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Anatomical Studies in Relation to Taxonomy of Persian Linum Species



Fariba Sharifnia and Rokneddin Mohammad Albouyeh
 
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ABSTRACT

The present survey was performed on twelve Persian Linum species with the aim to illustrate species inter-relationships and to evaluate the taxonomic treatments proposed for the genus Linum in Iran. It includes comparative anatomy of the species based on leaves and stems transverse sections. The results are highly in agreement with the previous morphologic based taxonomic treatments of the genus and anatomical traits used are efficient for application at generic and sub-generic levels of the genus Linum in Iran.

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  How to cite this article:

Fariba Sharifnia and Rokneddin Mohammad Albouyeh, 2002. Anatomical Studies in Relation to Taxonomy of Persian Linum Species. Pakistan Journal of Biological Sciences, 5: 1240-1245.

DOI: 10.3923/pjbs.2002.1240.1245

URL: https://scialert.net/abstract/?doi=pjbs.2002.1240.1245

Introduction

The genus Linum L. belongs to the family Linaceae S. F. Gray with about 230 species that are distributed throughout temperate regions of the world (Heywood, 1993). Rechinger (1974) has reported sixteen species from Iran Plateau in Flora Iranica and has divided the genus into five sections. However, Sharifnia and Assadi (2001) in the course of study of the genus Linum in Iran have reduced the number of species for flora of Iran and have relatively removed L. tenuifolium from section Linum and placed it in section Linastrum (Planch) H. Walker in Engler and Prantl. (Sharifnia and Assadi, 2001). Moreover, Sharifnia (2002) has conducted phenetic studies based on morphological characters of different Persian species in order to confirm the previous studies. Several researchers have studied Persian Linum species taxonomically, but they have been restricted to considering morphological traits (Parsa, 1951; Rechinger, 1974; Mobayen, 1995).

Anatomical studies have great implications for clarification of taxonomic relationships in higher ranks of classification. However, in some plant families, several anatomical traits are of great value for application at generic and sub-generic levels (Jones and Luchsinger, 1987).

Esau (1977) and Fahn (1989) have performed previous anatomical studies of the genus Linum with the emphasis on characteristics of bast fibers.

In this paper, we have conducted anatomical studies to indicate species inter-relationships, evaluate the previous taxonomic treatments of the genus Linum in Iran and to provide evidence for efficiency of application of these data at generic and sub-generic level of the genus.

Materials and Methods

We initiated the anatomical studies in the Laboratory Center of Tehran Science and Research Branch, Islamic Azad University with dried herbarium material which were previously collected from different regions of Iran during the summer of 1999 (Table 1) and revived them by boiling in water followed by cooling and fixation in F.A.A. {HCOOH: CH3COOH: C2H5OH (10:5:85 v/v)} as described by Azizian (1996).

All the chemicals were purchased from Merck, Germany.

For the hand sections, we chose the same site, which were taken from stems (beneath the terminal inflorescence) and leaves lamina in the species studied.

The methylene blue-carmine procedure was followed for staining as described by Albouyeh et al. (2002) and were studied the specimens with a light microscope, which was equipped with a camera Zeiss light microscope, model: standard 25 was used.

Table 1: list of Linum species used in anatomical studies, including their localities and accession numbers
* Voucher specimens are deposited in central herbarium of Iran (TARI)
** Placed as such in table 1 for the sake of simplicity in comparative study with L. strictum and L. corymbolusum

Results and Discussion

Our results of comparative leaf anatomy among members of section Linastrum suggest that they share the same characteristics including leaves with one vein, which has prominence in both leaf surfaces. The same is also true for L. tenuifolium and is another indicative for placement of L. tenuifolim in section Linastrum in Iran as suggested by Sharifnia (2002), (Fig. 1A, B, C).

Fig. 1:
Leaf transverse section of L. strictum X40 (A) and L. tenuifolium X40 (B) and L. corymbulosum X80 © pr= prominence

Fig. 2:
Stem transverse section of L. corymbulosum X 80 (A) and L. tenuifolium X 80 (B). ep=epidermis, c=cortex, xy=xylem, f=fibers, p= pith

However, stem comparative anatomy in section linastrum reveals some differences in thickness and density of different tissues among species studied (Fig. 2A, B).

Members studied from section Syllinum Griseb. have similar leaf anatomy with having one veined, one sided prominent leaves, except for L. album in which the prominence is less evident (Fig. 3, A, B). However, multi-circular appearance of stems in transverse sections is a common feature among species studied from section Syllinum and cannot be seen in other Linum species studied and therefore supports their membership together under a separate section (Fig. 4,A, B, C).

