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Pakistan Journal of Biological Sciences

Year: 2001 | Volume: 4 | Issue: 11 | Page No.: 1299-1302
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Research Article

Diagnosis of Copper Deficiency Through Growth, Nutrient Uptake and Some Biochemical Reactions in Pisum sativum L.

Zeinab A. Salama

ABSTRACT

A water culture experiment was carried out on Pisum sativum L. ( cv. Master lee) cultivar to test and compare the possible use of changes in growth (as dry weight), nutrient uptake, nutrient concentration shoot /root ratios, (super oxide dismutase SOD (EC. 1. 15. 1.1) and ascorbate oxidase AO (EC.1. 10. 3.3) activities, chlorophyll content, proton release mechanism and protein profiles as parameters for diagnosis of Cu deficiency. Copper was supplied in two levels (Zero- 1μmol) as CuCl2. The results indicate that sufficient level of Cu caused increases in shoot and root dry weights as well as nutrient uptake. Mg, P, Fe, Mn and Cu concentration shoot/root ratios showed pronounced increases. In contrary the Zn shoot/root ratio was decreased. Under Cu deficient conditions, the activities of SOD and AO were reduced by 28 and 68% as well as proton release mechanism (71%). Moreover total chlorophyll was reduced by (49%) as a result of absence of Cu in the growth root medium. On the other hand protein profiles showed over expression of two high molecular weight bands at 94kD and 67kD with high intensity and one band at 43 kD were recorded. Therefore, study appreciated the possible use of proton release, AO level, chlorophyll content, Cu-specific changes in SDS-PAGE proteins and SOD level as rapid and sensitive biochemical assays to assess the Cu nutrition status in pea plants.
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