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Research Article
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Isolation of GSH from Baker Yeast by Autolysis Method |
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Mohamad Ramlan Bin Mohamed Salleh,
Yumi Zuhanis Has-Yun Binti Hashim ,
Rohani Binti Salleh
and
Haslinda Binti Hasim
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ABSTRACT
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The purpose of this study was to determine the effect of operating parameters
such as the yeast concentration, processing temperature and isolation time on
the isolation of GSH using Autolysis method. To study the effect of yeast concentration,
yeast solution with the concentration of 15, 30, 45 and 60 wt.% was disrupted
at their original temperature for 1 h. The result obtained shows that the optimum
value of the concentration for the Autolysis was 15 wt.% with the yield of 0.3942
μmol mL-1 GSH. To study the effect of processing temperature,
Autolysis process of the 15 wt.% yeast solution was done at a various values of
temperature such as 15, 25, 28, 31, 33 and 40°C. The results indicated that, the
optimum GSH isolation temperature (suitable processing temperature) is 28°C with
the yield of 1.367 μmol mL-1 GSH. Then, to study the effect of
isolation time, the Autolysis process of sample with the concentration of 15 wt.%
was done at 28°C for 1, 2 and 3 h. The results indicated that the optimum isolation
time for the Autolysis process is 1 h.
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CrossRef | Direct Link |
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