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Research Article
 

Optimizing Recovery of Listeria Species from Imported Frozen Beef



Endang Purwati, Zaiton Hassan , Gulam Rusul , Raha Abdul Rahim and Son Radu
 
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ABSTRACT

The objective of present work was to optimize the procedures of FDA and USDA for the isolation of Listeria species in imported frozen beef samples marketed in Malaysia. The modifications consisted of direct analysis or storage of samples at 4°C for 24 h prior to analysis, and enrichment at 30°C or 35°C for 24, 48 and 168 h. For both FDA and USDA modified methods, storage at 4°C for 24 h and pre-enrichment at 24 and 48 h were the most efficient. However, the modified FDA with storage at 4°C for 24 h and pre-enrichment for 24 h (30°C and 35°C) and 48 h (30°C and 35°C) yielded more Listeria species. The rates of isolation were markedly affected with prolonged pre-enrichment incubation up to 168 h. The overall conclusion was that the modified USDA isolation method is beneficial when a limited range of the clinically important Listeria species is sought, whilst the modified FDA is needed to estimate the prevalence of Listeria species in the samples examined.

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  How to cite this article:

Endang Purwati, Zaiton Hassan , Gulam Rusul , Raha Abdul Rahim and Son Radu , 2001. Optimizing Recovery of Listeria Species from Imported Frozen Beef. Pakistan Journal of Biological Sciences, 4: 1547-1549.

DOI: 10.3923/pjbs.2001.1547.1549

URL: https://scialert.net/abstract/?doi=pjbs.2001.1547.1549

REFERENCES
1:  Brett, M.S.Y., P. Short and J. McLauchlin, 1998. A small outbreak of listeriosis associated with smoked mussels. Int. J. Food Microbiol., 43: 223-229.

2:  CDC, 1999. Update: Multi state outbreak of listeriosis-United States, 1998-1999. Morbidity Mortality Weekly Rep., 47: 1117-1118.
Direct Link  |  

3:  Donelly, C.W., 1999. Conventional Methods to Detect and Isolate Listeria monocytogenes. In: Listeria, Listeriosis and Food Safety, Ryser, E.T. and E.H. Marth (Eds.). Marcel Dekker Inc., New York, pp: 225-260.

4:  Endang, P., R. Son, H. Zaiton and G. Rusul, 1998. Antimicrobial drug resistance and resistance factor transfer among Listeria species. Asian Fish. Sci., 11: 261-270.

5:  Fenlon, D.R., J. Wilson and W. Donachie, 1996. The incidence and level of Listeria monocytogenes contamination of food sources at primary production and initial processing. J. Applied Bacteriol., 81: 641-650.

6:  Franco, P., D. Elaine, F. Jeff and W. Don, 2001. Isolation of Listeria monocytogenes from all food and environmental samples. http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/volume2/mfhpb30-01-eng.php.

7:  Harrison, M.A., W.H. Yao, H.C. Chia and S. Tiffany, 1991. Fate of Listeria monocytogenes on packaged, refrigerated and frozen sea food. J. Food Prot., 54: 524-527.

8:  Iida, T., M. Kanzaki, A. Nakama, Y. Kokubo, T. Maruyama and C. Kaneuchi, 1998. Detection of Listeria monocytogenes in humans, animals and foods. J. Vet. Med. Sci., 60: 1341-1343.

9:  Inoue, S., A. Nakama, Y. Arai, Y. Kokubo and T. Maruyama et al., 2000. Prevalence and contamination levels of Listeria monocytogenes in retails foods in Japan. Int. J. Food Microbiol., 59: 73-77.

10:  Lawrence, L.M. and A. Gilmour, 1994. Incidence of Listeria sp. and Listeria monocytogenes in a poultry-processing environment and in poultry and their rapid confirmation by multiplex PCR. Applied Environ. Microbiol., 60: 4600-4604.

11:  Lovett, J. and A.D. Hitchins, 1988. Listeria isolation: Revised method of analysis. Fed. Registrat., 53: 44148-44153.

12:  Lovett, J. and A.D. Hitchins, 1991. Listeria isolation: Supplement to FDA Bacteriological Manual. 6th Edn., AOAC, New York.

13:  McClain, D. and W.H. Lee, 1988. Development of USDA-FSIS method for isolation of L. monocytogenes from raw meat and poultry. J. AOAC., 71: 660-663.

14:  McClain, D. and W.H. Lee, 1989. Method for Isolation and Identification of L. monocytogenes from Meat and Poultry Products. Vol. 57, United States Department of Agriculture, Washington, DC.

15:  McLauchlin, J., S.M. Hall, S.K. Velami and R.J. Gilbert, 1991. Human listeriosis and pate: A possible association. Br. Med. J., 303: 773-775.

16:  Peterson, L. and M. Madsen, 2000. Listeria spp. in broiler flocks: Recovery rates and species distribution investigated by conventional culture and the EiaFoss method. Int. J. Food Microbiol., 58: 113-116.
Direct Link  |  

17:  Ryu, C.H., S. Igimi, S. Inoue and S. Kumagai, 1992. The incidence of Listeria spp. in retail foods in Japan. Int. J. Food Microbiol., 16: 157-160.

18:  Uyttendaele, M., P. de Troy and J. Debevere, 1999. Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the Belgian retail market. J. Food Prot., 62: 735-740.

19:  Wilson, I.G., 1995. Occurrence of Listeria species in ready-to-eat foods. Epidemiol. Infec., 115: 519-526.

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