ABSTRACT
The present study was conducted with an aim of establishing the best organic solvent system that can yielded the Channa striatus extract with highest antinociceptive activity and also to determine the polarity of the respective bioactive compound using the acetic acid- induced abdominal constriction test. Briefly, the Channa striatus fillet were extracted separately with methanol, ethanol, chloroform, chloroform:methanol and distilled water overnight. This procedure is repeated three times and each of the supernatant obtained was pooled together and then subjected to the evaporation process. The extracts obtained was prepared in the doses of 0.5, 1.0, 2.0, 4.0 and 8.0 g kg-1 and administered subcutaneously (SC) into mice. Thirty min after the administration of extracts, 0.6% acetic acid was administered intraperitoneally (IP). All solutions were administered in the volume of 10 mL kg-1. From the data obtained, it can be concluded that the bioactive compounds to be of two types, the polar compound that is water-soluble and the non-polar compound that is fat-soluble. These two compounds might act alone, as in DH2O and chloroform extracts or act together, as in CM, methanol and ethanol extracts. Furthermore, the ability of this compound(s) to exhibit the antinociceptive activity in all the three types of solvents also suggested the presence of peptide compound. However, further investigation need to be carried out in order to isolate and identify the respective compound. In addition, methanol and chloroform:methanol were found to be the best solvents for further isolation of the respective bioactive compound of Channa striatus.
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How to cite this article
Z.A. Zakaria, M.N. Somchit, M.R. Sulaiman and A.M. Mat Jais, 2004. Preliminary Investigation on the Antinociceptive Properties of Haruan (Channa
striatus) Fillet Extracted with Various Solvent Systems. Pakistan Journal of Biological Sciences, 7: 1706-1710.
DOI: 10.3923/pjbs.2004.1706.1710
URL: https://scialert.net/abstract/?doi=pjbs.2004.1706.1710
DOI: 10.3923/pjbs.2004.1706.1710
URL: https://scialert.net/abstract/?doi=pjbs.2004.1706.1710
REFERENCES
- Montecucchi, P.C., R. de Castiglione and V. Erspamer, 1981. Identification of dermorphin and hyp-dermorphin in skin extracts of the brazillian frog Phyllomedusa rhodei. Int. J. Pept. Protein Res., 17: 316-321.
PubMed - Mat Jais, A.M., R. McCullock and K. Croft, 1994. Fatty acid and amino acid composition in Haruan as a potential role in wound healing. Gen. Pharmacol.: Vasc. Syst., 25: 947-950.
CrossRefPubMedDirect Link - Mat Jais, A.M., Y.M. Dambisya and T.L. Lee, 1997. Antinociceptive activity of Channa striatus (Haruan) extracts in mice. J. Ethnopharmacol., 57: 125-130.
CrossRefDirect Link - Dambisya, Y.M., T.L. Lee, V. Sathivulu and A.M. Mat Jais, 1999. The influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus (Haruan) extracts in mice. J. Ethnopharmacol., 66: 181-186.
CrossRefDirect Link - Zimmermann, M., 1983. Ethical guidelines for investigations of experimental pain in conscious animals. Pain, 16: 109-110.
CrossRefPubMedDirect Link - Correa, C.R., D.J. Kyle, S. Chakravarty and J.B. Calixto, 1996. Antinociceptive profile of the pseudopeptide B2 bradykinin receptor antagonist NPC 18688 in mice. Br. J. Pharmacol., 117: 552-558.
PubMedDirect Link - Zakaria, Z.A., M.R. Sulaiman, A.M.M. Jais and M.N. Somchit, 2004. Effects of -amylase, protease and lipase on haruan (Channa striatus) mucus extract antinociceptive activity in mice. Pak. J. Biol. Sci., 7: 2202-2207.
CrossRefDirect Link