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Research Article
 

Isolation and Characterization of Bacterial Species from Surgical and Non-surgical Wounds Located on Body Surface of Buffaloes, Cattles, Sheep and Goats



Khan T.S. and R.Rind
 
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ABSTRACT

Bacteriae were isolated and characterized on biomorphological variations into eleven different species, (Streptococcus pyogenes, S. uberis, Staphylococcus aureus, S. intermedius, Corynebacterium diphtheriae C. pyogenes, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Micrococcus luteus and Stomatococcus mucilaginosus). They varied from cocci to rod shape and were gram-positive or gram-negative. Organisms produced a variety of colonies on different media. Some were spherical, swarming and spreading colonies on agar media while in broth, granular turbidity with powdery deposits were also seen. A few species produced hemolysis ( and ) of red blood cells. Biochemical activities of different organisms revealed that among two similar species of Streptococcus,S. pyogenes had not hydrolyzed aesculin but S. uberis had hydrolyzed aesculin. The other difference between two was that the first produced β hemolysis while the second produced . Staphylococcus aureus was coagulase positive, while S. intermedius was not. However, other two species Corynebacterium diphtheriae and C. pyogenes were also biochemically tested. The first had not liquefied gelatin while the other had.

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  How to cite this article:

Khan T.S. and R.Rind , 2001. Isolation and Characterization of Bacterial Species from Surgical and Non-surgical Wounds Located on Body Surface of Buffaloes, Cattles, Sheep and Goats. Pakistan Journal of Biological Sciences, 4: 696-702.

DOI: 10.3923/pjbs.2001.696.702

URL: https://scialert.net/abstract/?doi=pjbs.2001.696.702

REFERENCES

1:  Barnham, M. and D.J. Neilson, 1987. Group L, beta haemolytic streptococcal infection in meat handlers: Another streptococcal zoonosis. Epidemiol. Infect., 99: 257-264.
PubMed  |  

2:  Bergeys, D.H., 1992. Manual of Determinative Bacteriology. 7th Edn., Williams and Wilkins Co., Baltimore, pp: 336-583

3:  Breed, R.S., E.G.D. Murray and N.R. Smith, 1957. Bergey's Manual of Determinative Bacteriology. 7th Edn., Williams and Wilkins Co., Baltimore

4:  Cruickshank, R., 1968. Medical Microbiology: A Guide to the Laboratory Diagnosis and Control of Infection. 11th Edn., English Language Book Society and E.S. Livingstone Ltd., UK., pp: 820-828

5:  Cruickshank, R., 1970. Medical Microbiology. 12th Edn., Churchil Livingstone, London

6:  Laboratories, D., 1960. Difco Manual of Dehydrated Culture Media and Reagents for Microbiological and Clinical Laboratory Procedures. 9th Edn., Difco Laboratories, Detroit, Michigan, USA

7:  Dinev, D., K. Koichev, K. Kolev and E. Gerganova, 1987. Aetiology and chemotherapy of purulent surgical infection in horses and cattle. Veterinarnomeditski Naulei, 23: 51-59.

8:  Salle, A.J., 1988. Fundamental Principles of Bacteriology. 7th Edn., Tata Mcgraw-Hill Publishing Co. Ltd., New Delhi

9:  Shaikh, S.N., 1999. Bacteriological studies on the uteri of slaughtered goats. M.Sc. Thesis, Sindh Agriculture University, Tando Jam, Pakistan.

10:  Smith, D.T. and N.F. Conant, 1962. Zinssers Microbio. 5th Edn., Appleton-Century-Croft Inc., New York, pp: 244-248

11:  Talan, D.A., D. Staatz, A. Staatz, E.J. Goldstein, K. Singer and G.D. Overturf, 1989. Staphylococcus intermedius in canine gingiva and canine-inflicted human wound infections: laboratory characterization of a newly recognized zoonotic pathogen. J. Clin. Microbiol., 27: 78-81.
Direct Link  |  

12:  Wilson, G.S. and A.A. Miles, 1964. Tapley and Wilsons Principles of Bacteriology and Immunity. 5th Edn., Edward Arnold Publishers, London, pp: 636-801

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