Five liquid media were studied for the production of dry weight spores of Fusarium oxysporum f. sp. ciceris and pH/ phytotoxicity of the culture filtrates after each 7 days incubation up to 28 days. Maximum increase in pH was observed in minimal medium (MM) at 21 days. The media potato dextrose broth (PDB) and minimal medium containing chickpea root extract (MM+) were found suitable for sporulation of the fungus, while maximum dry weight of the fungus was obtained in PDB medium. Phytotoxicity assay of these culture filtrates revealed that the maximum activity was produced in PDB medium, while ethyl acetate phases of the culture filtrates from Czapek dox (CZ) medium and minimal medium (MM) showed maximum phytotoxic activity.
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Iftikhar A. Khan, S.S. Alam and A. Jabbar, 2001. Standardization of Medium for the Production of Maximum Phytotoxic Activity by Fusarium oxysporum f. sp. ciceris. Pakistan Journal of Biological Sciences, 4: 1374-1376.
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