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Research Article
 

Identification and In vitro Antimicrobial Activity of a Compound Isolated from Streptomyces Species



Zakia Sultana Sathi , Md. Aziz Abdur Rahman and M.A. Gafur
 
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ABSTRACT

A pure antimicrobial compound was isolated from the chloroform extract of the broth culture of Streptomyces species by preparative thin layer chromatography (PTLC) and was characterized as 4-hydroxy nitrobenzene (ZS-3) on the basis of spectral data (UV, IR, 1H-NMR, HMBC and HMQC). The compound showed significant antimicrobial activity against six gram positive bacteria, ten gram negative bacteria and three pathogenic fungi. The minimum inhibitory concentration (MIC) of the compound determined against ten pathogenic organisms was found to be between 32-64 g/ml.

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  How to cite this article:

Zakia Sultana Sathi , Md. Aziz Abdur Rahman and M.A. Gafur , 2001. Identification and In vitro Antimicrobial Activity of a Compound Isolated from Streptomyces Species. Pakistan Journal of Biological Sciences, 4: 1523-1525.

DOI: 10.3923/pjbs.2001.1523.1525

URL: https://scialert.net/abstract/?doi=pjbs.2001.1523.1525

REFERENCES
1:  Stahl, E., 1969. Thin Layer Chromatography: A Laboratory Handbook. 2nd Edn., Springer, New York, USA.

2:  Hammond, S.M. and P.A. Lambert, 1978. Antimicrobial Actions. Edward Arnold Ltd., London, pp: 4-9.

3:  John, G.H., N.R. Kreig, H.A.P. Smeath, J.T. Staley and S.T. Williams, 1994. Bergays Manual of Determinative Bacteriology. 9th Edn., Springer, New York, pp: 605-702.

4:  Routin, J.B. and A.C. Finlay, 1952. Problems in the search for microorganisms producing antibiotics. Bacteriol. Rev., 16: 51-67.
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5:  Reiner, R., 1982. Detection of Antibiotic Activity Antibiotics: An Introduction. Vol. 2, Roche Scientific Service, Switzerland, pp: 21-25.

6:  Sathi, Z.S., 2000. Chemical and Biological studies on Metabolites of Streptomyces and Nocardiopsis species. M. Pharm. Thesis, University of Rajshahi, Bangladesh.

7:  Pretsch, E., 1983. Spectral Data for Structure Determination of Organic Compound. Springer Verlag Berlin, Heidelberg, ISBN: 3540124063.

8:  Bauer, A.W., W.M.M. Kirby, J.C. Sherris and M. Turck, 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45: 493-496.
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