Subscribe Now Subscribe Today
Research Article

Immunization of Balb/C Mice by Protein Fragments of Lizard Leishmania promastigote

B. Kazemi , F. Moazzen , A. Abadi , A. Ghadjari , M. Bandehpour and N. Seyed
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

The objective of this study was the immunization of balb/C mice by protein fragments of lizard Leishmania promastigote. Mice were divided in 6 case groups and one as control. Each group received a fraction of lizard Leishmania promastigote. Then active pathogenic Leishmania major challenged them separately. We followed up all the case groups (6 groups) till five months after the challenge with Leishmania major together with control group and recorded the lesions diameter. None of the mice manifested detectable wound or nodules, except those at group 6 but the difference compared to control group was not significant according to the Mann-Whitney analytical test.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

B. Kazemi , F. Moazzen , A. Abadi , A. Ghadjari , M. Bandehpour and N. Seyed , 2004. Immunization of Balb/C Mice by Protein Fragments of Lizard Leishmania promastigote. Pakistan Journal of Biological Sciences, 7: 1699-1702.

DOI: 10.3923/pjbs.2004.1699.1702


1:  Modabber, F., 1995. Leishmaniasis. Tropical disease research. 12th Program Report of UNDP/World Bank/WHO. Special Program for Research and Training in Tropical Disease, pp: 135.146.

2:  WHO, 1997. Leishmaniasis TDR. Progress 13th Program Report, pp: 101-105.

3:  Marsden, P.D., 1984. Selective primary health care: Strategies for control disease in the developing world. XIV. Leishmaniasis. Rev. Infect. Dis., 6: 736-744.

4:  Khalil, E.A., A.M. Elhassan, E.E. Zijlstra, O.F. Osman and I.A. Eljack et al., 2000. Safety and immunogenicity of an autoclaved Leishmania major vaccine. East. Afr. Med. J., 77: 468-470.

5:  Mahmoodi, M., A. Khamesipour, Y. Dowlati, S. Rafati and A.Z. Momeni et al., 2003. Immune response measured in human volunteers vaccinated with autoclaved Leishmania major vaccine mixed with low dose of BCG. Clin. Exp. Immunol., 134: 303-308.

6:  Srivastava, J.K., A. Misra, P. Sharma, B. Srivastava, S. Naik and A. Dube, 2003. Prophylactic potential of autoclaved Leishmania donovani with BCG against experimental visceral leishmaniasis. Parasitology, 127: 107-114.

7:  Modabber, F., 1989. Experiences with vaccines against coetaneous leishmaniasis of men and mice. Parasitology, 98: s49-s60.

8:  Mitchell, G., F.E. Handman, T.W. Spithill, 1984. Vaccination against coetaneous leishmaniasis in mice using nonpathogenic clonal promastigotes of leishmania major and importance of route of infection. Aust. J. Exp. Biol. Med. Sci., 62: 145-153.

9:  Daneshvar, H., G.H. Coombs, P. Hagan and R.S. Phillips, 2003. Leishmania mexicana and Leishmania major. attenuation of wild-type parasites and vaccination with the attenuated lines. J. Infect. Dis., 187: 1662-1668.

10:  Handman, E., 1990. Study of Leishmania major infected macrophages by use of lipophosphoglycan-specific monoclonal antibodies. Infect. Immun., 85: 2297-2302.

11:  Pinto, E.F., M. de Mello Cortezia and B. Rossi-Bergmann, 2003. Interferon-gamma-inducing oral vaccination with Leishmania amazonensis antigens protects BALB/c and C57BL/6 mice against coetaneous leishmaniasis. Vaccine, 21: 3534-3541.

12:  Kazemi, B., G.H. Tahvildar-Bideroni, S.R. Hashemi Feshareki and E. Javadian, 2004. Isolation a lizard Leishmania promastigote from its natural host in Iran. J. Biol. Sci., 4: 620-623.
CrossRef  |  Direct Link  |  

13:  Melby, P.C., 2003. Recent developments in leishmaniasis. Curr. Opin. Infec. Dis., 15: 485-490.

14:  Reed, S.G. and A. Campos-Neto, 2003. Vaccines for parasitic and bacterial diseases. Curr. Opin. Immunol., 15: 456-460.

15:  Hejazi, H., 1996. Immunologic studies of antigenic fragments of Leishmania major by biochemistry techniques. Ph.D. Thesis, Tarbiat Modarres University, Tehran, Iran.

16:  Kafetzis, D.A., 2003. An overview of paediatric leishmaniasis. J. Postgrad. Med., 49: 31-38.
CrossRef  |  Direct Link  |  

17:  Kalipada, K., 1995. Serodiagnosis of leishmaniasis. Crit. Rev. Microbiol., 21: 123-152.

18:  Murray, P.J., W.S.H. Terry and Handman, 1989. Characterization of integral membrane proteins of Leishmania major by Triton X-114. Fractionation and analysis of vaccination effects in Mice. Infec. Immun., 57: 2203-2209.

19:  Frommel, D., B.W. Ogunkolade, I. Vouldoukis and L. Monjour, 1988. Vaccine-induced immunity against coetaneous leishmaniasis in balb/C mice. Infect. Immunol., 56: 843-848.

20:  Cardoso, S.R., J.C. da Silva, R.T. da Costa, W. Mayrink and M.N. Melo et al., 2003. Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin). Rev. Soc. Bras. Med. Trop., 36: 193-199.

21:  Malekzadeh, S.H., M.H. Alimohammadian and H. Hosseyni, 1998. Partially induced protection by a fraction of Leishmania major promastigotes against murine leishmamiasis. Iranian Biomed. J., 2: 27-32.

22:  Kazemi, B., F. Moazzen, A. Abadi and A. Ghadjari, 2004. Fractionation of lizard Leishmania promastigote protein Fragment. Pak. J. Biol. Sci., 7: 1703-1705.
CrossRef  |  Direct Link  |  

©  2021 Science Alert. All Rights Reserved