Subscribe Now Subscribe Today
Research Article
 

Effect of Seed Cleaning, Washing and Seed Treatment on Seedling Disease Incidence and Yield of Rice



M. Asad-ud-doullah, M.K. Anam, Md. Nazrul Islam, M. Rahman, G.A. Fakir and I. Hossain
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

Six treatments viz. farmer’s seed (uncleaned and unwashed), farmer’s clean seed, farmer’s washed seed, farmer’s clean and washed seed, garlic (1:10) and Vitavex-200 @ 0.3% treated seeds were used for studying their comparative effect on incidence of seedling diseases and grain yield of rice. Seed cleaning followed by washing decreased the seedling diseases viz. brown spot, blast, bakanae, foot rot and seedling blight as of seed treatment with garlic and Vitavex-200. Use of healthy seedling increased the grain yield up to 21.4% over the use of seedling as farmer’s normally use. Use of healthy seedlings has increased the benefit of Tk.4132.28 ha–1 over the use of normal seedling as farmers normally use.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

M. Asad-ud-doullah, M.K. Anam, Md. Nazrul Islam, M. Rahman, G.A. Fakir and I. Hossain, 2002. Effect of Seed Cleaning, Washing and Seed Treatment on Seedling Disease Incidence and Yield of Rice. Pakistan Journal of Biological Sciences, 5: 767-769.

DOI: 10.3923/pjbs.2002.767.769

URL: https://scialert.net/abstract/?doi=pjbs.2002.767.769

Introduction

In Bangladesh 14,076,000ha of land is under cultivation of different crops of which 72.50% is under rice cultivation (Fakir and Karim, 1999). Rice is known to be attacked by a large number of diseases of which forty five are seed borne (Richardson, 1990). Among the seed borne pathogen of rice, fungi are the most common. The pathogens may survive in seeds even with the healthy looking ones. Healthy looking seeds infected with pathogens are more dangerous. The badly infected seeds with expressed symptoms like spotting and discoloration can be recognized by visual observation. Detection of pathogens in healthy looking seeds is not easy at farmer’s level due to lack of testing facilities. Seed treatment with chemicals is costly and it is not friendly to environment. Moreover the farmer’s of Bangladesh do not get the chemicals easily. Further, they are not aware of the health of their seeds. According to Hasan (2000), physical sorting of seeds resulted in 8.33% increase of germination in rice seeds., Prevalence of seed borne fungal flora in physically sorted seeds was lower than farmer’s saved seeds resulting in higher percent germination (Islam et al., 2001). Therefore, the study was conducted in the farmer’s field with the following objectives:

1) To create the awareness of farmer’s on seed health and its impact on crop production.
2) To demonstrate the benefit of using healthy looking clean seed and washed seeds.
3) To make the farmer’s confidence of using easy technique(s) for controlling selected seed borne diseases of rice.

Materials and Methods

The present research work has been carried out in seed beds and fields of nine farmer’s at four different locations, namely Mirzapur, Boira, Gowripur and Shikarikanda of Mymensingh district in Bangladesh, during 2000. The topography of the experimental fields was medium high land belonging to the Sonatola series of grey flood soil type under old Brahmaputra flood plain (Anonymous, 1988). The chemical composition of soil was as follows:

An area of 40m2 was used in each farmer’s field for seedbeds and was divided into six units giving 0.5m space between the units, where each unit was used as a bed. Six different treatments viz. farmer’s seed (unclean and unwashed), farmer’s clean seed, farmer’s washed seed, farmer’s cleaned and washed seed, garlic extract at the ratio of 1:10 (garlic clove: water) and Vitavex-200 @ 0.3% treated seeds were used for raising the seedlings in the seed bed. For conducting field experiment, two groups of seedlings viz. seedlings, normally farmers use (with or without spot) and completely healthy looking seedlings were used. The seedlings of two groups have been transplanted in two different plots of each of the above mentioned farmer’s field. The area of each plot was 60m2. Data on grain yield was evaluated for each plot after harvesting the crop. The cost of seed cleaning and seedling selection was measured by taking into account the extra labour cost. Five woman labours @ Tk. 30.00 were required for seed cleaning, while six extra laborers @ Tk. 50.00 required for seedling selection for each hectare of rice field.

