana
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ABSTRACT
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An antifungal compound was isolated from the n-hexane solubles in methanolic extracts of amboyna wood by bioassay-guided fractionation using Pleurotus pulmonarius, a wood-rotting fungus. The compound was identified as β-eudesmol, a sesquiterpene alcohol with an eudesmane skeleton, based on instrumental analyses (Ultraviolet absorption, Mass spectrometry and 1H and 13C-Nuclear Magnetic Resonance spectroscopy) and a melting point test. The compound showed 21% of the antifungal activity of the methanolic extract as a control. Antifungal assays against P. pulmonarius showed that addition of β-eudesmol at different concentrations affected the inhibition of fungal growth. Furthermore, the activity to inhibit the growth of P. pulmonarius seemed to be dependent on the concentration of β-eudesmol.
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