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Path Analysis and Relationship Among Quantitative Traits in Chickpea ( Cicer arietinum L.)



Falak Noor, Muhammad Ashraf and Abdul Ghafoor
 
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ABSTRACT

Phenotypic and genotypic variances, heritability in broad sense (h2), genetic advance (GA), correlation and path coefficient analyses were conducted for yield and yield components in 30 genotypes of chickpea under rainfed conditions. Medium to high genetic variance was observed for days to flowering, maturity, secondary branches and 100-seeds weight, whereas for other characters, low to medium heritablity (broad sense) was observed along with low to high genetic advance. Improvement of these traits thorough direct selection could be limited from germplasm used in the present study. Days to flowering, days to maturity and 100-seed weight exhibited high heritablity coupled with high genetic advance, hence crop improvement through these traits could be possible by simple selection. It was concluded that to improve grain yield emphasis should be given on development of chickpea cultivars with higher seed weight and biological yield. To break undesirable linkage of biological yield Vs harvest index and grain yield Vs harvest index, bi-parental mating among selective parents is suggested for further improvement. Both correlation and path analyses indicated that pods per plant and 100-seed weight were potent contributors to grain yield through direct effects. Seven elite accessions which produced higher grain yield than both the checks were selected and suggested for further evaluation under various ecological zones for adaptation.

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  How to cite this article:

Falak Noor, Muhammad Ashraf and Abdul Ghafoor , 2003. Path Analysis and Relationship Among Quantitative Traits in Chickpea ( Cicer arietinum L.). Pakistan Journal of Biological Sciences, 6: 551-555.

DOI: 10.3923/pjbs.2003.551.555

URL: https://scialert.net/abstract/?doi=pjbs.2003.551.555

Introduction

Chickpea is an ancient cultivated plant, domesticated during early age of civilization and being cultivated throughout the world. Its nomenclature in different countries is well documented as, gram, chickpea, hommos, chana, chieting vetch, nakhud, nakhut, kicher, pois chice, garbanzo, etc. (Malik, 1994). Chickpea probably originated in South-eastern Turkey and old Sanskrit names and carbonized seeds indicate the presence of chickpea on the India sub-continent as early as 2000 BC (Savithri, 1976). It is fifth most important among legumes in the world and being cultivated on more than 12 million hectares with annual production of 8 million tones. In Pakistan, it is cultivated on an area of 971,000 hactares with annual production of 564,000 tones with national average of 581 kg ha‾1. Our national average is very low as compared to its potential and yield obtained by other chickpea growing countries, and only one third of the potential is harvested (Ghafoor et al., 1997).

The extent of genetic variation is a pre-requisite in any crop improvement and yield is an end product of many field crops. Independent variables influence yield directly or indirectly and they are inter-related in a complex way (Singh et al., 1995). In a biological system most of the traits are associated with each other by simple or complex paths and to investigate the actual path for improving

yield potential is the task of breeders. Correlation analysis provides information on associated response of plant characters and therefore, leads to a directional model for yield prediction (Khan and Qureshi, 2001). Character association is very important study that has been conducted in chickpea using different material by Malik et al. (1988) Gull (1995) Bakhsh et al. (1998). Path coefficient analysis quantifies the inter-relationship among different variables keeping yield as dependant. The present studies were under-taken to find out inter-relationships among various quantitative traits and to investigate the most important traits contributing towards grain yield in chickpea.

Materials and Methods

Twenty eight accessions of chickpea along with two chickpea (Punjab 91 and Paidar 91) were evaluated for agronomic traits under field conditions at National Agricultural Research Centre, Islamabad, Pakistan (33.40°N and 73.07°E). The germplasm accessions were collected from three districts of Punjab (Khushab, Layyah and Bhakkar) and these were selfed to ensure homozygousity for two years prior to evaluation during 1999. The experiment was planted during winter season of 1999 in randomized complete block design (RCBD) in four replications. Two rows of 4 meter length for each genotype in each replicate were planted with 10 cm intra-row spacing, whereas inter-row distance was kept 50 cm. Pesticides and fungicides were sprayed to save the crop from infestation of pests and Asochyta rabiei. For evaluation, data were recorded following descriptors for chickpea (IBPGR, 1985). The data for days to flowering and maturity were recorded on line basis at 50% of flowering and 90% pod maturity and each genotype was represented by single value. Other quantitative data, i.e., plant height, primary and secondary branches, pods, grain yield (g) and biological yield (g) were recorded on ten plants sampled randomly. Seed weight was recorded after counting 100 seeds in grams and harvest index was determined as economic yield expressed in percentage over total biomass. The data recorded were analyzed for simple statistics, i.e., mean, standard deviation, variance and broad sense heritability. Broad sense heritability was estimated as a ratio between genotypic and phenotypic variance (Singh and Chowdhry, (1979). Correlation coefficients were calculated as followed by Al-Jibouri et al. (1958) whereas path coefficient analysis was conducted according to Dewey and Lu (1959).