Results and Discussion

Seed cleaning, washing and seed treatment with garlic extract and Vitavex-200 showed significantly reduced seedling diseases of rice over the use of uncleaned and unwashed seeds as normally used by the farmers (Table 1). The 0.48 to 2.50% of seedlings showed brown spot symptom, while 0.27 to 1.71% of seedlings were recorded with blast disease symptom. Seedling showing bakanae, foot rot and seedling blight symptoms varied from 0.17 to 9.86%, 0.24 to 1.72% and 0.45 to 4.29%, respectively. Seed cleaning followed by washing showed statistically similar response in reducing the seedling diseases as of seed treatment with garlic extract (1:10) and Vitavex-200 (0.3%). Though the lowest seedling disease incidence was recorded with Vitavex-200 treated seeds, use of cleaned and washed seeds showed very good results as well (Table 1). Maximum reduction (86.07%) of seedling diseases was determined in case of using Vitavex-200, which was followed by garlic extract (74.82%). But seed cleaning and washing of farmer’s seed reduced the seedling disease up to 53.87% over the farmer’s uncleaned and unwashed seeds (Table 2).

The farmers of Bangladesh use about 94% required amount of seeds of their own stock or seeds of their neighbour by purchasing form the local hats or bazars (Fakir and Karim, 1999). The rest seed requirements (about 6% certified/quality seeds) are produced by Government, Semi-Government. organizations and NGO’s. Lack of good quality certified seeds to the farmers compels them to use their own seeds without having choice of seed selection. Under the present study emphasis has been given for using the farmers seeds just by adding simple, easy and adaptive methods of controlling seeds born pathogens in order to produce disease free healthy seedlings. Seed cleaning and washing resulted significant reduction of seedling diseases as of chemical treatment (Vitavex-200) over the uncleaned and unwashed farmer’s seeds.

Table 1:Effect of seed cleaning , washing and seed treatment on the incidence of seedling diseases of rice

Table 2:Comparative effect of seed cleaning, washing and treatment on the incidence of seedling diseases of rice over the uncleaned and unwashed seeds as normally farmer’s use
* Disease: Brown spot, Blast, Bakanae, Foot rot and Seedling blight

Table 3:Performance of completely healthy looking seedling and seedlings normally farmer’s use on the yield of rice
F= Farmer

Table 4:Benefit of seedling selection on income increase

This practice has increased the production of disease free healthy seedlings by 53.87% over the use of farmer’s uncleaned and unwashed seeds. This reduction of seedling diseases may be due to use of healthy looking, clean, disease free seeds and avoidance of using discoloured, light and chaffy grains. The discoloured, light and chaffy grains of rice are the good sources of seed-borne pathogens of brown spot, blast, foot root, bakanae and seedling blight (Agarwal et al., 1989; Fakir, 1999; Fakir, 1998). Siddiqui (1990) reported that certain diseases can be reduced by strict seed certification or avoidance of seed and soil infection. In the present study seed cleaning and washing helped in avoiding the use of light and chaffy grains. More over, seed cleaning and washing removed the infected plant debris, soil or other foreign materials from the seeds, thus resulting in the reduction of pathogens with the seeds.

Under present study the treatment of seeds with Garlic extract and Vitavex-200 caused 74.82 and 86.07% reduction in production of diseased seedlings respectively. This might be due to the control of seed-borne pathogens. The findings are supported by Hossain et al. (1997), Mia (1998) and Hossain et al. (1998). Hossain and Ashrafuzzaman (1994), Fakir and Jahan (1998), Ashrafuzzaman and Hossain (1995) and Hossain et al. (1997) reported that the crude extract of garlic (Allium sativum) showed marked effect in inhibition of spore germination, mycelial growth and pathogenicity of Bipolaris sorokiniana. Use of Vitavex-200 for controlling seed born pathogens in the present study showed an excellent result which is evident from the seedling stand reduction in seedling disease prevalence. This finding is also supported by the results of Islam et al. (1992), Mia (1998), Hossain et al. (1998), Hossain and Asad-ud-Doullah (1998), Fakir and Jahan (1998).

It was found that transplanting healthy looking disease free seedlings resulted in 4471.11kg ha–1 grain yield, while the normal seedlings as used by the farmers yielded 3898.33kg ha–1 grains (Table 3). This indicates that use of healthy looking seedlings increased the yield (250-870 kg ha–1) over the use of seedlings as normally farmer’s use. The results of the present study clearly show that use of healthy looking seedlings gave an average increase in rice yield of 14.77% (572.78 kg ha–1) giving an income increase of 4132.28 Bangladeshi Tk. ha–1 (US $ 86.01 ha–1, Table 4). According to Mathur et al. (1998) manual seed cleaning by farmers resulted in better looking, healthier crops and produced more grain yield and average increase in rice yield by 510kg ha–1, giving an income increase of Tk. 3060 ha–1 to each farmer.

