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Research Article
 

Enhancement of Podophyllotoxin Production by Biogenetic Precursors and Elicitors in Cell Suspension Cultures of Juniperus chinensis



Duangporn Premjet , Kazutaka Itoh and Sanro Tachibana
 
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ABSTRACT

Juniperus chinensis suspension cultures were conducted in Schenk and Hildebrandt medium. On addition of phenylalanine and coniferyl alcohol to the cultures, podophyllotoxin production was enhanced 2.6 and 6-folds compared with the control, respectively. Chito-oligosaccharides, chitopentaose and methyl jasmonate stimulated cells to produce podophyllotoxin at 5, 5.2 and 1.5 times the control level, respectively. The combination of two elicitors, chitopentaose and methyl jasmonate, markedly increased 15-folds relative to the control.

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  How to cite this article:

Duangporn Premjet , Kazutaka Itoh and Sanro Tachibana , 2002. Enhancement of Podophyllotoxin Production by Biogenetic Precursors and Elicitors in Cell Suspension Cultures of Juniperus chinensis. Pakistan Journal of Biological Sciences, 5: 1267-1271.

DOI: 10.3923/pjbs.2002.1267.1271

URL: https://scialert.net/abstract/?doi=pjbs.2002.1267.1271

REFERENCES
1:  Ayres, D.C. and J.D. Loike, 1990. Lignans: Chemical, Biological and Clinical Properties. Cambridge University Press, Cambridge.

2:  Bodkin, F., 1986. Encyclopedia Botanica. Angus and Robertson Publishers, Australia.

3:  Broomhead, A.J. and P.M. Dewick, 1990. Aryltetralin lignans from linum flavum and linum capitatum. Phytochemistry, 29: 3839-3844.
Direct Link  |  

4:  Canel, C., R.M. Moraes, F.E. Dayan and D. Ferreira, 2000. Molecules of interest: Podophyllotoxin. Phytochemistry, 54: 115-120.
Direct Link  |  

5:  Ellis, B. and G.H.N. Towers, 1970. Biogenesis of rosmarinic acid in Mentha. Biochem. J., 118: 291-297.
CrossRef  |  Direct Link  |  

6:  Farmer, E.E., R.R. Johnson and C.A. Ryan, 1992. Regulation of expression of proteinase inhibitor gene by methyl jasmonate and jasmonic acid. Plant Physiol., 98: 995-1002.
CrossRef  |  PubMed  |  Direct Link  |  

7:  Fett-Neto, A.G., S.J. Melanson, S.A. Nicholson, J.J. Pennington and F. Di Cosmo, 1994. Improved taxol yield by aromatic carboxylic acid and amino acid feeding to cell cultures of Taxus cuspidata. Biotechnol. Bioeng., 44: 967-971.
PubMed  |  Direct Link  |  

8:  Fowler, M.W., 1988. Application of Plant Cell and Tissue Culture. John Wiley and Sons, Chichester.

9:  Fukuda, T., Y. Madoka and S. Yasuda, 1997. Formation and regulation of secon-dary metabolites in cell cultures of woody plants. I. Accumulation of catechin related substances and browning phenomenon in ginkgo callus. Mokuzai Gakkaishi, 43: 819-823.
Direct Link  |  

10:  Funk, C., K. Gugler and P. Brodelius, 1987. Increased secondary product formation in plant cell suspension cultures after treatment with a yeast carbohydrate preparation (Elicitor). Phytochemistry, 26: 401-405.
Direct Link  |  

11:  Hora, B., 1981. The Oxford Encyclopedia of Trees of the World. Oxford University Press, London.

12:  Jeffrey, W.P. and M.W. John, 1990. Plant Cell and Tissue Culture. Human Press, New Jersey.

13:  Kikkawa, Y., T. Kawada, I. Furukawa and T. Sakuno, 1990. A convenient preparation method of chito-oligosaccharides by acid hydrolysis. J. Fac. Agric. Tottori Univ., 26: 9-17.
Direct Link  |  

14:  Linden, J.C. and M. Phisalaphong, 2000. Oligosaccharides potentiate methyl jasmonate-induced production of paclitaxel in Taxus canadensis. Plant Sci., 158: 41-51.
CrossRef  |  PubMed  |  Direct Link  |  

15:  Misawa, M., 1994. Plant tissue culture: An alternative for production of useful metabolite. http://www.fao.org/docrep/t0831e/t0831e08.html.

16:  Miyata, M., K. Itoh and S. Tachibana, 1998. Extractives of Juniperus chinensis L. I: Isolation of podophyllotoxin and yatein from the leaves of J. chinensis. J. Wood Sci., 44: 397-400.
CrossRef  |  Direct Link  |  

17:  Moreno, P.R.H., R. van der Heijden and R. Verpoorte, 1993. Effect of terpenoids precursor feeding and elicitation on formation of indole alkaloids in cell suspension cultures of Catharanthus roseus. Plant Cell Rep., 12: 702-705.
CrossRef  |  

18:  Moreno, P.R.H., R. van der Heijden and R. Verpoorte, 1995. Cell and tissue cultures of Catharanthus roseus: A literature survey. II: Updating from 1988 to 1993. Plant Cell Tissue Org. Cult., 42: 1-25.
CrossRef  |  Direct Link  |  

19:  Muranaka, T., M. Miyata, I. Kazutaka and S. Tachibana, 1998. Production of podo-phyllotoxin in Juniperus chinensis callus cultures treated with oligosaccharides and a biogenetic precursor. Phytochemistry, 49: 491-496.
Direct Link  |  

20:  Nojiri, H., M. Sugimori, H. Yamane, Y. Nishimura and A. Yamada et al., 1996. Involvement of jasmonic acid in elicitor-induced phytoalexin production in suspension-cultured rice cells. Plant Physiol., 110: 387-392.
Direct Link  |  

21:  Premjet, D., I. Kazutaka and S. Tachibana, 2001. Production of Podophyllotoxin in Juniperus Chinensis Cell Suspension Cultures-Treated with Biogenetic Precursors and Chito-Oligosaccharides. Japan Wood Research Society, Tokyo.

22:  Premjet, D., I. Kazutaka and T. Sanro, 2002. Stimulation of the production of podophyllotoxin by biogenetic precursors and an elicitor in Juniperus chinensis stem-derived callus cultures. Pak. J. Biol. Sci., 5: 313-316.
CrossRef  |  Direct Link  |  

23:  Rosa, M.C., J. Palazon, A. Navia-Osorio, A. Mallol, M. Bonfill, C. Morales and M.T. Pinol, 1999. Production of taxol and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture. Plant Sci., 146: 101-107.

24:  Uehara, K., 1969. Jumoku Daizusetsu. 1st Edn., Ariake Shobo Publisher, Tokyo, pp: 36-43.

25:  Williams, S.D., R. Birch and L.H. Einhorn, 1987. Treatment of disseminated germ cell tumors with cisplatin, bleomycin and either vinblastine or etoposide. New Engl. J. Med., 316: 1435-1440.
Direct Link  |  

26:  Yoshida, M., M. Toshio, I. Kazutaka and T. Sanro, 2002. Stimulation of the production of taxol by oligosaccharides in Taxus cuspidate variety nana callus cultures. Pak. J. Biol. Sci., 5: 461-465.
CrossRef  |  Direct Link  |  

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