Results and Discussion

Genetic variance, phenotypic variance, heritability and genetic advance presented in the Table 1 revealed high proportion of genetic variation for days to flowering, days to maturity, secondary branches and 100-seed weight. The range for days to flowering, days to maturity and number of primary branches was low, but due the adaptation of chickpea to Thall desert, the crop duration does not matter due to sole crop culture (Gull, 1995). For other characters, considerable range of the means was observed that indicated the scope of selection from these genotypes for crop improvement. Medium to high genetic variance was observed for days to flowering, maturity, secondary branches and 100-seed weight, whereas for other characters, low to medium heritability (broad sense) was observed. Improvement of these traits through simple selection might be limited from germplasm used in the present study. For the characters like, days to flowering, days to maturity and 100-seed weight, high heritability coupled with high genetic advance revealed the presence of additive gene effects, hence crop improvement through these improvement traits could be possible through simple selection (Ghafoor et al., 2000). Genetic advance along with heritability estimates gives an indication for gene-action and the characters with high heritability coupled with high genetic advance are supposed to be controlled additively, hence could be exploited through simple selection. Chickpea breeders should consider heritability estimates along with genetic advance because h2 alone is not a good indicator of the amount of usable genetic variability (Ghafoor et al., 1998).

Correlation coefficient analysis: The results regarding genotypic, phenotypic and environmental correlation coefficient given in the Table 2 revealed that the genotypic correlation’s were slightly higher than phenotypic ones for most of the characters, exhibiting high degrees of genetic association among traits under consideration. The environmental correlation coefficients were not much important in most of the cases except five combinations, i.e., primary branches Vs secondary branches, pods Vs biological yield, pods Vs grain yield biological yield Vs grain yield where it was positive and biological yield Vs harvest index where it was negative. The significant environmental correlation indicated environmental influence which is quite expected in a crop like chickpea (Malik and Tufail, 1984). The experiment was conducted under rainfed condition and hence environments played important role to determine correlation among characters, therefore, these results could only be valid for selection under rainfed conditions. Days to flowering exhibited significantly positive correlation with primary branches (0.5687), whereas negative with plant height (-0.5505), pods per plant (-0.7241) and harvest index (-0.8992). Short duration cultivars could be selected to improve the yield potential from present material. Days to maturity gave positive correlation with primary branches (0.7097) and harvest index (0.5097), whereas plant height had significant positive correlation with biological yield and negative with primary branches. 100-seed weight showed significant association with biological yield and grain yield. Grain yield was positively correlated with all the characters, except harvest index where it was significant, whereas it was insignificant negative with days to flowering and secondary branches. Genetic improvement in chickpea is mainly focused on grain yield by the breeders of the country (Bakhsh et al., 1998). Grain yield is a complex character which is the final product of many (some known and others unknown) independent variables. In the present study, grain yield was positively associated with biological yield and 100-seed weight but negatively with harvest index. To improve grain yield emphasis should be given on development of chickpea cultivars with higher seed weight and biological yield. The genotypes with low grain yield and high biological yield consequently produced low harvest index and this important combination, high biological yield and harvest index could be attained using bi-parental mating to break unwanted linkage for further improvement of the crop.

Table 1:
Basic statistics for 30 genotypes of chickpea evaluated during 1999
σ2g- genotypic variance, σ2p- phenotypic variance, σ2h- heritability (broad sense), GA- genetic advance at 10% selection differential

Table 2:
Genotypic correlation among ten quantitative traits in chickpea
DF-days to flowering, DM-days to maturity, PH-plant height (cm), PBR-primary brancher per plant, SBR-secodary brancher per plant, P/P-pods/plant, SW-100-seeds weight (g), BY-biological yield/plant (g), GY-grain yield plant (g)

Table 3:
Direct (parenthesis) and indirect effect of independent variables with dependent variable (grain yield). The last shows genotypic correlations of independent variables with grain yield

Table 4:
Performance of chickpea genotypes selected on the basis of evaluation during 1999
DF-Days to 50% flowering, DM-days to 90% maturity, PH-Plant height (cm), Pods-number of pods/plant, SW-100-seed weight, GY-Grain yield/plant (g), HI-Harvest indes (%). * Significant at 0.05 ** Highly significant at 0.001

Positive correlation of grain yield with branches, pods and eed weight has already been reported by Gull, 1995 and Bakhsh et al. (1998) that indicated the consistency of these associations in chickpea, hence could be exploited for crop improvement.

Path coefficient analysis: The genotypic correlation coefficients were partitioned into direct and indirect effects by various yield contributing characters (Table 3). The path coefficient analysis was carried out in this study to utilize a complete represent of the causal factors involved in determining the end product i.e., grain yield. The direct effects exhibited by secondary branches, pods and 100-seed weight were positive, whereas all the other characters gave negative direct effects. The highest direct effect of 1.9242 was exhibited by pods per plant and it was followed by secondary branches (1.2356) and 100-seed weight (1.2177). 100-seed weight and pods per plant also exhibited significant positive association with grain yield, hence could more confidently be exploited for crop improvement.

In the present study conducted under rainfed conditions indicated that pods per plant and 100-seed weight had the maximum contribution in determining grain yield, the ultimate product in chickpea under rainfed conditions. Further it was observed that high indirect contribution was exhibited via secondary branches and harvest index by most of the yield components, hence these two traits along with pods per plant and 100-seed weight are suggested to be given emphasis while selecting high yielding chickpea cultivars for rainfed conditions. Correlation and path coefficient analyses indicated that pods per plant and 100-seed weight were potent contributors to grain yield through direct effects. Although biological yield had significant association but exhibited negative direct effects, whereas Singh et al. (1995) reported high direct effects by biological yield, pods per plant and 100-seed weight. The contradiction for biological yield may be related to the experimental conditions as present study was conducted under rainfed conditions. On the basis of performance, seven accessions produced higher grain yield than both the checks, hence were selected for further evaluation under a wide range of environmental conditions (Table 4).

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