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Research Article
 

A New Alkaline alpha-Amylase from the Facultative Alkalophile Verticillium lateritium



Amany H.A.Abo Ellil
 
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ABSTRACT

An alkaline alpha-amylase was recorded, for the first time, as an extracellular product of the facultative alkalophile, Vertcillium lateritium, isolated from alkaline Egyptian soil. The enzyme was highly produced at pH 10.5 on dextrin which stimulated its formation more than starch. A positive relation was found between sodium ion concentration and enzyme production and fungal growth. Carbonate ions induced enzyme formation and the enzyme production was accelerated by Sodium carbonate. Alkaline alpha-amylase was produced during the exponential phase of fungal growth and the maximum yield was obtained after 4 days incubation. In presence of techopherol and ascorbic acid, as antioxidants, the maximum yield of the enzyme was evident in the 4 hours and 5th day of growth. This denotes that the exponential phase could be elongated to release the highest amount of the alkaline enzyme.

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  How to cite this article:

Amany H.A.Abo Ellil , 1999. A New Alkaline alpha-Amylase from the Facultative Alkalophile Verticillium lateritium . Pakistan Journal of Biological Sciences, 2: 301-304.

DOI: 10.3923/pjbs.1999.301.304

URL: https://scialert.net/abstract/?doi=pjbs.1999.301.304

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