Al- Shehri
Department of Biological Science, College of Science,
King Khalid University, Abha P.O. Box 9004, Saudi Arabia
M. Abdulrahman
Department of Biological Science, College of Science,
King Khalid University, Abha P.O. Box 9004, Saudi Arabia
Mostafa S. Yasser
Department of Biological Science, College of Science,
King Khalid University, Abha P.O. Box 9004, Saudi Arabia
ABSTRACT
Culture conditions affecting protease production by Bacillus licheniformis isolated from Tihamet Aseer, Saudi Arabia were investigated. The bacterium strain gave a maximum protease production of 221 U mL-1 when growing in a casein broth medium after 36 h compared with gelatin broth medium. Optimum pH and temperature of protease production were 8 and 50°C, respectively. The highest level of protease production in the presence of soybean meal as a carbon source and the peptone as a nitrogen source was obtained. The protease was optimally active at pH 9 and 55°C . The enzyme was stable at temperature range of 60-65°C during the period tested (1h) and retaining more than 85% of its activity at 70°C. These properties make the enzyme suitable for detergent industry.
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How to cite this article
Al- Shehri, M. Abdulrahman and Mostafa S. Yasser, 2004. Production and Some Properties of Protease Produced by Bacillus licheniformis
Isolated from Tihamet Aseer, Saudi Arabia. Pakistan Journal of Biological Sciences, 7: 1631-1635.
DOI: 10.3923/pjbs.2004.1631.1635
URL: https://scialert.net/abstract/?doi=pjbs.2004.1631.1635
DOI: 10.3923/pjbs.2004.1631.1635
URL: https://scialert.net/abstract/?doi=pjbs.2004.1631.1635
REFERENCES
- Feng, Y.Y., W.B. Yang, S.L. Ong, J.Y. Hu and W.J. Nig, 2001. Fermentation of starch for enhanced alkaline protease production by constructing an alkalophilic Bacillus pumilus strain. Applied Microbiol. Biotechnol., 57: 153-160.
Direct Link - Gupta, R., Q. Beg, S. Khan and B. Chauhan, 2002. An overview on fermentation, downstream processing of microbial alkaline proteases. Applied Microbiol. Biotechnol., 60: 381-395.
Direct Link - Calik, P., E. Bilir, G. Calik and T.H. Ozdamar, 2002. Influence of pH conditions on metabolic regulations in serine alkaline protease production by Bacillus licheniformis. Enzyme Microbial. Technol., 31: 685-697.
CrossRefDirect Link - Kim, J.M., W.J. Lim and H.J. Suh, 2001. Feather-degrading Bacillus species from poultry waste. Process. Biochem., 37: 287-291.
CrossRef - Joo, H.S., C.G. Kumar, G.C. Park, K.T. Kim, S.R. Paik and C.S. Chang, 2002. Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii. Process Biochem., 38: 155-159.
CrossRef - Nilegaonkar, S., P. Kanekar, S. Sarnaik and A. Kelkar, 2002. Production, isolation and characterization of extracellular protease of an alkaliphilic strain of Arthrobacter ramosus, MCM B-351 isolated form the alkaline lake of lonar, India. World J. Microb. Biotechnol., 18: 785-789.
Direct Link - Gaur, R., J. Yadav and L. Pandey, 1989. Thermostability of extracellular protease enzyme produced by Spicaria fusispora, a thermophilic fungus. Hindustan Antibiotics, 31: 36-37.
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