Anatomically, we refer to section Linum as a heterogeneous group. Variation in leaf anatomy is noticeable from leaves without prominence in L. peyronii (Fig. 5, A), leaves prominent in both sides (e.g. L. usitatissimum), (Fig. 5, B) to three veined leaves in L. nervosum. (Fig. 5, C). Stem transverse sections also represent variation from circular form (in L. nervosum), (Fig. 6, A) to waved form (L. peyronii), (Fig. 6, B). Stem epidermal cells can be seen in one layer (L. usitatissimum) (Fig. 7, A) and two layers (L. austriacum), (Fig. 7, B).

Fig. 3: Leaf transverse section of L. nodiflorum X 80 (A) and L. album X 80 (B) pr= prominence

Fig. 4: Stem transverse section of L. album X80 (A), L. nodiflorum X80 (B) and L. mucronatum X 40 m.c=multi-circular

Fig. 5: Leaf transverse sections of L. peyronii X 80 (A), L. usitatissimum X 40 (B), L. nervosum X 40 © and L. nervosum X 16 (D), pr=prominence, v= vein

Fig. 6: Stem transverse sections of L. nervosum X 40 (A) and L. peyronii X 40 (B), c= circular, w= wavy

Fig. 7:
Stem transverse sections of L. usitatissimum X 80 (A) and L. austriacum X 80 (B), ep=epidermis

Fig. 8:
Leaf transverse section of L. catharticum X 40 (A) and stem transverse section of L. cathariticum X 80 (B), ep= epidermis, ext.
s =   external surface, int.  s=   internal surface,   F= fibers

Fig. 9:
Leaf transverse section of L. densiflorum X 80 (A) and stem transverse section of L. densiflorum X 80 (B) e.h= epidermal hairs

Section Cathrolinum (Reichenb.) Planch. is monotypic with single taxonomically isolated species L. catharticum in which leaves are one veined without any prominence. The distinguishing features in stem anatomy are epidermal cells with thickened walls in both external and internal surfaces and connected phloem fibers (in contrast to more or less scattered fibers in other Linum species studied), (Fig. 8, A, B).

Section Dasylinum Planch. is again monotypic with the single taxonomically isolated species L. densiflorum. Covering of stem and leaves from long epidermal hairs, a feature not found in other Linum species studied, is anatomically fair enough evidence for isolation of L. densiflorum (Fig. 9, A, B).

In conclusion, our results highly support the previous taxonomical treatments of the genus Linum in Iran and show the selectivity of anatomical traits for application at the sub-generic level of the genus Linum in Iran and therefore represent the efficiency of anatomical studies for investigation into the taxonomy of Persian Linum species.

REFERENCES
1:  Albouyeh, R.M., A. Majd, H. Mirzaie-Nodoushan and M.B. Rezaie, 2002. Investigation of several anatomical characters in some Mentha species. Iran. Rangelands For. Breed. Genet. Res., 8: 81-93.

2:  Azizian, D., 1996. Anatomical studies on Mentha mozaffarianii (Labiatae) and related species. Iran. J. Bot., 7: 63-71.
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3:  Esau, K., 1977. Anatomy of Seed Plants. 2nd Edn., John Wiley and Sons Inc., San Francisco, USA., ISBN-13: 9780471245209, pp: 550.

4:  Fahn, A., 1989. Plant Anatomy. 4th Edn., Pergamon Press, Oxford, pp: 90-92.

5:  Heywood, V.H., 1993. Flowering Plants of the World. 1st Edn., Oxford University Press, Oxford, UK., pp: 67-69.

6:  Jones, S.B. and A.E. Luchsinger, 1987. Plant Systematics. 2nd Edn., McGraw Hill, New York, pp: 84-85.

7:  Mobayen, S., 1995. Flora of Vascular Plants of Iran. Tehran University Press, Tehran.

8:  Parsa, A., 1951. Flore De Iran. Tehran University Press, Tehran, pp: 1390-1392.

9:  Rechinger, K.H., 1974. Flora Iranica. Akademishe Druck University, Verlagsanstalt, Graz, Austria, pp: 1-3.

10:  Sharifnia, F., 2002. Revision of the genus Unum in Iran. Iran. J. Bot.

11:  Sharifnia, F. and M. Assadi, 2001. Linacea Flora of Iran. Research Institute of Forests and Rengelands, Tehran.

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