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Research Article
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Enhancement of Podophyllotoxin Production by Biogenetic Precursors and Elicitors
in Cell Suspension Cultures of Juniperus chinensis |
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Duangporn Premjet ,
Kazutaka Itoh
and
Sanro Tachibana
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ABSTRACT
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Juniperus chinensis suspension cultures were conducted in Schenk and Hildebrandt medium. On addition of phenylalanine and coniferyl alcohol to the cultures, podophyllotoxin production was enhanced 2.6 and 6-folds compared with the control, respectively. Chito-oligosaccharides, chitopentaose and methyl jasmonate stimulated cells to produce podophyllotoxin at 5, 5.2 and 1.5 times the control level, respectively. The combination of two elicitors, chitopentaose and methyl jasmonate, markedly increased 15-folds relative to the control.
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