In conclusion the present study showed an excellent direction of disease management without the use of chemicals. The farmers can clean and wash their seeds easily and after washing they can sow their seeds in the seedbed for growing healthy seedlings. This practice is environment friendly and help in avoiding the use of costly chemicals. Moreover, the poor farmers of the country can use this practice with little training and they can increase their income. So farmers can transplant the healthy seedlings for better yield by using the clean seeds without treating chemicals.

REFERENCES
1:  Agarwal, P.C., C.N. Mortensen and S.B. Mathur, 1989. Seed born diseases and seed health testing of rice. Technical Bulletin No. 3 and Phytopathological Papers No. 30, Copenhagen 1989, pp: 106.

2:  Anonymous, 1988. Production Year Book. Food and Agriculture Organization of United Nations, Rome, Italy, pp: 64.

3:  Ashrafuzzaman, H. and I. Hossain, 1995. Studies on plant disease control with crude plant extracts. BAU Res. Prog., 9: 79-84.

4:  Fakir, G.A. and R. Jahan, 1998. Control of major seed-born pathogens of Jute. Proceedings of the 1st National Workshop on Seed Pathology, Progress and Prospect of Seed Pathological Research in Bangladesh, June 9-12, Bangladesh Agricultural University, pp: 18-18.

5:  Fakir, G.A., 1999. An Annoted List of Seed-borne Disease in Bangladesh. Seed Pathology Laboratory, Department of Plant Pathology, BAU, Mymensingh, Bangladesh, pp: 100.

6:  Fakir, G.A., 1998. Health Status of Farmer`s Rice Seed. In: Progress and Prospect of Seed Pathological Research in Bangladesh, Hossain, I., G.A. Fakir and M.A. An (Eds.). Bangladesh Agricultural University, Mymensingh, Bangladesh.

7:  Fakir, G.A. and M.R. Karim, 1999. Seed pathology in Bangladesh and its future needs. Proceedings of the Workshop on the Future Strategy of the Danish Government Institute of Seed Pathology for Developing Countries, March 23-24, Copenhagen, Denmark, pp: 4-4.

8:  Hasan, M.M., 2000. Effect of seed cleaning and washing on germination, disease incidence and yield of rice. M.Sc. Thesis, Department of Plant Pathology, BAU, Mymensingh, Bangladesh.

9:  Hossain, I. and M. Asad-ud-doullah, 1998. Report on pilot project research. Proceedings of the DGISP Workshop II Held on Dec. 10th, at BARC, Dhaka.

10:  Hossain, I. and H. Ashrafuzamman, 1994. Control of Rhizoctonia solani and Bipolaris sorokiniana with crude plant extracts. BAU Res. Prog., 8: 86-92.

11:  Hossain, I., G.A. Fakir and M.A. Ali, 1998. Control of Bipolaris sorokiniana in wheat. Proceedings of the 1st National Workshop on Seed Pathology, Progress and Prospect of Seed Pathological Research in Bangladesh, June 9-12.

12:  Hossain, I., H. Mahamud and H. Ashrafuzamman, 1997. Effect of plant extracts on fungi (Bipolaris sorokiniana and Rhizoctonia solani) and okra mosaic disease. Ecoprint: Int. J. Ecol., 4: 35-42.

13:  Islam, M.R., M.A.T. Mia and M.A. Haque, 1992. Chemical control of seed-borne diseases of rice. Bangladesh J. Plant Pathol., 8: 13-16.

14:  Islam, S.M.A., I. Hossain, G.A. Fakir and M. Asad-du-doullah, 2001. Effect of physical seed sorting and seed treatment with garlic extract and Vitavex-200 on incidence of anthracnose and target spot of jute (Corchorus capsularis L.). Pak. J. Phytopathol., 13: 160-166.

15:  Mia, M.A.T., 1998. Leaf scald of rice. Proceedings of the 1st National Workshop on Seed Pathology, Progress and Prospect of Seed Pathological Research in Bangladesh, June 9-12.

16:  Richardson, M.J., 1990. An Annotated List of Seed-born Diseases. 3rd Edn., CAB International Mycological Institute, Kew, Survey, England.

17:  Siddiqui, M.R., 1990. Management of Seed-borne Diseases. Daya Publishing House, New Delhi, India, pp: 27.

18:  Mathur, S.B., A.M. Ali and G.A. Fakir, 1998. Transfer of seed health technology increases farmer`s income in Bangladesh-first trial. Seed Pathol. News, 27: 9-9.

©  2021 Science Alert. All Rights Reserved